A5090700538- Prostate Cancer Treatment and Research
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Urinary Tract Infections Management
- Pediatric Urology and Nephrology Studies
- Histone Deacetylase Inhibitors Research
- Hippo pathway signaling and YAP/TAZ
- Glycosylation and Glycoproteins Research
- Clostridium difficile and Clostridium perfringens research
- Protein Degradation and Inhibitors
- Urinary Bladder and Prostate Research
- Peptidase Inhibition and Analysis
- COVID-19 Clinical Research Studies
- Eosinophilic Disorders and Syndromes
- Immunotherapy and Immune Responses
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Infective Endocarditis Diagnosis and Management
- COVID-19 and healthcare impacts
- FOXO transcription factor regulation
- Food Safety and Hygiene
- COVID-19 and Mental Health
- Wnt/β-catenin signaling in development and cancer
- Infection Control and Ventilation
Newcastle University
2012-2024
University of Maryland, Baltimore
2021-2023
VA Maryland Health Care System
2021-2023
New York Proton Center
2022-2023
Communities In Schools of Orange County
2022-2023
Manhattan Institute for Policy Research
2023
Oregon Medical Research Center
2022-2023
Lindsay Unified School District
2022-2023
Ann Arbor Center for Independent Living
2022
University of Richmond
2022
Tip60 (KAT5) is a histone acetyltransferase (HAT enzyme) involved in multiple cellular processes including transcriptional regulation, DNA damage repair and cell signalling. In prostate cancer, aggressive cases over-express which functions as an androgen receptor co-activator via direct acetylation of lysine residues within the KLKK motif hinge region. The purpose this study was to identify characterise acetylase inhibitor. High-throughput screening revealed isothiazole that inhibited both...
The androgen receptor (AR) is a key molecule involved in prostate cancer (PC) development and progression. Post-translational modification of the AR by co-regulator proteins can modulate its transcriptional activity. To identify which demethylases might be regulation, an siRNA screen was performed to reveal that demethylase, KDM4B, may important protein. KDM4B enzymatic activity required enhance activity; however, independently this activity, protein stability via inhibition ubiquitination....
Abstract Immune checkpoint blockade has yet to produce robust anti-cancer responses for prostate cancer. Sialyltransferases have been shown across several solid tumours, including breast, melanoma, colorectal and promote immune suppression by synthesising sialoglycans, which act as ligands Siglec receptors. We report that ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) levels negatively correlate with androgen signalling in tumours. demonstrate ST3Gal1 plays an important role...
Endocrine therapy has successfully been used to treat estrogen receptor (ER)-positive breast cancer, but this invariably fails with cancers becoming refractory treatment. Emerging evidence suggested that fluctuations in ER co-regulatory protein expression may facilitate resistance and be involved cancer progression. To date, a small number of enzymes control methylation status histones have identified as co-regulators signalling. We the histone H3 lysine 9 mono- di-methyl demethylase enzyme...
The importance of the estrogen receptor (ER) in breast cancer (BCa) development makes it a prominent target for therapy. Current treatments, however, have limited effectiveness, and hence definition new therapeutic targets is vital. ER member nuclear hormone superfamily transcription factors that requires co-regulator proteins complete regulation. Emerging evidence has implicated small number histone methyltransferase (HMT) demethylase (HDM) enzymes as regulators signalling, including H3...
Hospitalized patients with SARS-CoV-2 infection (COVID-19) often receive antibiotics for suspected bacterial coinfection. We estimated the incidence of coinfection and secondary in COVID-19 using clinical diagnoses to determine how frequently are administered when is absent. performed a retrospective cohort study inpatients present on admission hospitals Premier Healthcare Database between April June 2020. Bacterial infections were defined ICD-10-CM diagnosis codes associated "present...
Urine cultures are nonspecific and often lead to misdiagnosis of urinary tract infection unnecessary antibiotics. Diagnostic stewardship is a set procedures that modifies test ordering, processing, reporting in order optimize diagnosis downstream treatment. In this study, we aimed develop expert guidance on best practices for urine culture diagnostic stewardship.A RAND-modified Delphi approach with multidisciplinary panel was used ascertain practices. Clinical questions guide recommendations...
<h3>Background</h3> Germline mutations in <i>CDH1</i> are associated with hereditary diffuse gastric cancer; lobular breast cancer also occurs excessively families such condition. <h3>Method</h3> To determine if is a susceptibility gene for women without family history of cancer, germline DNA was analysed the presence 318 who were diagnosed before age 45 years or had and not known, known not, to be carriers <i>BRCA1</i> <i>BRCA2</i>. Cases ascertained through registries high-risk genetic...
