A5065762813- Renal and related cancers
- Renal cell carcinoma treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Childhood Cancer Survivors' Quality of Life
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Pluripotent Stem Cells Research
- Advanced X-ray and CT Imaging
- Renal Diseases and Glomerulopathies
- Blood disorders and treatments
- Autoimmune and Inflammatory Disorders Research
- Digital Radiography and Breast Imaging
- Biomedical Ethics and Regulation
- Advanced Glycation End Products research
- Dietary Effects on Health
- Immunodeficiency and Autoimmune Disorders
- Pediatric Urology and Nephrology Studies
- Cancer Cells and Metastasis
- Genetic and Kidney Cyst Diseases
- Risk and Safety Analysis
- Congenital Diaphragmatic Hernia Studies
- Systemic Lupus Erythematosus Research
- Ethics and Legal Issues in Pediatric Healthcare
- Neonatal Respiratory Health Research
- Acute Lymphoblastic Leukemia research
Tel Aviv University
2012-2025
Tel Aviv Sourasky Medical Center
2022-2025
Bar-Ilan University
2023-2024
Sheba Medical Center
2012-2023
Edmond and Lily Safra Children's Hospital
2012-2023
Soreq Nuclear Research Center
2023
Israel Atomic Energy Commission
2023
Institute of Nanotechnology
2023
Schneider Children's Medical Center
2022
Boston Children's Hospital
2015
Wilms Tumor, the most common pediatric kidney cancer, evolves from failure of terminal differentiation embryonic kidney. Here we show that overexpression heterochronic regulator Lin28 during development in mice markedly expands nephrogenic progenitors by blocking their final wave differentiation, ultimately resulting a pathology highly reminiscent tumor. Using lineage-specific promoters to target specific cell types, observed tumor only when is aberrantly expressed multiple derivatives...
An open question remains in cancer stem cell (CSC) biology whether CSCs are by definition at the top of differentiation hierarchy tumor. Wilms' tumor (WT), composed blastema and differentiated renal elements resembling nephrogenic zone developing kidney, is a valuable model for studying this because early kidney well characterized. WT neural adhesion molecule 1-positive (NCAM1+) aldehyde dehydrogenase (ALDH1+) have been recently isolated shown to harbor progenitor traits. Herein, generating...
Abstract When assembling a nephron during development multipotent stem cell pool becomes restricted as differentiation ensues. A faulty arrest in this process leads to transformation and initiation of Wilms’ tumor. Mapping these transitions with respective surface markers affords accessibility specific subpopulations. NCAM1 CD133 have been previously suggested mark human renal progenitor populations. Herein, using sorting, RNA sequencing, vitro studies serum-free media vivo...
Systemic lupus erythematosus (SLE) comprise a diverse range of clinical manifestations. To date, more than 30 single gene causes lupus/lupus like syndromes in humans have been identified. In the setting, identifying underlying molecular diagnosis is challenging due to phenotypic and genetic heterogeneity.We employed whole exome sequencing (WES) patients presenting with childhood-onset severe and/or atypical presentations identify cases that are explained by single-gene (monogenic) cause.From...
Congenital anomalies of the kidney and urinary tract (CAKUT) are most common cause CKD in first three decades life. However, for patients with CAKUT, causative mutation remains unknown. We identified a kindred an autosomal dominant form CAKUT. By whole-exome sequencing, we heterozygous truncating (c.279delG, p.Trp93fs*) nuclear receptor interacting protein 1 gene ( NRIP1 ) all seven affected members. encodes transcriptional cofactor that directly interacts retinoic acid receptors (RARs) to...
Purpose Osteosarcoma (OS), the most common primary bone malignancy in childhood poses a therapeutic challenge despite extensive research. Neutrophil extracellular traps (NETs) play role tumor microenvironment (TME) variety of cancers, but their OS has not been characterized. Experimental Design This retrospective cohort study aimed to investigate immune cell infiltration and NETs formation patients with its association chemotherapy response overall survival using immunofluorescence...
Abstract X-ray imaging is a prevalent technique for non-invasively visualizing the interior of human body and other opaque samples. In most commercial modalities, an image formed by measuring X-rays that pass through object interest. However, despite potential scattered radiation to provide additional information about object, it often disregarded due its inherent tendency cause blurring. Consequently, conventional modalities do not measure or utilize these valuable data. contrast, we...
Cancer stem cell (CSC) identification relies on transplantation assays of subpopulations sorted from fresh tumor samples. Here, we attempt to bypass limitations abundant source and predetermined immune selection by in vivo propagating patient-derived xenografts (PDX) human malignant rhabdoid (MRT), a rare lethal pediatric neoplasm, an advanced state which most cells behave as CSCs. Stemness is then probed comparative transcriptomics serial PDXs generating gene signature epithelial...
