A5046695122- RNA modifications and cancer
- Ginseng Biological Effects and Applications
- Cancer-related molecular mechanisms research
- Natural product bioactivities and synthesis
- Ferroptosis and cancer prognosis
- Pharmacological Effects of Natural Compounds
- Traditional Chinese Medicine Analysis
- Metabolomics and Mass Spectrometry Studies
- Phytochemistry and Biological Activities
- Alkaloids: synthesis and pharmacology
- Cancer-related gene regulation
- Berberine and alkaloids research
- Microbial bioremediation and biosurfactants
- Mangiferin and Mango Extracts
- Liver Disease Diagnosis and Treatment
- Ginger and Zingiberaceae research
- Catalytic C–H Functionalization Methods
- Pulmonary Hypertension Research and Treatments
- Advanced biosensing and bioanalysis techniques
- Plant-based Medicinal Research
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Drug-Induced Hepatotoxicity and Protection
- Hippo pathway signaling and YAP/TAZ
- Oxidative Organic Chemistry Reactions
Shanghai Institute of Materia Medica
2016-2025
Chinese Academy of Sciences
2016-2025
Third Xiangya Hospital
2025
Central South University
2017-2025
Shanghai Chest Hospital
2020-2024
Shanghai Jiao Tong University
2020-2024
National Center for Drug Screening
2024
Changzhou University
2021-2024
University of Chinese Academy of Sciences
2016-2021
Shanghai Normal University
2021
The biological functions of N6-methyladenosine (m6A) RNA methylation are mainly dependent on the reader; however, its role in lung tumorigenesis remains unclear. Here, we have demonstrated that m6A reader YT521-B homology domain containing 2 (YTHDC2) is frequently suppressed adenocarcinoma (LUAD). Downregulation YTHDC2 was associated with poor clinical outcome LUAD. decreased a spontaneous LUAD mouse model. Moreover, exhibited antitumor activity human cells. Mechanistically, YTHDC2, via...
Resistance to ferroptosis, a regulated cell death caused by iron-dependent excessive accumulation of lipid peroxides, has recently been linked lung adenocarcinoma (LUAD). Intracellular antioxidant systems are required for protection against ferroptosis. The purpose the present study was investigate whether and how extracellular system desensitizes LUAD cells ferroptosis.Established human fibroblasts MRC-5, WI38, H1650, PC9, H1975, H358, A549, H1299 lines, tumor matched normal adjacent...
Rationale: Ferroptosis, a newly identified form of regulated cell death, can be induced following the inhibition cystine-glutamate antiporter system XC- because impaired uptake cystine. However, outcome accumulation endogenous glutamate in lung adenocarcinoma (LUAD) has not yet been determined. Yes-associated protein (YAP) is sustained by hexosamine biosynthesis pathway (HBP)-dependent O-linked beta-N-acetylglucosaminylation (O-GlcNAcylation), and glutamine-fructose-6-phosphate transaminase...
Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer. Patient prognosis poor, and existing therapeutic strategies for LUAD are far from satisfactory. Recently, targeting N6-methyladenosine (m6A) modification RNA has been suggested as a potential strategy to impede tumor progression. However, roles m6A in tumorigenesis unknown.Global levels expressions writers, erasers readers were evaluated by methylation assay, dot blot, immunoblotting, immunohistochemistry ELISA human LUAD,...
Aims: Lipid peroxidation occurring in lung adenocarcinoma (LUAD) cells leads to ferroptosis. Lysophosphatidylcholine acyl-transferase 3 (LPCAT3) plays a key role providing raw materials for lipid by promoting esterification of polyunsaturated fatty acids phospholipids. Whether LPCAT3 determines ferroptosis sensitivity and the mechanism which its expression is regulated LUAD has not been reported. Results: acyl-coenzyme A (CoA) synthetase long-chain family member (ACSL)4 levels were...
Pulmonary arterial hypertension (PAH) is an incurable disease initiated by endothelial dysfunction, secondary to vascular inflammation and occlusive pulmonary remodeling, resulting in elevated pressure right heart failure. Previous research has reported that dysfunction of type 2 bone morphogenetic protein receptor (BMPR2) signaling pathway endothelium inclined prompt PAH models, but the underlying mechanism BMPR2 deficiency-mediated needs further investigation. This study was designed...
Ferroptosis is a new form of regulated cell death and closely related to cancer. However, the mechanism underlying regulation ferroptosis in lung adenocarcinoma (LUAD) remains unclear. IB, IHC ELISA were performed analyze protein expression. RT‑qPCR was used mRNA Cell viability, 3D growth, MDA, generation lipid ROS Fe2+ concentration measured evaluate responses induction ferroptosis. Measurement luciferase activity ChIP promoter by transcriptional regulator. Co‑IP assays identify...
