A5016256717- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Muscle Physiology and Disorders
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Adipose Tissue and Metabolism
- Sperm and Testicular Function
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Ovarian function and disorders
- Muscle metabolism and nutrition
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Xenotransplantation and immune response
- Genetics, Aging, and Longevity in Model Organisms
- Ginseng Biological Effects and Applications
- Reproductive System and Pregnancy
- Pancreatic function and diabetes
- Cellular Mechanics and Interactions
- Diabetes and associated disorders
- Healthcare and Venom Research
- Sirtuins and Resveratrol in Medicine
- Blood properties and coagulation
- RNA and protein synthesis mechanisms
Yanbian University
2014-2023
Huazhong University of Science and Technology
2010
Kindai University
2002
The host genome may influence the composition of intestinal microbiota, and microbiota has a significant effect on muscle growth development. In this study, we found that deletion myostatin (MSTN) gene positively regulates expression tight junction-related genes TJP1 OCLN through myosin light-chain kinase/myosin light chain pathway. structure MSTN-/- pigs differed from wild-type, including by presence thicker muscularis longer plicae. Together, these changes affect microbiota. Mice...
Enucleated oocytes matured in vitro, from which chromosomes were removed by treatment with ionomycin and demecolcine, used as recipient for nuclear transfer of fibroblast cells a mature male rabbit. The enucleated donor nuclei electrically activated 2 h after fusion. potential nuclear-transferred vitro ovulated to develop into blastocysts was high (33-55%), except cultured 8.0 (19%) 8.5 (25%) vitro. After recipients, ten 62 (16%) one eight (13%) recipients that received vitro-matured...
Glycine, a component of glutathione (GSH), plays an important role in protection from reactive oxygen species (ROS) and inhibition apoptosis. The aim this study was to determine the effect glycine on vitro maturation (IVM) porcine oocytes their developmental competence after parthenogenetic activation (PA). We examined nuclear maturation, ROS levels, apoptosis, mitochondrial membrane potential (ΔΨm), ATP concentration, as well expression several genes related oocyte development. Our studies...
Abstract BACKGROUND Myostatin ( MSTN ) negatively regulates skeletal muscle development; however, its functions in internal organs have not been thoroughly investigated. Here, we compared the morphological, molecular, and biological characteristics of heart, liver, spleen, lungs, kidneys, tongue homozygous mutant (MSTN −/− ), heterozygous +/− wild‐type (WT) piglets. RESULTS The heart liver were lighter piglets than piglets, while was heavier WT P < 0.05). Furthermore, longer myofibers...
Abstract Few studies exist on homozygous myostatin gene mutant ( MSTN −/− ) pigs, especially their reproductive ability. We have previously shown that semen quality of boars is comparable to wild type (WT). However, no data the ability heterozygous +/ − sows. The present study highlights showed sows delayed pubertal age than WT (255.80 ± 6.79 versus 191.10 3.42, respectively). number services per pregnancy significantly higher (3.33 0.43 1.60 0.25, Moreover, although natural reproduction...
Myostatin (MSTN) is a growth and differentiation factor that regulates proliferation of myoblasts, which in turn controls skeletal muscle growth. It may regulate myoblast by influencing miRNA expression, the present study aimed to clarify its precise mechanism action. Here, we found MSTN–/– pigs showed an overgrowth upregulated miR-455-3p level. Intervention MSTN expression using siMSTN C2C12 myoblasts also significantly increased miR-455-3p. was directly targeted 3′-untranslated region...
Baicalin, a monomer of flavonoids extracted from dried roots Scutellaria baicalensis, is used to treat female infertility. However, the effect baicalin on oocyte maturation unknown. In this study we investigated effects IVM pig oocytes and subsequent embryo development following parthenogenetic activation (PA). We found that 0.1µgmL-1 significantly (P<0.05) increased rate compared with non-treatment (control) group by reducing levels reactive oxygen species (ROS). addition, mRNA expression...
Epigenetic reprogramming and somatic cell nuclear transfer (SCNT) cloning efficiency were recently enhanced using histone deacetylase inhibitors (HDACis). In this study, we investigated the time effect of CI994, an HDACi, on blastocyst formation rate, acetylation levels H3K9 H4K12, DNA methylation anti-5-methylcytosine (5mC), some mRNA expression pluripotency-related genes in pig SCNT embryos. Treatment with 10 μM CI994 for 24 hours significantly improved rate embryos comparison untreated...
Baicalin, a traditional Chinese medicinal monomer whose chemical structure is known, can be used to treat female infertility. However, the effect of baicalin on embryonic development unknown. This study investigated effects in vitro parthenogenetically activated (PA) and fertilized (IVF) pig embryos underlying mechanisms involved. Treatment with 0.1 μg/ml significantly improved (P < 0.05) developmental capacity PA by reducing reactive oxygen species (ROS) levels apoptosis increasing...
Demecolcine (DEM) treatment of oocytes induces formation a membrane protrusion containing mass condensed maternal chromosomes, which can be removed with minimal damage prior to somatic cell nuclear transfer (SCNT). However, the effect this method on distribution maturation-promoting factor (MPF) in porcine has not been reported. Here, level MPF and cyclin B1 were assessed following DEM treatment. In addition, efficiencies DEM-assisted mechanical enucleation compared, as development (in vitro...
// Jin-Dan Kang 1, * , Hyojin Kim 2, 4, Long Jin Qing Guo 1 Cheng-Du Cui Wen-Xue Li Seokjoong 3 Jin-Soo 2 and Xi-Jun Yin Jilin Provincial Key Laboratory of Transgenic Animal Embryo Engineering Department Science, Yanbian University, Yanji 133002, China Center for Genome Engineering, Institute Basic Gwanak-gu, Seoul 151-747, South Korea ToolGen, Inc., Byucksan Kyoungin Digital Valley 2-Cha, Geumcheon-Gu, 153-023, 4 Present/Current address: Biosystems Science ETH Zurich, Basel CH-4058,...
Myostatin (MSTN) is a member of the transforming growth factor-β superfamily that negatively regulates skeletal muscle development. A lack MSTN induces hypertrophy and increases formation fast-twitch (Type II) fibres. This study investigated development in newborn heterozygous (MSTN+/−) homozygous (MSTN−/−) MSTN-knockout piglets. Detailed morphological gene protein expression analyses were performed biceps femoris, semitendinosus diaphragm MSTN+/−, MSTN−/− wild-type (WT) Haematoxylin–eosin...
Abstract Panax ginseng , a functional food, has been widely used as an edible nourishment and medicinal supplement. Ginsenoside Rb1 is major bioactive ingredient of ginseng, which shows very specific anti‐apoptosis anti‐oxidant activities. Methylglyoxal (MGO) one intermediate products glucose metabolism, absorbed easily from high sugar foods or carbonated beverages. It may involve in variety detrimental processes vivo. However, it not fully explored the effects ginsenoside on MGO‐induced...