A5064822303- Parasites and Host Interactions
- IL-33, ST2, and ILC Pathways
- Immune responses and vaccinations
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Mosquito-borne diseases and control
- T-cell and B-cell Immunology
- Zoonotic diseases and public health
- Research on Leishmaniasis Studies
- Gene expression and cancer classification
- Dietary Effects on Health
- Dermatology and Skin Diseases
- Parasite Biology and Host Interactions
- Diverse Scientific Research Studies
- Virology and Viral Diseases
- Immune cells in cancer
- COVID-19 Impact on Reproduction
- Metabolomics and Mass Spectrometry Studies
- Monoclonal and Polyclonal Antibodies Research
- Tuberculosis Research and Epidemiology
- Vitamin K Research Studies
- HIV Research and Treatment
- Galectins and Cancer Biology
Leiden University Medical Center
2018-2024
Rockefeller University
2023
Leiden University
2020
Mass cytometry identifies clusters of type 2 and regulatory immune responses in pre– post–anthelmintic treatment human blood samples.
Schistosomiasis is an infection caused by contact with
Multi-parametric flow and mass cytometry allows exceptional high-resolution exploration of the cellular composition immune system. A large panel computational tools have been developed to analyze high-dimensional landscape data generated. Analysis frameworks such as FlowSOM or Cytosplore incorporate clustering dimensionality reduction techniques include algorithms allowing visualization multi-parametric cytometric analysis. To additionally provide means quantify specific cell clusters...
Schistosomiasis affects over 250 million people, predominantly in Sub-Saharan Africa. In Schistosoma-endemic regions a lack of natural sterilizing immunity means individual are repeatedly infected, treated and reinfected. Due to difficulties tracking infection endemic areas, the kinetics host immune response during these reinfections have not been elucidated. Here, we use repeated (3x) controlled human Schistosoma mansoni (Sm) (repeated CHI) directly study how antigen-specific T cells...
Innate mononuclear phagocytic system (MPS) cells preserve mucosal immune homeostasis. We investigated their role at nasal mucosa following allergen challenge with house dust mite. combined single-cell proteome and transcriptome profiling on from biopsies 30 allergic rhinitis 27 non-allergic subjects before after repeated challenge. Biopsies of patients showed infiltrating inflammatory HLA-DRhi/CD14+ CD16+ monocytes proallergic transcriptional changes in resident CD1C+/CD1A+ conventional...
Vaccination of malaria-naive volunteers with a high dose Plasmodium falciparum sporozoites chemoattenuated by chloroquine (CQ) (PfSPZ-CVac [CQ]) has previously demonstrated full protection against controlled human malaria infection (CHMI). However, lower doses PfSPZ-CVac [CQ] resulted in incomplete protection. This provides the opportunity to understand immune mechanisms needed for better vaccine-induced comparing individuals who were protected those not protected. Using mass cytometry, we...
B-cells play a critical role in the formation of immune responses against pathogens by acting as antigen-presenting cells, modulating and generating memory antibody responses. Here, we studied B-cell subset distributions between regions with higher lower microbial exposure, i.e. comparing peripheral blood from people living Indonesia or Ghana to those healthy Dutch residents using 36-marker mass cytometry panel. By applying an unbiased multidimensional approach, observed differences balance...
Two hookworm vaccine candidates, Na-GST-1 and Na-APR-1, formulated with Glucopyranosyl Lipid A (GLA-AF) adjuvant, have been shown to be safe, well tolerated, induce antibody responses in a Phase 1 clinical trial (Clinicaltrials.gov NCT02126462) conducted Gabon. Here, we characterized T cell 24 Gabonese volunteers randomized get vaccinated three times Na-APR-1 at doses of 30μg (n = 8) or 100μg 10) as control Hepatitis B 6). Blood was collected pre- post-vaccination on days 0, 28, 180 2-weeks...
The complexity of data generated by mass cytometry has necessitated new tools to rapidly visualize analytic outcomes. Clustering methods like Cytosplore or FlowSOM are used for the visualization and identification cell clusters. For downstream analysis, a newly developed R package, Cytofast, can generate rapid results from clustering methods. Cytofast takes into account phenotypic characterization clusters, calculates cluster abundance, then quantitatively compares groups. This protocol...
Antibody glycosylation patterns can affect antibody functionality and thereby contribute to protection against invading pathogens. During pregnancy, maternal antibodies be transferred through the placenta modulating both mother's her child's immune responses. Although several studies of IgG during pregnancy have been carried out, very few cohorts studied were from sub-Saharan Africa, where exposure microorganisms parasites is high. In Lambaréné, Gabon, 106 pregnant women in their third...
The complexity of data generated by mass cytometry has necessitated new tools to rapidly visualize analytic outcomes. Clustering methods like Cytosplore or FlowSOM are used for the visualization and identification cell clusters. For downstream analysis, a newly developed R package, Cytofast, can generate rapid results from clustering methods. Cytofast takes into account phenotypic characterization clusters, calculates cluster abundance, then quantitatively compares groups. This protocol...
Early expansion of allergen-responsive LAP+ B regulatory cells in allergic rhinitis but not asthma subjects during allergen immunotherapyAstrid L. Voskamp1, Nicolette W. de Jong2, Simon P. Jochems1, Arifa Ozir-Fazalalikhan1, Luciën E.P.M. van der Vlugt1, Koen A. Stam1, Gert-Jan Braunstahl3,4, Gertrude M. Möller5, Roy Gerth Wijk2, Hermelijn H. Smits11Department Parasitology, Leiden University Center for Infectious Diseases (LU-CID), Medical Center; Leiden, The Netherlands.2Dept Internal...
Abstract Schistosomiasis is a prevalent helminthiasis, affecting over 230 million people worldwide, with varied, stage specific morbidity. Whilst the Th2 and regulatory immune responses in chronic infection have been relatively well studied, we little understanding of human during acute infection. This despite initial infective stages being proposed as crucial targets for much-needed vaccine development. Here, comprehensively map male female single-sex controlled Schistosoma mansoni Using...
Abstract Innate mononuclear phagocytic system (MPS) cells preserve mucosal immune homeostasis. Here, we investigated their role at nasal mucosa following challenge with house dust mite. We combined single cell proteome and transcriptome profiling on from biopsy of allergic rhinitis non-allergic subjects, before after repeated allergen challenge. Nasal biopsies patients showed infiltrating inflammatory HLA-DR hi CD14 + monocytes CD16 monocytes, transcriptional changes in resident CD1C CD1A...
Abstract BACKGROUND Vaccinations against infectious diseases save millions of lives, however reduced vaccine responses have been observed in low-income settings. Therefore, understanding differences immune profiles populations with high or low socioeconomic status (SES) is importance for optimizing efficacy. AIM Examining the profile children versus SES city Makassar, Indonesia. METHODS Using mass cytometry, we profiled cells finger-prick blood samples attending and schools RESULTS Distinct...
Metabolomics provides a powerful tool to study physiological changes in response various perturbations such as vaccination. We explored whether metabolomic could be seen after vaccination phase I trial where Gabonese adults living either rural or semi-urban areas received the subunit hookworm vaccine candidates ( Na -GST-1 and -APR-1 (M74) adjuvanted with Alhydrogel plus GLA-AF (n = 24) hepatitis B 8) control. Urine samples were collected assayed using targeted 1 H NMR spectroscopy. At...