A5027222300- Angiogenesis and VEGF in Cancer
- Endometriosis Research and Treatment
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Folate and B Vitamins Research
- Milk Quality and Mastitis in Dairy Cows
- Vitamin D Research Studies
- Platelet Disorders and Treatments
- Nuclear Structure and Function
- Gestational Diabetes Research and Management
- Transplantation: Methods and Outcomes
- Skin and Cellular Biology Research
- T-cell and Retrovirus Studies
- Cardiovascular Disease and Adiposity
- Reproductive tract infections research
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Facial Trauma and Fracture Management
- Animal Disease Management and Epidemiology
- Rabbits: Nutrition, Reproduction, Health
- Selenium in Biological Systems
- Maternal Mental Health During Pregnancy and Postpartum
- Atherosclerosis and Cardiovascular Diseases
- Aquaculture disease management and microbiota
UNSW Sydney
1989-2024
Sutherland Hospital
2019-2024
St George Hospital
2006-2012
St George's Hospital
2007
St. George Hospital
2006
Lions Eye Institute
1999
Prince of Wales Hospital
1991-1997
University of Maryland, Baltimore
1994
Biotechnology Institute
1994
Pennsylvania State University
1987-1994
ABSTRACT Objective To identify a microbial signature for endometriosis use as diagnostic non‐invasive biomarker. Design Prospective cohort pilot study. Setting Nepean Hospital and UNSW Microbiome Research Centre, Australia. Population Sixty‐four age‐ sex‐matched subjects ( n = 19 healthy control (HC); 24 non‐endometriosis (N‐ENDO) 21 confirmed (ENDO)). All study participants, besides controls, underwent laparoscopic surgical assessment endometriosis, histology was performed on excised...
Previous work with cultured mammalian cells and perfused laboratory animals suggested to us that hydrolysis of homocysteine thiolactone was catalyzed in these systems. We confirmed this finding by measuring the sulfhydryl-releasing activity endothelial from human umbilical arteries solution, pH 7.4, 37 degrees C. The reaction vigorous stereospecific showed saturation kinetics (Km values for L- D,L-homocysteine were 3.9 8.2 mmol/l, respectively, Vmax 10.75 10.1 mumol/min/10(9) cells,...
Alteration of the gut virome has been associated with colorectal cancer (CRC); however, when and how alteration takes place not studied. Here, we employ a longitudinal study in mice to characterize azoxymethane (AOM)-induced neoplasia identify important viruses tumor growth. The number size tumors increased as aged AOM treated group, compared control group. Tumors were first observed group at week 12. We significantly lower alpha diversity shift viral profile appeared. In addition,...
Platelet-derived growth factor (PDGF) is a potent chemotactic and mitogenic implicated to play important roles in variety of normal pathophysiologic settings. We investigated PDGF receptor expression on human megakaryocytes several megakaryocytic cell lines (CHRF, DAMI, Meg-01, M-07e) using enzyme-linked immunosorbent assay (ELISA), flow cytometry immunocytochemical staining. Both subtypes were identified CHRF, Meg-01 cells by ELISA; beta-receptor levels exceeded alpha-receptor levels. Flow...
Summary Histories of 4 dairy herds with increased incidence Pseudomonas mastitis associated contaminated wash hoses in milking parlors are described. Problems detection and elimination the organism from water sources inadequacy iodide germicides eliminating discussed. In problem herds, greater numbers organisms often found left standing between milkings. Thus, flushing before their use process may be beneficial reducing exposure cows to organism.
The effects of bovine leukosis virus (BLV) on the phenotypic and functional characteristics peripheral blood mononuclear cells were investigated. Whole differentials showed that persistently lymphocytotic (BLV+PL) dairy cattle had more lymphocytes fewer neutrophils than aleukemic seropositive (BLV+AL) or seronegative (BLV–) animals. Flow cytometric analyses indicated BLV+PL animals B lymphocytes, with a concomitant decrease in CD2 positive when compared BLV– group. Mononuclear from BLV+AL...
