A5026710237- Immune Cell Function and Interaction
- Circadian rhythm and melatonin
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- Photoreceptor and optogenetics research
- Electrochemical sensors and biosensors
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Resilience and Mental Health
- Light effects on plants
- Meningioma and schwannoma management
- Antimicrobial Peptides and Activities
- Silicone and Siloxane Chemistry
- Leadership, Courage, and Heroism Studies
- Immune Response and Inflammation
- Amino Acid Enzymes and Metabolism
- Hearing Impairment and Communication
- Advanced Polymer Synthesis and Characterization
- Educational Reforms and Innovations
- RNA and protein synthesis mechanisms
- Conducting polymers and applications
- RNA modifications and cancer
- Synthesis and properties of polymers
- Traumatic Brain Injury Research
- DNA Repair Mechanisms
Kyorin University
2019-2025
UNSW Sydney
2023
Yokohama City University
2022
Yokohama City University Medical Center
2022
Chuo University
2013-2022
University of Tsukuba
2004-2021
Yamagata University
2009-2021
Kitasato University
2020-2021
Yokohama Municipal Citizen's Hospital
2021
Yokohama City Seibu Hospital
2018
Daily synchronous rhythms of cell division at the tissue or organism level are observed in many species and suggest that circadian clock cycle oscillators coupled. For mammals, despite known mechanistic interactions, effect such coupling on progression, hence its biological relevance, is not understood. In particular, we do know how temporal organization single-cell produces this daily rhythm level. Here use multispectral imaging single live cells, computational methods, mathematical...
We identified a dominant missense mutation in the SCN transcription factor Zfhx3, termed short circuit (Zfhx3(Sci)), which accelerates circadian locomotor rhythms mice. ZFHX3 regulates via direct interaction with predicted AT motifs target genes. The mutant protein has decreased ability to activate consensus vitro. Using RNA sequencing, we found minimal effects on core clock genes Zfhx3(Sci/+) SCN, whereas expression of neuropeptides critical for intercellular signaling was significantly...
Novel dual thermoresponsive block copolymers displaying lower critical solution temperature (LCST) and upper (UCST) were synthesized by reversible addition−fragmentation chain transfer (RAFT) polymerization of two proline-based monomers. Poly(N-acryloyl-l-proline methyl ester), poly(A-Pro-OMe), was selected as a segment, whereas poly(N-acryloyl-4-trans-hydroxy-l-proline), poly(A-Hyp-OH), could be regarded water-soluble polymer. The copolymer having suitable comonomer composition...
To investigate alterations in circulating microRNAs (miRNAs) the development of experimental autoimmune uveoretinitis (EAU) rats. Lewis rats were immunized with interphotoreceptor retinoid binding protein (IRBP) peptide (R14) and EAU clinical scores assessed on day 0 (baseline), days 7, 14, 21 after immunization. Total RNA was isolated from serum at same timepoints used for microarray analysis. The score peaked 14 decreased 21. Hierarchical cluster analysis principal component (PCA) miRNA...
Abstract The SET‐CAN fusion gene is the product of a chromosomal rearrangement found on 9q34 associated with an acute undifferentiated leukemia. encodes almost complete SET protein fused to C‐terminal two‐thirds CAN. also known as TAF‐Iβ, histone chaperone and intracellular inhibitor phosphatase 2A, whereas CAN identical Nup214, nucleoporin protein. To obtain insight into leukemogenic function SET/TAF‐Iβ‐CAN/Nup214, we have examined its subcellular localization. Immunofluorescence analyses...
Nuclear-cytoplasmic transport through nuclear pore complexes is mediated by receptors. Previous reports have suggested that aberrant nuclear-cytoplasmic due to mutations or overexpression of and receptors closely linked diseases. Nup214, a component complexes, has been found as chimeric fusion proteins in leukemia. Among various Nup214 proteins, SET-Nup214 DEK-Nup214 shown be engaged tumorigenesis, but their oncogenic mechanisms remain unclear. In this study, we examined the functions...
Mutagenesis studies on glucose oxidases (GOxs) were conducted to construct GOxs with reduced oxidase activity and increased dehydrogenase activity. We focused two representative GOxs, of which crystal structures have already been reported—Penicillium amagasakiense GOx (PDB ID; 1gpe) Aspergillus niger 1cf3). constructed oxygen-interacting structural models for predicted the residues responsible oxidative half reaction oxygen basis structure cholesterol as well fact that both enzymes are...
Human myxovirus resistance gene A (MxA) is a type I interferon-inducible protein and exhibits the antiviral activity against variety of RNA viruses, including influenza virus. Previously, we reported that MxA accelerates cell death virus-infected cells through caspase-dependent -independent mechanisms. Similar to other virus infection induces endoplasmic reticulum (ER) stress, which one inducers. Here, have demonstrated enhances ER stress signaling in infected with stress-induced events,...
Despite the sequence and structural conservation between cryptochromes photolyases, members of cryptochrome/photolyase (flavo)protein family, their functions are divergent. Whereas photolyases DNA repair enzymes that use visible light to lesion-specifically remove UV-induced damage, act as photoreceptors circadian clock proteins. To address functional diversity we investigated effect ectopically expressed Arabidopsis thaliana (6-4)PP photolyase Potorous tridactylus CPD-photolyase (close...
We have successfully prepared polymeric micelles based on polyvinyl caprolactam–polyvinyl acetate–polyethylene glycol graft copolymer (Soluplus®) for a drug delivery system biofilm.
Abstract SET‐CAN associated with the t(9;9) in acute undifferentiated leukemia encodes almost entire sequence of SET and C‐terminal two‐third portion CAN, including FG repeat region. To clarify a role(s) leukemogenesis, we developed transgenic mice expressing under control Gata1 gene hematopoietic regulatory domain that is active distinct sets cells. showed anemia, thrombocytopenia, splenomegaly. A significant number started dying after 6 months post‐birth, being good agreement fact red...
By gating cell cycle progression to specific times of the day, intracellular circadian clock is thought reduce exposure replicating cells potentially hazardous environmental and endogenous genotoxic compounds. Although core gene defects that eradicate rhythmicity can cause an altered in vivo stress response aberrant proliferation rate, it remains be determined what extent these related phenotypes are due a cell-autonomous lack oscillations. We investigated DNA damage sensitivity...
To compare the effects of macular intraretinal hemorrhage (IRH) and hole (MH) on best-corrected visual acuity (BCVA) after displacement submacular (SMH) due to retinal arterial macroaneurysm (RAM) rupture.This multicenter retrospective study included 48 eyes with SMH RAM rupture. Cases underwent vitrectomy displace were followed up for 6 months. We classified cases according presence IRH MH compared postoperative BCVA among groups.We into IRH(+)MH(+) group (10 eyes), IRH(+)MH(-) (23...
Nucleolin (NCL) is a nucleolar protein i.e. involved in the regulation of structure and functions, consists three distinct regions: N-terminal region; middle region, which contains four RNA-recognition motifs (RRMs); C-terminal glycine- arginine-rich (GAR) region. The primary function RRMs GAR thought to be specific RNA binding. However, it not well understood how these RNA-binding regions NCL separately or cooperatively regulate its localization functions. To address this issue, we...
Thoracoscopic anatomical single or combined individual basilar segmentectomy, including subsegmentectomy, is technically challenging due to variations and the deep location of vessels bronchi in parenchyma. However, long-term perioperative outcomes various subsegmentectomy approaches have not been reported. Thus, we investigated effectiveness thoracoscopic segmentectomy.We evaluated records 119 patients who underwent complex segmentectomy between January 2005 December 2020 compared fissure...