A5044300856- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Cardiac Ischemia and Reperfusion
- Nitric Oxide and Endothelin Effects
- Renal and related cancers
- Cardiovascular Function and Risk Factors
- Circular RNAs in diseases
- Anesthesia and Neurotoxicity Research
- Fuel Cells and Related Materials
- Extracellular vesicles in disease
- Renal and Vascular Pathologies
- Heart Failure Treatment and Management
- Cardiac Arrest and Resuscitation
- Pregnancy and preeclampsia studies
- RNA regulation and disease
- Hormonal Regulation and Hypertension
- GDF15 and Related Biomarkers
- Acute Kidney Injury Research
- Neurofibromatosis and Schwannoma Cases
- Renal cell carcinoma treatment
- Cardiac electrophysiology and arrhythmias
- Pediatric Urology and Nephrology Studies
- Dialysis and Renal Disease Management
- Chromosomal and Genetic Variations
- Renin-Angiotensin System Studies
Medical College of Wisconsin
2013-2025
Augusta University Health
2025
RELX Group (United States)
2019
Johns Hopkins University
2016
American Society of Anesthesiologists
2015
Zero to Three
2015
Novem (Netherlands)
2015
The role of microRNA-21 in isoflurane-induced cardioprotection is unknown. authors addressed this issue by using knockout mice and explored the underlying mechanisms.C57BL/6 were echocardiographically examined. Mouse hearts underwent 30 min ischemia followed 2 h reperfusion vivo or ex presence absence 1.0 minimum alveolar concentration isoflurane administered before ischemia. Cardiac Akt, endothelial nitric oxide synthase (eNOS), neuronal (nNOS) proteins determined Western blot analysis....
Diabetic heart disease is associated with tetrahydrobiopterin oxidation and high arginase activity, leading to endothelial nitric oxide synthase dysfunction. Sepiapterin (SEP) a precursor, L-citrulline (L-Cit) converted substrate, L-arginine. Whether SEP L-Cit are effective at reducing diabetic not known. The present study examined the effects of on cardiomyopathy ischemia/reperfusion injury in obese type 2 mice.Db/db C57BLKS/J mice 6 8 weeks age received vehicle, SEP, or orally alone...
Ischemia reperfusion (I/R) injury is an important initiating cause of chronic kidney disease and renal failure. Changes in proximal tubule (PT) morphology, including brush border loss, occur rapidly response to ischemic stress I/R injury. Vacuole membrane protein 1 (VMP1) a compelling target for ischemia-associated damage, because it necessary regulator autophagy the genomic location hypoxia-responsive microRNA miR-21 lies within intronic region Vmp1 gene. Autophagy reported have protective...
Atrial natriuretic peptide (ANP), encoded by Nppa, is a vasodilatory hormone that promotes salt excretion. Genome-wide association studies identified Nppa as causative factor of blood pressure development, and in humans, ANP levels were suggested an indicator sensitivity. This study aimed to provide insights into the effects on cardiorenal function salt-sensitive hypertension. To address this question, hypertension was induced SSNPPA-/- (KO Dahl [SS] rat background) or SSWT (WT SS) rats...
Abstract Aims Previous studies indicate that nitric oxide derived from endothelial synthase (eNOS) serves as both trigger and mediator in anaesthetic cardiac preconditioning. The mechanisms underlying regulation of eNOS by volatile anaesthetics have not been fully understood. Therefore, this study examined the role vascular growth factor (VEGF) isoflurane Methods results Wistar rats underwent 30 min coronary artery occlusion followed 2 h reperfusion. Isoflurane given prior to...
Abstract Diabetic cardiomyopathy increases the risk of heart failure and death. At present, there are no effective approaches to preventing its development in clinic. Here we report that reduction cardiac GTP cyclohydrolase 1 (GCH1) degradation by genetic pharmacological protects against diabetic cardiomyopathy. was induced C57BL/6 wild-type mice transgenic with cardiomyocyte-specific overexpression GCH1 streptozotocin control animals were given citrate buffer. We found diabetes-induced...
The 5/6 nephrectomy (5/6Nx) rat model recapitulates many elements of human CKD. Within weeks surgery, 5/6Nx rats spontaneously exhibit proximal tubular damage, including the production very large extracellular vesicles and brush border shedding. We hypothesized that elimination these structures, termed renal (LRT-EVs), into urine represents a pathologic mechanism by which essential tubule proteins are lost.LRT-EVs were isolated from 10 after surgery. LRT-EV diameters measured. proteomic...
