A5070201537- Bioinformatics and Genomic Networks
- Neurobiology and Insect Physiology Research
- Gene expression and cancer classification
- Gene Regulatory Network Analysis
- Genomics and Chromatin Dynamics
- Glycosylation and Glycoproteins Research
- Developmental Biology and Gene Regulation
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Autism Spectrum Disorder Research
- Plant nutrient uptake and metabolism
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolic Engineering and Bioproduction
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Legume Nitrogen Fixing Symbiosis
- Cancer Genomics and Diagnostics
- Insect-Plant Interactions and Control
- Molecular Biology Techniques and Applications
- Animal Genetics and Reproduction
- Genetics and Neurodevelopmental Disorders
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Rare Diseases
- Plant Reproductive Biology
- Diphtheria, Corynebacterium, and Tetanus
Tampere University
2013-2015
Tampere University of Applied Sciences
2013
MSD (Finland)
2010
University of Helsinki
1991-2004
Medicel (Switzerland)
2004
Biocenter Finland
2001-2002
Massachusetts General Hospital
2001
Helsinki University Hospital
2001
Harvard University
2001
Helsinki Institute of Physics
1995-1997
This study aimed at establishing bacterial flagellin-recognizing toll-like receptor 5 (TLR5) as a novel link between gut microbiota composition, adipose tissue inflammation, and obesity. An microarray database was used to compare women having the highest (n = 4, H-TLR) lowest L-TLR) expression levels of TLR5-signaling pathway genes. Gut composition profiled using flow cytometry FISH. Standard laboratory techniques were determine anthropometric clinical variables. In vivo results verified...
The whole genome approach enables the characterization of all components any given biological pathway. Moreover, it can help to uncover metabolic routes for molecule. Here we have used <i>Drosophila melanogaster</i> search enzymes involved in metabolism fucosylated glycans. Our results suggest that fruit fly GDP-fucose, donor fucosyltransferase reactions, is formed exclusively via <i>de novo</i> pathway from GDP-mannose through enzymatic reactions catalyzed by GDP-d-mannose 4,6-dehydratase...
Rhizobia are soil bacteria able to fix atmospheric nitrogen in symbiosis with leguminous plants. In response a signal cascade coded by genes of both symbiotic partners, specific plant organ, the nodule, is formed. Rhizobial nodulation (nod) trigger nodule formation through synthesis Nod factors, family chitolipooligosaccharides that specifically recognized host at first stages process. Here, we present organization and sequence common nod from Rhizobium galegae, member RHIZOBIACEAE: This...
Autism Spectrum Disorder (ASD) affects approximately 1 child in 54, with a 35-fold increase since 1960. Selected studies suggest that part of the recent prevalence is likely attributable to an improved awareness and recognition, changes clinical practice or service availability. However, this not sufficient explain epidemiological phenomenon. Research points possible link between ASD intestinal microbiota because many children display gastro-intestinal problems. Current large-scale datasets...
In Drosophila, the establishment of dorsoventral polarity developing embryo depends on expression at least 11 maternally acting genes. Mutant females that lack any these gene activities produce normally shaped eggs develop into dorsalized embryos. The female sterile K10 mutation differs from mutants, because in addition to development embryo, it causes a dorsalization egg shape. During oogenesis, is specifically expressed oocyte. Antibodies raised against beta-galactosidase-K10 fusion...
The diazepam-binding inhibitor (DBI; also called acyl coenzyme A-binding protein or endozepine) is a 10-kDa polypeptide found in organisms ranging from yeasts to mammals. It has been shown that DBI and its processing products are involved various specific biological processes such as GABAA/benzodiazepine receptor modulation, A metabolism, steroidogenesis, insulin secretion. We have cloned sequenced the Drosophila melanogaster gene cDNA encoding DBI. encodes of 86 amino acids shows 51 56%...
Abstract Next-generation sequencing (NGS) is replacing other molecular techniques to become the de facto gene diagnostics approach, transforming speed of diagnosis for patients and expanding opportunities precision medicine. Consequently, accredited laboratories as well those seeking accreditation, both objective measures quality external review laboratory processes are required. External assessment (EQA), or Proficiency Testing (PT), can assess a laboratory’s service through an independent...