Coronavirus disease (COVID)-related misinformation is prevalent online, including on social media. The purpose of this study was to explore factors associated with user engagement COVID-related the media platform, TikTok. A sample TikTok videos hashtag #coronavirus downloaded September 20, 2020. Misinformation evaluated a scale (low, medium, and high) using codebook developed by experts in infectious diseases. Multivariable modeling used evaluate number views presence comments indicating...
Abstract The recently updated Society for Healthcare Epidemiology of America, the Infectious Diseases and Association Professionals in Infection Control practice recommendations methicillin-resistant Staphylococcus aureus (MRSA) prevention acute care facilities list contact precautions (CPs) patients known to be infected or colonized with MRSA as an “essential practice,” meaning that it should adopted all facilities. We argue existing evidence on benefits harms associated CP do not justify...
Abstract Resistance to androgen receptor (AR) targeting therapeutics in prostate cancer (PC) is a significant clinical problem. Mechanisms by which this accomplished include AR amplification and expression of splice variants, demonstrating that remains key therapeutic target advanced disease. For the first time we show IKBKE drives signalling PC. Significant inhibition regulated gene was observed upon siRNA-mediated depletion or pharmacological due inhibited multiple cell line models...
This SHEA white paper identifies knowledge gaps and challenges in healthcare epidemiology research related to coronavirus disease 2019 (COVID-19) with a focus on core principles of epidemiology. These gaps, revealed during the worst phases COVID-19 pandemic, are described 10 sections: epidemiology, outbreak investigation, surveillance, isolation precaution practices, personal protective equipment (PPE), environmental contamination disinfection, drug supply shortages, antimicrobial...
// Urszula L. McClurg 1 , Victoria J. Harle Arash Nabbi 2 Amanda Batalha-Pereira Scott Walker Kelly Coffey Luke Gaughan Stuart RC McCracken and Craig N. Robson Solid Tumour Target Discovery Laboratory, Newcastle Cancer Centre, Northern Institute for Research, Medical School, University, upon Tyne, United Kingdom Department of Biochemistry Molecular Biology, Southern Alberta Research Institute, Cumming School Medicine, University Calgary, AB, Canada Correspondence to: Robson, email: Keywords...
Stem cells are thought to be the cell of origin in malignant transformation many tissues, but their role human prostate carcinogenesis continues debated. One conflicts with this model is that cancer stem have been described lack androgen receptor (AR) expression, which established importance initiation and progression. We re-examined expression patterns AR within adult epithelial differentiation using an optimised sensitive specific approach examining transcript, protein regulated gene...
This unit provides information how to use short interfering RNA (siRNA) for sequence specific gene silencing in mammalian cells. Several ways siRNA generation and optimisation, as well recommendations cell transfection are presented.
Microscopic diagnosis of malaria using Giemsa-stained blood smears is the standard care in resource-limited settings. These represent a potential source DNA for PCR testing to confirm Plasmodium infections or epidemiological studies archived samples. Therefore, we assessed use extracts from stained detection species real-time PCR. We extracted and corresponding red cell pellets collected asymptomatic children southwestern Uganda 2010. then performed followed by high-resolution melting (HRM)...
Abstract Background Anaplasmosis presents with fever, headache, and laboratory abnormalities including leukopenia thrombocytopenia. Polymerase chain reaction (PCR) is the preferred diagnostic but overutilized. We determined if routine tests could exclude anaplasmosis, improving PCR utilization. Methods Anaplasma results from a 3-year period, associated complete blood count (CBC) liver function test results, were retrospectively reviewed. rejection criteria, based on white cell (WBC) platelet...
Granulicatella adiacens is a Gram-positive coccus, formerly grouped with nutritionally variant Streptococcus , often found as commensal bacteria of the human oral cavity, urogenital tract, and gastrointestinal tract. Prior case reports have demonstrated spp. pathogen that can cause bacteremia infective endocarditis particularly prosthetic valves pacemaker leads. Here, we report on unique bacterascites in 50-year-old male.
A 53-year-old man with hypertrophic obstructive cardiomyopathy was evaluated for cough that had developed 3 months earlier, shortly after he returned from extensive international travel. Laboratory evaluation revealed an absolute eosinophil count of 3800 to 4550 per cubic millimeter. Additional diagnostic tests were performed.
The methyltransferase, KMT5A has been proposed as an oncogene in prostate cancer and therefore represents a putative therapeutic target. To confirm this hypothesis we have performed microarray study cell line model of androgen independence following knockdown the presence transcriptionally active receptor (AR) to understand which genes cellular processes are regulated by AR. We observed that 301 were down-regulated whilst 408 up-regulated when expression was reduced. KEGG pathway Gene...