Abstract Circulating microRNAs (miRNAs) can be used as noninvasive biomarkers and are also found circulating in body fluids such blood. Dysregulated miRNA expression is associated with many diseases, including non‐small cell lung cancer (NSCLC), the assay helpful diagnosis, prognosis, monitoring. In this work, a versatile electrochemical biosensing system developed for detection by DNAzyme‐cleavage cycling amplification hybridization chain reaction (HCR) amplification. With cleavage Mn 2+...
A lack of promising targets leads to poor prognosis in patients with lung adenocarcinoma (LUAD). Therefore, it is urgent identify novel therapeutic targets. The importance the N6-methyladenosine (m6A) RNA modification has been demonstrated various types tumors; however, knowledge m6A-related proteins LUAD still limited. Here, we found that insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3), an m6A reader protein, highly expressed and associated prognosis. IGF2BP3 desensitizes...
Ferroptosis is an iron- and lipid peroxidation-dependent form of regulated cell death. The release labile iron one the important factors affecting sensitivity to ferroptosis. Yes-associated protein (YAP) controls intracellular levels by transcription ferritin heavy chain (FTH) transferrin receptor (TFRC). However, whether YAP regulates metabolism through other target genes remains unknown. Here, we observed that system Xc- inhibitor erastin inhibited binding WW domain PSY motif between...
YAP is a transcriptional co-activator with critical roles in tumorigenesis. However, its upstream regulatory mechanism, especially how mRNA stability regulated, remains to be further studied. Here, we validated that expression was higher lung adenocarcinoma (LUAD) tissues compared adjacent normal tissues, and found m5C modification occurred 328-331 3' UTR region under the promotion NSUN2 ALYREF, increased of mRNA. This also inhibited miR-582-3p binding m6A nearby region. In addition,...
N-methyl-d-aspartate receptors (NMDARs), key excitatory ion channels, have gained attention as anti-depression targets. NMDARs consist of two GluN1 and GluN2 subunits (2A-2D), which determine their pharmacological properties. Few compounds selectively targeting with antidepressant effects been identified. Here, we present YY-23, a compound that inhibits GluN2C- or GluN2D-containing NMDARs. Cryo-EM analysis revealed YY-23 binds to the transmembrane domain GluN2D subunit. primarily affects on...
Anaplastic thyroid carcinoma (ATC) is the most lethal malignancy without a reliable therapeutic agent. Resveratrol possesses cancer-suppressive effects, while its effect(s) on ATC cells remains unknown. Because oxidative damage caused by increased reactive oxygen species (ROS) one of effects anticancer drugs and stress-caused mitochondria swelling observed in resveratrol-treated cancer cells, statuses their relevance with resveratrol sensitivities are elucidated using THJ-16T THJ-11T...
Anaplastic thyroid cancer (ATC) is a highly lethal undifferentiated malignancy without reliable therapies. Retinoic acid (RA) has been employed to promote redifferentiation of cancers by increasing their I131 uptake and radio-sensitivity, but its effect(s) on ATCs not yet ascertained. Likewise, resveratrol induces but, also in this case, effects remain unknown. These issues have addresses the current study using three human ATC cell lines (THJ-11T, THJ-16T, THJ-21T) through multiple...
Non-small cell lung cancer (NSCLC) is the most common pathological type of LC and ranks as leading cause deaths. Circulating exosomes have emerged a valuable biomarker for diagnosis NSCLC, while performance current electrochemical assays exosome detection constrained by unsatisfactory sensitivity specificity. Here we integrated ratiometric biosensor with an OR logic gate to form assay surface protein profiling from clinical serum samples. By using specific aptamers recognition clinically...
Yes-associated protein (YAP) activation is crucial for tumor formation and development, its stability regulated by ubiquitination. ISGylation a type of ubiquitination like post-translational modification, whereas whether YAP ISGylated how influences ubiquitination-related function remains uncovered. In addition, can activate glucose metabolism activating the hexosamine biosynthesis pathway (HBP) glycolysis, generate large number intermediates to promote proliferation. However, stimulates...
In this study, a really simple and efficient catalytic protocol for the construction of quinazolines from alcohol diamine has been developed based on CuCoAl layered double hydroxide (CuCoAl-LDH). The CuCoAl-LDH catalyst could accelerate cascade reactions without any additives tolerate various alcohols with satisfactory yields. Cooperation between Cu
Abstract The poor bioavailability of mangiferin (MGF) is a major obstacle on its further development. Aimed to illustrate the underlying mechanism and improve exposure, compared PK profiles MGF norathyriol (NTR) after different preparation were performed: pure MGF, Rhizoma Anemarrhenae (Zhi‐mu) decoction, timosaponin B2 (TB‐2) combination. Furthermore, potential contributing factors, including uridine diphosphoglucuronosyltransferase (UGT), cytochrome P450 (CYP450), P‐gp, enterobacterial...