The emergence of drug-resistant bacteria is a severe problem in aquaculture. ability drug resistance genes to transfer from bacterial cell another thought be responsible for the wide dissemination these aquaculture environment; however, little known about gene mechanisms marine bacteria. In this study, we show that tetracycline-resistant strain Photobacterium damselae subsp. damselae, isolated seawater at coastal site Japan, harbors novel multiple plasmid. This plasmid named pAQU1 can...

The topical corticosteroid phobia (TOPICOP) score is a useful tool for evaluating the severity of steroid phobia. Following development novel therapies, such as delgocitinib, Janus kinase inhibitor, treatment options atopic dermatitis (AD) have significantly expanded. Herein, we report case 6-year-old girl with moderate AD whose was complicated by her mother's (TOPICOP score: 52.7). Despite detailed explanations addressing misconceptions regarding corticosteroids (TCS), mother refused its...

T his study was undertaken in an effort to understand the role of cytokines on human gingival fibroblasts and lymphocyte trafficing into inflamed tissue. Using flow cytometry we examined determine level cell surface expression percentage cells positive for intercellular adhesion molecule 1 (ICAM‐1), HLA‐DR antigen, function‐associated antigen 3 (LFA‐3), CD44 molecule, which are involved presentation. The following were used: interleukin‐1β (IL‐1β), tumor necrosis factor‐α (TNF‐γ),...

Little is known about the association between maternal use of heated tobacco products (HTPs) during pregnancy and onset allergy among offspring. This study aimed to determine whether HTP smoking associated with in their offspring evaluate potential dose-response association.

We isolated and characterized an immunogenic protein of oral spirochete, Treponema denticola Johnson. A genomic DNA library constructed with bacteriophage lambda EMBL3 as a vector was immunologically screened rabbit antiserum against the whole cells. Using Western immunoblot analysis, we found particular clone encoding antigen molecular weight 53,000; designated T. (TdpA). Complete sequence determination revealed open reading frame 1,419 bp signal peptide that homologous to bacterial...

Component-resolved diagnostics is used to diagnose food allergies. However, few reports have evaluated the severity of peach fruit allergy using allergen components, including Pru p 7.This study aimed predict based on presence specific IgE (sIgE) antibodies (Abs) allergenic components.Twenty-seven patients with were enrolled and classified into two groups: local reaction (LR) group, 12 only oral or throat mucosal symptoms, systemic (SR) 15 patients, 10 whom experienced anaphylaxis. Serum...

Porphyromonas gingivalis, a putative pathogen in human periodontal disease, possesses 60-kDa heat shock protein (hsp60, GroEL). The GroEL homologs are known to be key molecules auto-immune reactions because of the sequence similarity with hsp60. In this study, B-cell epitopes on P. gingivalis (PgGroEL) were analyzed by both Western immunoblotting truncated PgGroEL and multi-pin synthetic peptide approach. To examine auto-antibody production periodontitis patients, gingival fibroblasts was...

Breath sound parameters have been suggested as biomarkers of the airway narrowing in children. Using a commercially available breath analyzer, characteristics condition were investigated infants with risk factors for asthma development.A total 443 (mean age, 9.9 months; range, 3-24 months) included present study. The frequency limiting 99% power spectrum (F99), roll-off from 600 to 1200 Hz (Slope) and curve indices, area under dBm data (A3/AT) ratio at 50% 75% highest (RPF75 RPF50),...

ABSTRACT Subtractive hybridization was employed to isolate specific genes from virulent Porphyromonas gingivalis strains that are possibly related abscess formation. The genomic DNA the strain P. W83 subtracted with avirulent ATCC 33277. Three clones unique were isolated and sequenced. cloned fragments 885, 369, 132 bp had slight homology only Bacillus stearothermophilus IS 5377 , which is a putative transposase. regions flanking sequenced, gene structure around revealed. These three located...

Background: Vascular-type Ehlers-Danlos syndrome (vEDS) is caused by collagen III deficit resulting from heterogeneous mutations in COL3A1, which occasionally causes sudden death due to arterial/visceral rupture. However, it difficult conduct basic research on the pathophysiology of vEDS. Moreover, number patients with vEDS small, limiting available samples. Furthermore, symptoms may vary among family members, even if they share same mutation. Accordingly, many aspects pathology remain...