The development of the mammalian kidney is a highly complex process dependent upon interplay various cell types, secreted morphogens, and extra-cellular matrix (ECM). Although integrins are most important receptors for ECM proteins ubiquitously expressed during development, mice lacking expression integrin α3 (Itga3) do not demonstrate reduced number nephrons, but mostly disorganized GBM (glomerular basement membrane) leading to proteinuria. Thus, ITGA3 considered passive stabilizer an...
Abstract Cancer stem cells (CSC) form a specific population within the tumor that has been shown to have self-renewal and differentiation properties, increased ability migrate metastases, resistance chemotherapy. Consequently, even small number of remaining after therapy can repopulate cause recurrence disease. CSCs in Wilms tumor, pediatric renal cancer, were previously be characterized by neural cell adhesion molecule (NCAM) expression. Therefore, NCAM provides biomarker through which CSC...
Nephrons are the functional units of kidney. During kidney development, cells from cap mesenchyme-a transient kidney-specific progenitor state-undergo a mesenchymal to epithelial transition (MET) and subsequently differentiate into various cell types that create tubular structures nephron. Faults in this can lead pediatric malignancy called Wilms' tumor mimics normal development. While human development has been characterized at gene expression level, comprehensive characterization...
Abstract Ewing sarcoma (EWS) is a highly aggressive cancer with survival rate of 70%–80% for patients localized disease and under 30% those metastatic disease. Tumor‐infiltrating neutrophils (TIN) can generate extracellular net‐like DNA structures known as neutrophil traps (NETs). However, little about the presence prognostic significance tumor‐infiltrating NETs in EWS. Herein, we investigated 46 diagnosed EWS treated Tel Aviv Medical Center between 2010 2021. TINs were identified diagnostic...
Abstract Pleuropulmonary blastoma (PPB) is a rare pediatric lung neoplasm that recapitulates developmental pathways of early embryonic lungs. As development proceeds with highly regulated mesenchymal-epithelial interactions, DICER1 mutation in PPB generates faulty differentiation program resultant biphasic tumors composed primitive epithelial and mesenchymal stroma progenitor blastomatous cells. Deciphering progression has been hampered by the difficulty culturing cells, specifically Here,...
Neutrophils are central players in the innate immune system. To protect against invading pathogens, neutrophils can externalize chromatin to create neutrophil extracellular traps (NETs). While NETs critical host defense, they also have deleterious effects, and dysregulation of formation has been implicated autoimmune diseases, atherosclerosis thrombotic conditions, cancer progression dissemination, acute respiratory distress syndrome. Here, we report that selinexor, a first-in-class...
Abstract Background Juvenile idiopathic arthritis (JIA) is the most common chronic rheumatic disease in children, causing significant morbidity. Despite dramatic improvement treatment, many patients do not achieve complete remission, and biomarkers for subclinical disease, flares, response to treatment are lacking. Neutrophils neutrophil extracellular traps (NETs) play key roles pathogenesis of autoimmune inflammatory conditions. In this study, we characterized enzyme activity NETs formation...
Resident physicians on long-term night shifts often face sleep deprivation, affecting the immune response, notably neutrophils, vital to innate defense mechanisms. Sleep-deprived residents exhibit altered neutrophil counts and reduced phagocytosis NADPH oxidase activity, critical combating infections. Our study focused extracellular traps (NETs), a process against pathogens not previously linked loss. Results revealed that sleep-deprived had significantly lower NETs formation compared...
Abstract X-ray imaging is a prevalent technique for non-invasively visualizing the interior of human body and opaque instruments. In most commercial modalities, an image formed by measuring X-rays that pass through object interest. However, despite potential scattered radiation to provide additional information about object, it often disregarded due its inherent tendency cause blurring. Consequently, conventional modalities do not measure or utilize these valuable data. contrast, we propose...
ABSTRACT Nephrons are the functional units of kidney. During kidney development, cells from cap mesenchyme – a transient kidney-specific progenitor state undergo mesenchymal to epithelial transition (MET) and subsequently differentiate into various cell types that create tubular structures nephron. Faults in this can lead pediatric malignancy called Wilms’ tumor mimics normal development. While development has been characterized at gene expression level, comprehensive characterization...
Abstract BACKGROUND AND AIMS Genetic etiologies are estimated to affect ∼10% of adults with advanced CKD. However, significant population disparities in genetic kidney disease exist and population-based screening lacking especially among minority groups. The Druze is a middle-eastern high rates end-stage renal (ESRD) consanguinity, which suggests population-specific monogenic CKD etiologies. We therefore hypothesized that by exome sequencing we will identify unique distribution ESRD causes...