A5079878817- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Protein purification and stability
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- Glioma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Cancer Research and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Spectroscopy and Chemometric Analyses
- Animal Genetics and Reproduction
- Immune Response and Inflammation
- Spectroscopy and Laser Applications
- Cancer Cells and Metastasis
- Anesthesia and Neurotoxicity Research
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related gene regulation
- Neonatal and fetal brain pathology
- Viral gastroenteritis research and epidemiology
- Cancer Mechanisms and Therapy
- Glycosylation and Glycoproteins Research
- Tissue Engineering and Regenerative Medicine
- Plasmonic and Surface Plasmon Research
Xinxiang Medical University
2016-2025
Columbia University
2025
First Affiliated Hospital of Xinxiang Medical University
2024
Jilin University
2021-2024
Jilin Agricultural University
2021-2024
Beijing University of Posts and Telecommunications
2024
University of Science and Technology Beijing
2024
Harbin Engineering University
2021-2023
Shaanxi Normal University
2019-2021
Dalian Maritime University
2020
The aim of this study was to investigate the effect downregulating Hedgehog pathway by GANT61 on human glioma cells, examine consequent changes temozolomide (TMZ)-induced effects and explore molecular mechanisms.The cytotoxicity a Gli1/2 inhibitor, examined both alone in combination with TMZ cell lines. mRNA protein expression alterations were determined quantitative real-time polymerase chain reaction (qRT-PCR) Western blot, respectively. CCK-8 assay detected proliferative capability....
Abstract In our previous study, we demonstrated that episomal vectors based on the characteristic sequence of matrix attachment regions (MARs) and containing cytomegalovirus (CMV) promoter allow transgenes to be maintained episomally in Chinese hamster ovary (CHO) cells. However, transgene expression was unstable number copies low. this focused enhancers, various promoters variants could improve stability, magnitude (level) copy a MAR‐based vector CHO‐K1 comparison with CMV promoter,...
Plasmon polaritons are formed by coupling light with delocalized electrons. The half-light and half-matter nature of plasmon endows them unparalleled tunability via a range parameters, such as dielectric environments carrier density. Therefore, expected to be tuned when in proximity polar materials since the density is an electrostatic potential; conversely, polariton response might enable sensing polarization. Here, we use infrared nanoimaging nanophotocurrent measurements investigate...
The aim of this study was to investigate the effects progesterone (PROG) on blood-brain barrier (BBB) permeability, cerebral edema and expression matrix metalloproteinase-9 (MMP-9) aquaporin-4 (AQP-4) in neonatal rats with hypoxic-ischemic brain damage (HIBD) explore mechanism its neuroprotective effect. Sixty 7-day-old Wistar were divided into sham surgery, hypoxic ischemia (HI) drug prophylaxis groups. HIBD animal models established. All animals sacrificed after 24 h. BBB assessed using...
Matrix attachment regions (MARs) are cis-acting DNA elements that can increase transgene expression levels in a CHO cell system. To investigate the effects of MAR combinations on and underlying regulatory mechanisms, we generated constructs which enhanced green fluorescent protein (eGFP) gene flanked by different human β-interferon β-globin (iMAR gMAR, respectively), was driven cytomegalovirus (CMV) or simian virus (SV) 40 promoter. These were transfected into CHO-K1 cells, screened with...
Abstract Liver cancer is a major malignant tumor, which seriously threatens human health and increases the economic burden on patients. At present, gene therapy has been comprehensively studied as an excellent therapeutic measure in liver treatment. Oncolytic virus (OV) kind of that can specifically infect kill tumor cells. After being modified by genetic engineering, specificity OV infection to cells increased, its influence normal reduced. To date, shown effectiveness safety experimental...
DNA molecular weight standard control, also called marker (ladder), has been widely used in the experiments of biology. In paper, we report a method by which was prepared based on multiple PCR technique. 100–1000 bp fragments were amplified using primers designed according to 6631 ~ 7630 position lambda DNA. Target Touchdown combined with hot start PCR, respectively, followed extracted phenol/chloroform, precipitated ethanol and mixed thoroughly. The results showed that successfully obtained...
A fixation-free and fast protein-staining method for sodium dodecyl sulfate–polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that conventional Brilliant Blue G phosphoric acid the solution. In addition, dye stain does not contain any amount methanol, such as acid. Considering speed, simplicity, low cost, may more practical value than other...
This aim of this study was to investigate whether progesterone (PROG) alleviates the neuronal apoptosis in neonatal rats with hypoxic-ischemic (HI) brain damage through phosphatidylinositol 3-kinase (PI3K)/Akt/glycogen synthase kinase-3β (GSK-3β) signaling pathway. A total 96 newborn Wistar aged 7 days were randomly divided into four groups: sham surgery, HI, drug prevention and Akt inhibitor groups. HI animal models established by a conventional method. All animals sacrificed 24 h after...
Chinese hamster ovary (CHO) cells are common mammalian cell lines for expressing recombinant proteins, yet the expression level of proteins is still hindered. Vector optimization and line modification key factors to improve proteins. In this study, vector was optimized by adding regulatory elements Kozak Leader upstream target gene detect transient stable Results indicated that with addition significantly increased compared control group. addition, inhibition apoptotic pathway has great...
Hepatitis B virus (HBV) X protein (HBx) is implicated in the development of hepatocellular carcinoma (HCC). Yes-associated 1 (YAP) an important proto-oncogene, which a downstream effector molecule Hippo signaling pathway. The aim present study was to investigate association between HBx expression HCC samples and YAP A total 20 pathologically confirmed samples, corresponding adjacent non-tumor liver tissues 5 normal tissue were collected. analyzed by quantitative reverse...
For the advantages of low cost, excellent thermal insulation, and sound absorption properties, rigid isocyanate-based polyimide foam (RPIF) presents great application prospects as a building insulation material. However, its inflammability accompanying toxic fumes create huge safety hazard. In this paper, reactive phosphate-containing polyol (PPCP) is synthesized employed with expandable graphite (EG) to obtain RPIF use safety. EG can be considered an ideal partner for PPCP weaken drawbacks...