Background: Emerging evidence suggests that GTP cyclohydrolase 1 (GTPCH) is critical for activity of nitric oxide synthase and sarcoplasmic reticulum Ca 2+ ATPase myocardial relaxation in normal myocardium. We hypothesized increased expression GTPCH ameliorates cardiac dysfunction streptozotocin (STZ)-induced diabetic cardiomyopathy. Methods: Transgenic mice with cardiomyocyte-specific overexpression human gene (GTPCH-Tg) their wild-type littermates were injected 50 mg/kg/day STZ (i.p.) 5...
Chronic kidney disease (CKD) mediates pathological changes in heart and vascular tissues, yet many molecular mechanisms by which renal injury directly affects cardiovascular pathology (CVD) are unknown. miRNAs proposed to be powerful regulators of pathophysiological signaling networks; having the potential radically change landscape mRNA transcript expression protein abundance context health disease. We have profiled a rat model CKD – 5/6 nephrectomy (5/6Nx) with objective identify strong...
Type 4 cardiorenal syndrome (CRS4) is a complex disorder in which primary chronic kidney disease (CKD) contributes to secondary cardiovascular (CVD). CKD patients experience significant increase relative risk of cardiac events and premature mortality compared the general population. While link between heart well‐established, mechanisms by mediates CVD are not well understood. microRNA (miR) research rapidly growing area as miRs have been shown be important mediators pathophysiology. We...
Severe acute kidney injury (AKI) can lead to residual tubular damage and chronic disease. Proximal convoluted tubule cells produce apical blebs in response ischemic stress. Blebs are shed into the lumen, resulting loss of membrane transporters. Hypoxia regulated microRNA miR‐21 is well known be rapidly upregulated hypoxia or ischemia. The genetic sequence lies within an intronic region vacuole protein 1 ( Vmp1) , a gene which encodes transmembrane involved with autophagy. While each...
Atrial natriuretic peptide (ANP), encoded by the Nppa gene, is an osmoregulatory hormone that promotes salt excretion. GWAS identified to be associated with hypertension; some studies suggest varying ANP levels as indicator of salt-sensitivity. The aim current project was assess effects deficiency on cardiac damage and function using a knockout in Dahl Salt-Sensitive (SS) rat background (SS NPPA-/- ). A combination vivo techniques ex biochemical methods were used test role development SS...
Type 4 cardiorenal syndrome is a complex disorder in which primary chronic kidney disease (CKD) contributes to secondary cardiovascular (CVD). Although the pathological link between and heart are well-characterized, molecular switches drive cardiac pathology not well understood. miRNAs have potential be master regulators of cellular signaling networks through modulation RNA protein expression profiles been shown important both health disease. Previous work our laboratory identified...
The mechanisms that regulate renal survival and atrophy under pathological stress remain incompletely understood. This knowledge is essential for developing new strategies to preserve function in patients with various forms of disease. We have utilized the 5/6 nephrectomy (5/6Nx) model study effects insufficiency 10 week old male Sprague Dawley rats. In this excision 2/3 left kidney entire right induced substantial hypertrophy remnant within 7 weeks surgery. Conversely, kidney, while leaving...
Previous studies in our laboratory have shown that the 5/6 nephrectomy model of CKD a pathological upregulation miR‐21‐5p occurs left ventricle, which suppresses peroxisome proliferator‐activated receptor alpha and impacts regulation fatty acid oxidation glycolysis. Subsequent H9C2 (cardiomyoblast, ATCC) cell showed overexpression reduced lipid content peroxidation response to treatment culture. Studies oxygen consumption rate (OCR) induced shift cellular metabolism toward reliance on...
Adam, Ryan J.; Paterson, Mark; Hoffmann, Brian R.; Kriegel, Alison J. Author Information
Chronic kidney disease (CKD) progression differs in males and females the influence of gonadal hormones on pathology are incompletely understood. We utilized 5/6 nephrectomy (5/6Nx) rat model, which recapitulates hallmarks human CKD, to study differences regulation CKD between sexes. previously found that genomic deletion microRNA 146b-5p (miR-146b) results a sex-dependent phenotype 5/6Nx rats. Male miR-146b-/- rats develop renal similar their WT counterparts. However, female experience...