The mechanism by which nuclear and cytoplasmic filaments are sorted in vivo was studied examining lamin sequences required to target an otherwise IF protein, the small neurofilament subunit (NF-L), lamina. By swapping corresponding domains between NF-L A, envelope targeting of shown require presence "head" domain, a 42-amino acid sequence unique rod domains, localization signal CAAX motif. Replacement entire COOH-terminal tail A with that had no discernible effect on provided substituted...
Summary This study investigated in vitro biological response modifiers (BRM) possibly involved initiation and regulation of human capillary endothelial cell (HCEC) growth. An indicator assay was developed using tritiated thymidine to measure increased DNA turnover. Purified or recombinant BRMs tested singly combination included: interferon (IFN; α γ), tumour necrosis factor (TNF; β), transforming growth (TGF: β). rIL‐1, rIL‐2, pIL‐2, platelet derived (PDGF) retinoic acid. Under limiting...
Objective To identify a diagnostic microbial signature for endometriosis. Design Prospective cohort study Setting Nepean Hospital and UNSW Microbiome Research Centre, St George Hospital, Australia Population 64 age- sex-matched subjects ( n=1 9 HC; n= 24 N-ENDO 21 ENDO). All participants, besides healthy controls, underwent laparoscopic surgical assessment endometriosis, histology was performed on excised lesions. Methods Oral, stool, vaginal samples were self-collected at single time point...
To develop a poly(2-hydroxyethyl methacrylate) orbital implant that allows tissue ingrowth and direct muscle attachment to minimize the risk of extrusion enhance cosmesis.Assessment clinical outcomes histologic findings after implantation 18 prototype prostheses into rabbits. The implants were not wrapped with other tissues or materials.One case infection was observed but there no extrusions, up 21 months follow-up. Biocolonization confirmed histologically. Good movement when cosmetic shell...
Introduction Vaginal estrogen is a treatment for genitourinary symptoms of menopause (GSM), which comprises vaginal atrophy and urinary dysfunction, including incontinence. Previous studies show that therapy promotes lactobacilli abundance associated with reduced GSM symptoms, reduction stress However, detailed longitudinal characterize how the microbiome changes in response to are scarce. We aimed compare microbiota postmenopausal women, before 12 weeks after cream. Methods A total 44...
The microbiome has emerged as a key determinant of human health and reproduction, with recent evidence suggesting dysbiotic is implicated in adverse perinatal outcomes. existing research been limited by the sample collection timing, cohort design, lack data on preconception microbiome. This prospective, longitudinal study will recruit 2000 Australian women, order to fully explore role development Participants are enrolled for maximum 7 years, from 1 year preconception, through 5 years...
This study aimed to determine the optimal growth factor combination for expansion of megakaryocyte (Mk) progenitors with clonogenic potential from CD34+-enriched mobilized peripheral blood stem cells (PBSC). Mobilized PBSC were monocyte depleted and CD34+ enriched, then cultured various combinations interleukin-3 (IL-3), IL-6, IL-11, Flt3 ligand (Flt3-L), cell (SCF), granulocyte-macrophage colonystimulating (GM-CSF), erythropoietin (EPO), using a 27-3 IV fractional factorial design....
Summary The effects of selenium (Se) status on the distribution and functional capabilities bovine peripheral blood mononuclear cells (PBMC) were examined. No differences in proliferative responses to mitogens, interleukin (IL)‐2 production, IL‐2 receptor expression, or lymphocyte trafficking patterns observed between PBMC from dairy cows maintained Se adequate deficient diets. In contrast, deficiency significantly reduced numbers circulating monocytes when compared fed a diet. Differences...
In a field study of 29 dairy farms, Pseudomonas aeruginosa was isolated more frequently (P = 0.05) from milking parlor udder wash water systems containing iodophor germicides than those with no germicide. Most available iodine (AI2) concentrations were below the recommended level 25 ppm (25 microgram/ml). Rubber and polyvinyl chloride hoses caused rapid decreases in AI2 solutions. dropped to 6 or less 240 min for solutions contained either rubber, compared glass, which unchanged min....