A5100766019- Glycosylation and Glycoproteins Research
- Ferroptosis and cancer prognosis
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Carbohydrate Chemistry and Synthesis
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Galectins and Cancer Biology
- Cancer-related Molecular Pathways
- Hedgehog Signaling Pathway Studies
- Molecular Biology Techniques and Applications
- Cellular transport and secretion
- Electron and X-Ray Spectroscopy Techniques
- Wnt/β-catenin signaling in development and cancer
- Biotin and Related Studies
- Glycogen Storage Diseases and Myoclonus
- Berberine and alkaloids research
- Congenital heart defects research
- Genomic variations and chromosomal abnormalities
- Transplantation: Methods and Outcomes
- Lysosomal Storage Disorders Research
- Calcium signaling and nucleotide metabolism
- Chromatin Remodeling and Cancer
First Affiliated Hospital of Gannan Medical University
2025
Children's Hospital of Philadelphia
2019-2024
Second Affiliated Hospital of Jilin University
2013-2023
Chongqing Medical University
2022
Max Planck Institute for Evolutionary Anthropology
2022
Leipzig University
2022
Fudan University
2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2020
University of Pennsylvania
2019
Collaborative Innovation Center of Chemistry for Energy Materials
2017
Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into formaldehyde solution, which cross-link protein DNA molecules tissue cell structure. Here we provide a step guide performing this procedure mouse.
Recent studies demonstrated that pyroptosis plays a crucial role in shaping the tumor-immune microenvironment. However, influence of on lower-grade glioma regarding immunotherapy and targeted therapy is still unknown. This study analyzed variations 33 pyroptosis-related genes normal tissues. Our found considerable genetic expression alterations heterogeneity among gliomas brain There are two phenotypes glioma, they exhibited differences cell infiltration characteristics clinical characters....
Congenital disorders of glycosylation (CDG) represent 1 the largest groups metabolic with >130 subtypes identified to date. The majority CDG are N-linked glycosylation, in which carbohydrate residues, namely, N-glycans, posttranslationally linked asparagine molecules peptides. To improve diagnostic capability for CDG, we developed and validated a plasma N-glycan assay using flow injection-electrospray ionization-quadrupole time-of-flight mass spectrometry.After PNGase F digestion...
Breast cancer is the most common malignant in women. CYP24A1 expression regulates cellular response to vitamin D, which has antitumor effects against breast cancer. This study aimed identify correlation between mRNA and prognosis of enrolled 1102 patients, including 1090 females 12 males, from TCGA-BRCA cohort. The Cancer Genome Atlas database was used cancer, Chi-squared tests were performed test clinical features expression. prognostic value assessed using Kaplan-Meier curves Cox analysis....
Liver cancer is a highly malignant tumor, and patients typically have poor prognoses. Metabolic reprogramming hallmark of cancer, downregulation oxoglutarate dehydrogenase-like (OGDHL) contributes to the onset progression several cancers. We examined role altered OGDHL expression in liver determined its value as diagnostic prognostic indicator for patients.R (version 3.5.1) R extensions were used data mining The Cancer Genome Atlas (TCGA) dataset (including RNAseq clinical information)...
The enzyme carboxyl ester lipase (CEL), known as bile salt-dependent (BSDL) or salt-stimulated (BSSL), is mainly expressed in pancreatic acinar cells and lactating mammary glands. To investigate the link between CEL expression of breast cancer (BC) tissues survival BC patients by analyzing Cancer Genome Atlas Breast Carcinoma (TCGA-BRCA) level 3 data. clinical information RNA-sequencing (RNA-Seq) data were downloaded from TCGA. Patients divided into a high group low using optimal cutoff...
Ovarian cancer is a common gynaecological with poor prognosis that poses serious threat to human life and health. It essential explore the possible prognostic biomarkers of ovarian cancer. As an important tumour suppressor gene, BCL7A actively participates in growth tumours. We aimed study role cancer.Through data mining RNAseq from Cancer Genome Atlas database (TCGA), we explored clinical relevance mRNA expression. result, found expressed at low levels tissues correlated survival status....
SMC1A (structural maintenance of chromosomes 1A), which encodes a structural subunit the cohesin protein complex, is necessary for process sister chromatid cohesion during cell cycle. Mutation and deregulation are highly relevant to diverse human diseases, including Cornelia de Lange syndrome malignant carcinomas. In order further investigate role in oncogenesis lung cancer, SMC1A-specific short hairpin RNA (shRNA)-expressing lentivirus (Lv-shSMC1A) was constructed used infect A549 H1299...
