Polyploid decidual cells are specifically differentiated during mouse uterine decidualization. However, little is known about the regulatory mechanism and physiological significance of polyploidization in pregnancy. Here we report a novel role E2F8 mice. highly expressed regulated by progesterone through HB-EGF/EGFR/ERK/STAT3 signaling pathway. transcriptionally suppresses CDK1, thus triggering cells. E2F8-mediated response to stresses which accompanied Interestingly, not detected human...

Embryo implantation into the maternal uterus is a decisive step for successful mammalian pregnancy. Osteopontin (OPN) member of small integrin-binding ligand N-linked glycoprotein family and participates in cell adhesion invasion. In this study, we showed that Opn mRNA levels are up-regulated mouse on day 4 at sites days 5 8 Immunohistochemistry localized OPN protein to glandular epithelium decidual zone proteins induced by vivo vitro decidualization. expression endometrial stromal cells...

OPN is essential for blastocyst implantation and placentation. Previous study found that miR181a was increased while miR181b downregulated in endometrium during decidualization. However, the information regarding their effects on decidualization human still limited. Here, we report a novel role of uterine pregnancy success humans. The expression high secretory phase vitro decidualized hESC, whereas low identical conditions. Further analysis confirmed upregulated by cAMP C/EBPβ signal...

Uterine vasodilation dramatically increases during the follicular phase of estrous cycle and pregnancy, which are estrogen-dominant physiological states. is believed to be mainly controlled by local uterine artery (UA) production vasodilators angiogenic factors. The extremely potent vasodilator proangiogenic hydrogen sulfide (H2S) synthesized via metabolizing L-cysteine cystathionine β-synthase (CBS) γ-lyase (CTH). This study was designed determine if UA H2S with augmented expression and/or...

The pregnancy-augmented uterine vasodilation is linked to increased AT 2 R (angiotensin type-2 receptor) that mediates the vasodilatory effects of angiotensin II. However, mechanisms controlling expression during pregnancy remain unclear. Estrogens are known play a role in vascular adaptations pregnancy. We hypothesized estrogen stimulates artery via ER (estrogen receptor)-β-dependent transcription pregnancy-specific endothelium-dependent manner. Plasma estradiol levels and peaked late...

Abstract Angiogenesis is a physiological process for endometrial regeneration in the menstrual cycle and remodeling during pregnancy. Endogenous hydrogen sulfide (H2S), produced by cystathionine-β synthase (CBS) cystathionine-γ lyase (CSE), potent proangiogenic factor; yet, whether H2S system expressed endometrium plays role angiogenesis are unknown. This study was to test estrogens stimulate biosynthesis promote microvascular endothelial cell (EMEC) angiogenesis. CBS messenger RNA/protein...

The prevalence of diabetes is increasing worldwide with the trend patients being young and creating a significant burden on health systems, including reproductive problems, but effects embryo implantation are still poorly understood. Our study was to examine mouse implantation, providing experimental basis for treating its complications. Streptozotocin (STZ) applied induce type 1 from day 2 pregnancy or pseudopregnancy in mice. Embryo transfer used analyze uterine environment implantation....

Endogenous hydrogen sulfide (H2 S), synthesized by cystathionine β-synthase (CBS) and γ-lyase (CSE), is a potent vasodilator that can be stimulated estradiol-17β (E 2 β) in uterine artery (UA) smooth muscle (UASMC) vivo; however, the underlying mechanisms are unknown. This study tested hypothesis E β stimulates H S biosynthesis upregulating CBS expression via specific estrogen receptor (ER). Treatment with time- concentration- dependent CSE messenger RNA (mRNA) protein expressions,...

Endogenous hydrogen sulfide (H2S) is a potent vasodilator and proangiogenic second messenger synthesized from L-cysteine by cystathionine β-synthase (CBS) γ-lyase (CTH). Estrogens are vasodilators that stimulate H2S biosynthesis in uterine arteries (UA) vivo; however, the underlying mechanisms unknown. We hypothesized estrogens UA endothelial cells (UAEC) via specific estrogen receptor (ER)-dependent mechanisms. In cultured primary UAEC, treatment with estradiol-17β (E2β) stimulated CBS CTH...

Objective This study aimed to compare the pregnancy outcomes between women receiving frozen embryo transfer (FET) with hormone replacement treatment (HRT) and without gonadotropin-releasing agonist (GnRHa) pretreatment. Methods All consecutive undergoing HRT cycles (2936 cycles) or GnRHa pretreatment (HRT + GnRHa, 303 at our reproductive center January 2015 December 2017 were analyzed retrospectively. Results The average age was higher in compared group (34.0 ± 4.8 vs. 31.3 4.4). However,...

Embryo implantation is a highly synchronized bioprocess between an activated blastocyst and receptive uterus. In mice, successful relies on the dynamic interplay of estrogen progesterone; however, key mediators downstream these hormones that act competency endometrium receptivity acquisition are largely unknown. this study, we showed expression osteopontin (OPN) in mouse blastocysts regulated by ovarian uterine micro-environment. OPN mRNA up-regulated day 4 pregnancy, which associated with...

A cutaneous squamous cell carcinoma (cSCC) derived from keratinocytes is the second most common cause of non-melanoma skin cancer. The accumulation mutational burden genes and cellular DNA damage caused by risk factors (e.g., exposure to ultraviolet radiation) contribute aberrant proliferation formation a cSCC. cSCC encompasses spectrum diseases that range recursor actinic keratosis (AK) (SCC) in situ (SCCIS) invasive cSCCs further metastatic SCCs. Emerging evidence has revealed lncRNAs are...

This study aims to analyze the cycle characteristics, pregnancy, and neonatal outcomes in early rescue intracytoplasmic sperm injection (r-ICSI) cycles normal hyper-ovarian response women their first IVF/ICSI attempts. Data from short-term vitro fertilization (IVF, N = 7148), r-ICSI (N 618), ICSI 1744) were retrospectively analyzed who underwent at our center October 2015 2021. The group was subdivided into partial 451) total 167) based on number of fertilized oocytes IVF part. Cyclic...

BACKGROUND:This study aims to explore the effect of Sinomenine (SIN) on pregnancy outcomes recurrent spontaneous abortion (RSA) in a mouse model. MATERIAL AND METHODS:Thirty female CBA/J mice were allocated into 3 groups randomly, then mated with BALB/c (CBA/J×BALB/c) as normal-pregnancy group (n=10), or DBA/2 (CBA/J×DBA/2) RSA model CBA/J×DBA/2 treated SIN RSA+SIN (n=10). The number surviving and reabsorbed embryos each counted day 13.5 gestation. serum was collected determine levels...