A5102889398- Autophagy in Disease and Therapy
- Neuroinflammation and Neurodegeneration Mechanisms
- S100 Proteins and Annexins
- Menopause: Health Impacts and Treatments
- Estrogen and related hormone effects
- MicroRNA in disease regulation
- Parkinson's Disease Mechanisms and Treatments
- Cancer Cells and Metastasis
- Mechanisms of cancer metastasis
- Cancer-related molecular mechanisms research
- Histone Deacetylase Inhibitors Research
- Cancer Mechanisms and Therapy
- Inflammatory mediators and NSAID effects
- Sirtuins and Resveratrol in Medicine
- Cancer-related gene regulation
- Proteoglycans and glycosaminoglycans research
- Anesthesia and Neurotoxicity Research
- 3D Printing in Biomedical Research
- interferon and immune responses
- Genetic and phenotypic traits in livestock
- Galectins and Cancer Biology
- Biochemical effects in animals
- Trace Elements in Health
- Saffron Plant Research Studies
- Receptor Mechanisms and Signaling
Tianjin Medical University Cancer Institute and Hospital
2022-2025
Soochow University
2018-2024
Xiangya Hospital Central South University
2017-2022
Central South University
2017-2022
Second Affiliated Hospital of Soochow University
2018-2022
First Affiliated Hospital of Xi'an Jiaotong University
2021
Air Force Medical University
2017-2019
Xijing Hospital
2017-2019
Hebei Agricultural University
2007
Wan Fang Hospital
2004
The steroid hormone estrogen (17-β-estradiol, E2) provides neuroprotection against cerebral ischemic injury by activating receptors. novel receptor G protein-coupled 30 (GPR30) is highly expressed in the brain and acute stroke. However, underlying mechanisms remain unclear.In this study, ovariectomized female mice were subjected to middle artery occlusion (MCAO), E2, G1, ICI182780 administered immediately upon reperfusion. infarction volume, neurological scores, neuronal injuries examined....
Astrocytes possess different morphological characteristics depending on the cerebral region in which they are found. However, none of current astrocytic markers can label all subpopulations successfully. Thus, identifying appropriate marker for a specific scientific investigation is critical. Here, we compared distribution and protein expression three astrocyte markers: NDRG2, GFAP, S100 β , cortex, hippocampus, thalamus. NDRG2- -positive astrocytes were distributed more uniformly than...
The prognosis of advanced non-small cell lung cancer (NSCLC) patients is poor. One the reasons for this hampered progress has been a lack in vitro models that would faithfully recapitulate heterogeneity tumors and response to treatment. In study, surgically resected were obtained from with stage I/II NSCLC during curative-intent surgery. Using 3D patient-derived tumor spheroids culture system, our results demonstrate successful long-term expansion primary cells (> 120 days). Patient-derived...
Microglial inflammation is a hallmark of ischemic stroke. Annexin A3 (ANXA3) expressed in microglia and plays detrimental role However, the ANXA3 microglial after stroke unclear. In this study, an model was established mice via middle cerebral artery occlusion (MCAO). The adeno-associated virus shANXA3 (AAV-shANXA3) injected into ipsilateral cortex lesion, infarction volume, neurological score, neuronal injury were examined. Moreover, primary transfected with lentivirus (LV-shANXA3)...
Abstract Bone cancer-induced pain (BCP) is a challenging clinical problem because traditional therapies are often only partially effective. Annexin A3 (ANXA3) highly expressed in microglia the spinal cord, and its expression upregulated during BCP. However, roles of microglial ANXA3 development maintenance BCP underlying molecular mechanisms remain unclear. This study was performed on male mice using metastatic lung model. Adeno-associated virus shANXA3 (AAV-shANXA3) injected intrathecally...
Inflammation is a key element in the pathophysiology of cerebral ischemia. This study investigated role N-Myc downstream-regulated gene-2 nuclear transcription factor κB-mediated inflammation ischemia models.Mice (n = 6 to 12) with or without κB inhibitor pyrrolidinedithiocarbamate pretreatment were subjected global for 20 min. Pure astrocyte cultures astrocyte-neuron cocultures 6) exposed oxygen-glucose deprivation 4 h 2 h. Astrocytic and expression, proinflammatory cytokine secretion,...
Long-term morphine administration leads to tolerance and a gradual reduction in analgesic potency. Noncoding microRNAs (miRNAs) modulate gene expression posttranscriptional manner, their dysregulation causes various diseases. Emerging evidence suggests that miRNAs play regulatory role the development of tolerance. In present study, we hypothesized miR-873a-5p is key functional small RNA participates maintenance through regulation A20 (tumor necrosis factor α-induced protein 3, TNFAIP3) mice....
The parenchymal microglia possess different morphological characteristics in cerebral physiological and pathological conditions; thus, visualizing these cells is useful as a means of further investigating microglial function. Annexin A3 (ANXA3) expressed microglia, but it unknown whether can be used marker protein for its Here, we compared the distribution morphology labeled by ANXA3, cluster differentiation 11b (CD11b), ionized calcium-binding adaptor molecule 1 (Iba1) measured expression...
Abstract Astrocyte undergoes morphology changes that are closely associated with the signaling communications at synapses. N‐myc downstream‐regulated gene 2 (NDRG2) is specifically expressed in astrocytes and several important astrocyte functions, but its potential role(s) relating to morphological remain unknown. Here, primary were prepared from neonatal Ndrg2 +/+ −/− pups, drug Y27632 was used induce stellation. We then a variety of methods measure levels NDRG2, α‐Actinin4, glial...
Abstract Neurons rely heavily on high mitochondrial metabolism to provide sufficient energy for proper development. However, it remains unclear how neurons maintain oxidative phosphorylation (OXPHOS) during Mitophagy plays a pivotal role in maintaining quality and quantity. We herein describe that G protein-coupled receptor 50 (GPR50) is novel mitophagy receptor, which harbors the LC3-interacting region (LIR) required under stress conditions. Although does not localize mitochondria normal...
Human endophilin 1 (hEndo1) is a multifunctional protein that was found to bind wide spectrum of prolinerich endocytic proteins through its Src homology 3 (SH3) domain. In order elucidate the unknown biological functions hEndo1, it essential find out cytoplasmic components hEndo1 recognizes and binds. However, too time-consuming expensive synthesize all peptide candidates in human proteome perform SH3–peptide affinity assay identify hEndo1-binding partners. present work, we describe...
Gefitinib, a well-known epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor for the targeted therapy of lung cancer, induces autophagy in association with drug resistance. However, it remains unclear whether gefitinib treatment can affect selective form (i.e., mitophagy) and be beneficial human diseases decreased autophagy, such as neurodegenerative diseases. Here, we show that promotes PINK1/Parkin-mediated mitophagy both nonneuronal neuronal cells, this effect is independent...
Lung cancer is the leading cause of cancer-related death worldwide. Overall survival all stages unsatisfactory due to high metastasis and recurrence rates. Recent studies indicated that epithelial-to-mesenchymal transition (EMT) involved in progression cancer. Some reports also indicate HS3ST3B1 angiogenesis proliferation cells. However, its role non-small cell lung (NSCLC) not well known. In this study, we found was significantly up-regulated NSCLC tissues compared with matched normal...