A5102723348- Acute Myeloid Leukemia Research
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Wnt/β-catenin signaling in development and cancer
- RNA modifications and cancer
- Axon Guidance and Neuronal Signaling
- Glycosylation and Glycoproteins Research
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Extracellular vesicles in disease
- Chronic Myeloid Leukemia Treatments
- Histone Deacetylase Inhibitors Research
- Pluripotent Stem Cells Research
- Fibroblast Growth Factor Research
- Renal and related cancers
- Autophagy in Disease and Therapy
- Kruppel-like factors research
- Galectins and Cancer Biology
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Signaling Pathways in Disease
Affiliated Hospital of Jiangsu University
2014-2022
Jiangsu University
2014-2022
Shanghai Clinical Research Center
2018-2022
Zhanjiang Experimental Station
2022
Chemoresistance is one of the causes associated with poor prognosis in gastric cancer. MicroRNAs (miRNAs) are important regulators chemoresistance. Exosome-mediated delivery anti-cancer molecules and drugs have emerged as a new approach for cancer therapy. We first examined expression miR-374a-5p serum by qRT-PCR explored clinicopathological parameters. then performed vitro cell molecular studies, including CCK-8 assay, flow cytometry, qRT-PCR, western blot, to determine roles...
Cancer-testis (CT) antigens, rarely in normal tissues except testis, are expressed many tumor types. In recent years, DDX43 has been shown to be several malignancies. However, the role of during tumorigenesis is not well established. present study, we explored function chronic myeloid leukemia (CML). We found that overexpression CML cell lines enhanced survival and colony formation, inhibited apoptosis, promoted tumorigenesis, progression. contrast, silencing tumorigenesis. Upregulated H19...
MicroRNA-186 (miR-186) plays an important role in the pathogenesis of several cancers. Our study was intended to investigate expression status and prognostic implication miR-186 acute myeloid leukemia (AML).Real-time quantitative PCR carried out 112 de novo AML patients 28 controls.The level significantly down-regulated compared normal controls (p < 0.001). Patients with low presented older age than those high = 0.004). MiR-186high had a higher frequency CEBPA mutation miR-186low (20% 4%,...
Abstract Background Obesity confers enhanced risk for multiple diseases including cancer. The DNA methylation alterations in obesity-related genes have been implicated several human solid tumors. However, the underlying role and clinical implication of acute myeloid leukemia (AML) has yet to be elucidated. Results In discovery stage, we identified that methylation-associated LEP expression was correlated with prognosis among from databases Cancer Genome Atlas. validation verified...
Abstract The clinical activity of decitabine (5‐aza‐2‐deoxycytidine, DAC), a hypomethylating agent, has been demonstrated in acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) patients. However, secondary resistance to this agent often occurs during treatment leads failure. It is important clarify the mechanisms underlying for improving efficacy. In study, by gradually increasing concentration after continuous induction DAC, we established DAC‐resistant K562 cell line (K562/DAC)...
MicroRNA-29c (miR-29c) is abnormally expressed in several cancers and serves as an important predictor of tumor prognosis. Herein, we investigate the effects abnormal miR-29c expression analyze its clinical significance acute myeloid leukemia (AML) patients. In addition, decitabine (DAC) has made great progress treatment AML recent years, but DAC resistance still common phenomenon mechanism unclear. We further influences to leukemic cells treated with DAC.Real-time quantitative PCR (RQ-PCR)...
Acute myeloid leukemia (AML) is the most common type of hematological malignancy. Recently, an increasing number reports have shown that many circular RNAs can act as effective targets for AML. However, roles circ_0059707 in AML remain largely unclear. In this study, we found expression levels were significantly decreased patients with respect to normal controls (p < 0.001). Low also associated a poor prognosis. Furthermore, overexpression inhibited cell growth and promoted apoptosis...
Abstract Background Previously, we reported the expression of DLX4 isoforms ( BP1 and DLX7 ) in myeloid leukemia, but functional role remains poorly understood. In work described herein, further determined underlying chronic leukemia (CML) leukemogenesis. Methods The methylation were detected by real-time quantitative PCR (RT-qPCR) methylation-specific (RT-qMSP) patients with CML. was vitro vivo. molecular mechanism leukemogenesis identified based on chromatin immunoprecipitation...
Background: Various circular RNA (circRNA) molecules are abnormally expressed in acute myeloid leukemia (AML), and associated with disease occurrence development, as well patient prognosis. The roles of circ_0059706, a circRNA derived from ID1, AML remain largely unclear. Results: Here, we reported circ_0059706 expression de novo its association We found that was significantly lower patients than controls (p < 0.001). Survival analysis divided into two groups according to high low showed...
RNA N6-methyladenosine (m6A) is the most common and intensively studied modification that critically regulates metabolism, cell signaling, survival, differentiation. However, overall role of multiple m6A regulators in tumor microenvironment (TME) has not yet been fully elucidated acute myeloid leukemia (AML). In our study, we explored genetic transcriptional alterations 23 AML patients. Three distinct molecular subtypes were identified associated with prognosis, patient clinicopathological...
Background: Leukemia stem cell (LSC)-enriched genes have been shown to be highly prognostic in acute myeloid leukemia (AML). However, the value of tumor suppressor LSC remains largely unknown.
An immortalized mouse fetal liver stromal cell line, named KM3, has demonstrated the potential to support growth and maintenance of human embryonic stem cells (hESCs). In this study, characteristics KM3 were examined following cryopreservation at -70°C in liquid nitrogen for 15, 30 60 days treatment with 10 μg/ml mitomycin C. addition, whether suitable use as feeder hESCs was evaluated. The inhibition mitosis without death observed when treated C 2 h. morphology cryopreserved not markedly...
Epigenetic dysregulation of cancer-associated genes has been identified to contribute the pathogenesis myelodysplastic syndromes (MDS). However, few studies have elucidated whole-genome DNA methylation in initiation MDS. Reduced representation bisulfite sequencing was performed five de novo MDS patients and four controls investigate epigenetic alterations pathogenesis. The mean global showed no significant difference compared with controls. In depth, a total 1,459 differentially methylated...
To investigate the methylation status of miR-378 promoter in chronic myeloid leukemia (CML) and to analyze its clinical significance.The unmethylation level gene bone marrow mononuclear cells 25 healthy donors 53 patients with CML was detected by using real-time quantitative methylation-specific PCR (RQ-MSP).The hypomethylation found 17/53 (32.1%) patients, but only 1/25 (4.0%) controls. The difference between two groups very statistically significant (P < 0.01). frequency at phase (CP),...
Abstract RNA N6-methyladenosine (m6A) is the most common and intensively studied modification that critically regulates metabolism, cell signaling, survival, differentiation. However, overall role of multiple m6A regulators in tumor microenvironment (TME) has not yet been fully elucidated acute myeloid leukemia (AML). In our study, we explored genetic transcriptional alterations 23 AML patients. Three distinct molecular subtypes were identified associated with prognosis, patient...