Over the past few decades, allergic diseases have become more prevalent and impact around 20% of global population. There is clinical significance march as it places a burden on quality life children their families.

Abstract Vascular‐type Ehlers–Danlos syndrome (vEDS) is an autosomal‐dominant inherited disorder caused by a deficit in collagen III. It results from heterogeneous mutations the α1 III gene ( COL3A1 ) and associated with life‐threatening complications, even younger patients. However, details of pathogenesis underlying mutation causing vEDS remain unclear. Here, we focus on anomalies fiber size endoplasmic reticulum (ER) stress response patients using electron microscopy (EM). We discovered...

Pollen food allergy syndrome (PFAS) is caused by cross-reactivity with pollen; however, not all-pollen-sensitised individuals develop PFAS, and studies on the characteristics of PFAS development are limited in Japan. We investigated prevalence risk factors for Japanese children adolescents sensitised to pollen their association pollen-specific IgE levels. The were patients allergies aged 3-18 years who visited Dokkyo Medical University Hospital between January 2016 December 2019. Specific...

Buckwheat is a rare causative food for protein–induced enterocolitis syndrome (FPIES). To date, it unknown what laboratory data patients with FPIES caused by buckwheat show. We report 4-year-old female and the results. Skin prick, specific IgE antibody, basophil activation tests were negative; however, lymphocyte stimulation test (LST) revealed 10.2-fold increase in compared negative control. In an open-label oral challenge (OFC) of 80 g boiled noodles, 3 hours after ingestion, vomiting...

Abstract A 4‐year‐old Japanese boy was referred to Osaka University Dental Hospital because of severe mobility and pain the right lower primary canine. The canine had bone loss a pocket depth exceeding 5‐6 mm. left showed slight moderate alveolar loss. other teeth no pathogenic findings. subgingival microflora from dominated by gram‐negative rods, especially capnocytophaga fusobacterium, while actinomyces sp. were most common gram‐positive bacteria. While neutrophil functions patient within...

Nerve growth factor (NGF), brain-derived neurotrophic (BDNF), and neurotrophin-3 (NT-3) are three representative neurotrophins responsible for the differentiation survival of neurons, their high-affinity receptors tropomyosin-receptor-kinase (TRK)A, TRKB, TRKC, respectively. In this study, we investigated expression in a mouse periodontal ligament cell line (MPL), by reverse transcription-polymerase chain-reaction (RT-PCR) enzyme-linked immunoabsorbent assay (ELISA). We also studied TRK on...

To evaluate the frequency of wheezing in infants, presence was examined normal infants using a breath sound analyzer, METHODS: A total 443 (age range, 3-24 months) were included present study. The existence audible and faint wheezing/inaudible wheezing-like noises (FW) confirmed on chest auscultation spectrogram. parameters spectrum, limiting 99% power spectrum (F99 ), roll-off from 600 to 1,200 Hz (slope) curve indices, area under dB data (A3 /AT B4 ratio at 50% 75% highest (RPF50 RPF75 )...

Abstract Background and objectives Using a lung sound analysis, the prognosis of asthma was investigated in infants with risk factors for development by long‐term observation. Methods A total 268 were included (median age: 8 months old). The parameters (the ratio third fourth area to under curve [A 3 /A T B 4 ], power frequency at 50% 75% highest [RPF 50 RPF 75 ]) evaluated first visit. At years old, using questionnaire, we examined relationship between development. Results Among infants,...

The gene encoding the crystalline surface layer (S-layer) protein from Campylobacter rectus, designated slp, was sequenced and recombinant product expressed in Escherichia coli. consisted of 4086 nucleotides a with 1361 amino acids. N-terminal acid sequence revealed that Slp did not contain signal sequence, but initial methionine residue processed. deduced displayed some common characteristic features S-layer proteins previously reported. A homology search showed high similarity to fetus...

Background: The parameters of lung sounds have been suggested as biomarkers airway changes. Using a commercially available sound analyzer, we investigated the characteristics in infants with acute respiratory infection (ARI). Methods: Infants ARI who were 6 to 18 months age included this study. parameters, ratio third area and fourth areas total under curve spectrum (A3/AT B4/AT), power frequency at 75% 50% highest (RPF75 RPF50) evaluated. With an original Japanese questionnaire based on...