Summary and Discussion B. influenzae can be found in every case of true clinical influenza. To isolate this organism, which is most abundant the earlier stages disease, it necessary to exercise care obtaining a suitable specimen, since growth requirements organism are quite rigid, special selective culture media, such as those suggested by Avery (24) also Fleming (25), carefully prepared adjusted reaction essential for successful work. The various strains apparently do not differ kind. This...
The diazepam-binding inhibitor (DBI; also called acyl coenzyme A-binding protein or endozepine) is a 10-kDa polypeptide found in organisms ranging from yeasts to mammals. It has been shown that DBI and its processing products are involved various specific biological processes such as GABAA/benzodiazepine receptor modulation, A metabolism, steroidogenesis, insulin secretion. We have cloned sequenced the Drosophila melanogaster gene cDNA encoding DBI. encodes of 86 amino acids shows 51 56%...
Pseudomonas aeruginosa is an opportunistic Gram‐negative bacterium that causes severe infections in a number of hosts from plants to mammals. A‐band lipopolysaccharide P. contains d ‐rhamnosylated O‐antigen. The synthesis GDP‐ ‐rhamnose, the ‐rhamnose donor ‐rhamnosylation, starts ‐mannose. It first converted by GDP‐mannose‐4,6‐dehydratase (GMD) into GDP‐4‐keto‐6‐deoxy‐ ‐mannose, and then reduced ‐mannose reductase (RMD). Here, we describe enzymatic characterization RMD expressed...
High-throughput genomic measurements, interpreted as cooccurring data samples from multiple sources, open up a fresh problem for machine learning: What is in common the different sets, that is, what kind of statistical dependencies are there between paired sets? We introduce clustering algorithm exploring dependencies. Samples within each set grouped such groups sets capture much pairwise possible. formalize this novel probabilistic way, optimization Bayes factor. The method applied to...
Helicobacter pylori is a Gram‐negative gastric pathogen causing diseases from mild infections to cancer. The difference in clinical outcome has been suggested be due strain differences. H. undergoes phase variation by changing its lipopolysaccharide structure according the environmental conditions. O‐antigen of contains fucosylated glycans, similar Lewis structures found human epithelium. These glycans have play role pathogenesis adhesion bacterium In synthesis structures, GDP‐ l ‐fucose...
In the salvage pathway of GDP‐ l ‐fucose, free cytosolic fucose is phosphorylated by ‐fucokinase to form ‐fucose‐1‐phosphate, which then further converted ‐fucose in reaction catalyzed pyrophosphorylase. We report here cloning and expression murine Murine expressed as two transcripts 3057 3270 base pairs, encoding proteins 1019 1090 amino acids with predicted molecular masses 111 kDa 120 respectively. Only longer splice variant was enzymatically active when COS‐7 cells. pyrophosphorylase has...
Molecular interaction networks establish all cell biological processes. The are under intensive research that is facilitated by new high-throughput measurement techniques for the detection, quantification, and characterization of molecules their physical interactions. For common model organism yeast Saccharomyces cerevisiae, public databases store a significant part accumulated information and, on way to better understanding cellular processes, there need integrate this into consistent...
Rhizobial nodD genes produce transcriptional regulators that, together with appropriate inducer compounds, activate the other symbiotic nodulation (nod) and initiate nodule formation process. Two homologues, 1 2, are present in Rhizobium galegae strain HAMBI 1174. In this work we analysed their ability to induce nodA promoter synthetic inducers known nod rhizobia. According phylogenetic analysis, inducer-specific carboxy-terminal part of R. protein sequence groups those leguminosarum...
Halanaerobium saccharolyticum is a halophilic anaerobic fermentative bacterium capable of producing hydrogen, potential future energy carrier molecule. The high-quality draft genome H. subsp. strain DSM 6643(T) consists 24 contigs for 2,873,865 bp with G+C content 32.3%.
Abstract Background Autism spectrum disorder (ASD) is a partially heritable neurodevelopmental trait, and people with ASD may also have other co-occurring trait such as ADHD, anxiety disorders, depression, mental health issues, learning difficulty, physical traits communication challenges. The concomitant development of neurological assumed to result from complex interplay between genetics the environment. However, only limited number studies performed multivariate genome-wide association...
Caloramator celer strain JW/YL-NZ35 is a Gram-positive thermophilic, alkalitolerant, and strictly anaerobic bacterium capable of producing hydrogen ethanol under extreme conditions. The draft genome sequence presented here will provide valuable information to further explore the physiology this species its potential for biofuel production.