LCN1 (lipocalin-1), a gene that encodes tear lipocalin (or von Ebner's gland protein), is mainly expressed in secretory glands and tissues, such as the lachrymal lingual gland, nasal, mammary, tracheobronchial mucosae. Analysis of Cancer Genome Atlas (TCGA) Breast Carcinoma (BRCA) level 3 data revealed relationship between expression survival breast cancer patients.The χ2 test Fisher exact were applied to analyze clinical RNA sequencing data, association clinicopathologic features was...
FUT8-CDG is a severe multisystem disorder caused by mutations in FUT8, encoding the α-1,6-fucosyltransferase. We report on dizygotic twins with presenting dysmorphisms, failure to thrive, and respiratory abnormalities. Due phenotype, oral L-fucose supplementation was started. Glycosylation analysis using mass spectrometry indicated limited response fucose therapy while clinical presentation stabilized. Further research needed assess concept of substrate FUT8-CDG.
Several studies implicate that lung cancer progression is governed by the interaction between epidermal growth factor receptor (EGFR) signaling and protein kinase C (PKC) pathways. Combined targeting of EGFR PKC may have an additive or synergistic effects in treatment. The aim this study to explore potential utility inhibiting these two pathways with combination erlotinib chelerythrine chloride non-small cell (NSCLC) lines. erlotinib-less sensitive lines SK-MES-1 A549 were treated themselves...
A new model using a gold nanoparticle (AuNP)-DNA system to constrain leakage and improve efficiency of catalytic toehold-mediated strand displacement reactions was outlined. 10-bp spacer on AuNPs fourfold amount fuels were determined for good performance this with an optimized toehold strategy. After the reaction at 25 °C 10 h, 258 pM target could be identified, which is remarkable improvement compared traditional AuNP-DNA without fuel. Moreover, also studied differentiate specific single...
Background: liver cancer is a malignant tumor with high morbidity and mortality that endangers human health. High mobility group A2 (HMGA2) chromosome associated protein participates in embryogenesis, tissue development, tumorigenesis development. Objective: to explore the relationship between HMGA2 expression clinicopathological parameters survival of patients using The Cancer Genome Atlas Liver Hepatocellular Carcinoma (HCC) data. Methods: RNA-sequencing data corresponding clinical...
Background We attempted to examine the clinical characteristics in patients with breast cancer (BC) and thyroid (TC); explore potential mechanisms of tumorigenesis progression. Methods Using Surveillance, Epidemiology, End Result Program-9 (SEER-9) database, a retrospective study (1975-2017) was conducted on BC TC. identified common differentially expressed genes involved TC using Gene Expression Omnibus database (GEO). Immunohistochemical staining (IHC) performed verify expression hit gene...
Abstract Ovarian cancer has the highest mortality among gynecological cancers. Although ovarian usually responds well to chemotherapy, most patients still have a poor prognosis. EIF2B5 is crucial molecule in posttranscriptional modifications involved tumor progression, and here we investigated prognostic role of cancer. We examined differential expression mRNA by exploring The Cancer Genome Atlas (TCGA) database. chi square test was used identify clinical correlation. Survival analysis Cox...
Breast cancer (BC) is the most common female malignant tumor worldwide. The mechanism of tumorigenesis still unclear. Ras‑related proteins in brain (Rab)22a belongs to Ras superfamily, which may act as an oncogene and participate carcinogenesis. present study aims identify whether Rab22a could be a novel biomarker prognosis determine effects on BC cell progression. A total 258 56 para‑tumor or non‑tumor formalin fixed paraffin embedded tissues were stained through immunohistochemistry....
Wntless (Wls) is an essential protein that necessary for the secretion of Wnt proteins. While numerous researches have demonstrated aberrations in Wnt/β-catenin expression lead to tumorigenesis and progression many cancer types, effects Wls breast (BC) are less studied.The mRNA BC cell lines were detected by RT-qPCR Western blot; patient samples was immunohistochemistry (IHC). The associations between clinicopathological factors as well survival time, including overall (OS) disease-free...
Background. ZNF385B, a zinc finger protein, has been known as potential biomarker in some neurological and hematological studies recently. Although numerous have demonstrated the function of proteins tumor progression, effects ZNF385B breast cancer (BC) are less studied. Methods. The Oncomine database “ESurv” tool were used to explore differential expression pan-cancer. Furthermore, data patients with BC downloaded from Cancer Genome Atlas (TCGA). receiver operating characteristic (ROC)...