A5100627155- Pharmacological Effects of Natural Compounds
- Multiple Myeloma Research and Treatments
- Cancer, Lipids, and Metabolism
- Cancer Cells and Metastasis
- Cholesterol and Lipid Metabolism
- Chromatography in Natural Products
- Bioactive Compounds and Antitumor Agents
- Cancer-related Molecular Pathways
- Natural product bioactivities and synthesis
- Wnt/β-catenin signaling in development and cancer
- Cancer, Hypoxia, and Metabolism
- Drug Transport and Resistance Mechanisms
- Hormonal Regulation and Hypertension
- Protein Degradation and Inhibitors
- Cell Adhesion Molecules Research
- Epigenetics and DNA Methylation
- Ginseng Biological Effects and Applications
- Cancer Research and Treatments
- NF-κB Signaling Pathways
- Lipid metabolism and biosynthesis
- Cancer survivorship and care
- Radiomics and Machine Learning in Medical Imaging
- Chronic Myeloid Leukemia Treatments
- CAR-T cell therapy research
- Inflammatory Bowel Disease
Johnson & Johnson (United States)
2025
Hebei University of Chinese Medicine
2017-2024
Chongqing University of Science and Technology
2024
Hospital of Hebei Province
2022-2024
Janssen (United States)
2024
Fudan University
2014-2023
Zhejiang Medicine (China)
2022
Changhong (China)
2022
Army Medical University
2009-2019
The Affiliated Yongchuan Hospital of Chongqing Medical University
2019
Hypoxia in tumors is generally associated with chemoresistance and radioresistance. However, the correlation between heterodimeric hypoxia-inducible factor-1 (HIF-1) multidrug resistance (MDR1) gene/transporter P-glycoprotein (P-gp) has not been clearly investigated. This study aims at examining expression levels of HIF-1α MDR1/P-gp human colon carcinoma tissues cell lines (HCT-116, HT-29, LoVo, SW480) ascertaining whether plays an important role tumor MDR1/P-gp. The distribution P-gp...
Because colorectal cancer (CRC) stem-like cells (CCS-like cells) contribute to poor patient prognosis, these are a potential target for CRC therapy. However, the mechanism underlying maintenance of CCS-like cell properties remains unclear. Here, we found that patients with advanced stage expressed high levels polycomb group protein enhancer zeste homologue 2 (EZH2). High expression EZH2 in tumor tissues correlated prognosis. Conversely, silencing reduced proliferation. Surprisingly, was more...
Purpose To investigate the correlation between chemoresistance of colorectal cancer to 5-fluorouracil and BNIP3 underlying mechanism. Methods protein in specimens was evaluated using immunohistochemistry. Semi-quantitative reverse transcription PCR Western blot employed assay gene expression. The promoter methylation status examined by methylation-specific PCR. Drug sensitivity assayed MTT assay. Results Specimens from 81 patients with receiving 5-fluorouracil-based chemotherapy were...
This study seeks to discover flavonoids from a traditional Chinese herb, Artemisia rupestris L., with synergistic antibacterial effects against multidrug-resistant Staphylococcus aureus. Five flavonoids, artemetin (1), chrysosplenetin (2), pachypodol (3), penduletin (4) and chrysoeriol (5) were obtained by various column chromatographic methods. Their chemical structures determined on the basis of comprehensive spectroscopic analysis comparison literature data. Three compounds (2, 4 5)...
miR-186-5p has been reported to be implicated in tumorigenesis and paclitaxel resistance non-small-cell lung cancer cells (NSCLCs). However, it remains undisclosed whether takes a part chemoresistance against diaminodichloroplatinum (cisplatin, DDP) cancers, including NSCLC. Expression of sine oculis homeobox 1 (SIX1) was detected using RT-qPCR western blot. In vitro, 50% inhibitory concentration (IC50) DDP cell proliferation were measured by MTT assay. The rate apoptosis abilities migration...
Colorectal cancer is strongly associated with lipid metabolism. NPC1L1, a sterol transporter, plays key role in modulating homeostasis vivo. Its inhibitor, ezetimibe, began to be used clinically lower cholesterol and this caused the great debate on its causing carcinogenesis. Here we explored of NPC1L1 colorectal tumorigenesis.Wild-type mice NPC1L1(-/-) (NPC1L1 knockout) were treated azoxymethane (AOM)-dextran sodium sulfate (DSS) induce colitis-associated tumorigenesis. Mice sacrificed 10,...
// Jianming He 1, 2, * , Jinming Zhou Weiwen Yang 1 Qi Xi Liang 3 Xueli Pang Jianjun Li Feng Pan and Houjie Department of Oncology Southwest Cancer Center, Hospital, Third Military Medical University, Chongqing 400038, China 2 Radiotherapy, Hebei Provincial Hospital Traditional Chinese Medicine, University Shijiazhuang 050011, Radiology, These authors have contributed equally to this work Correspondence to: Liang, email: lianghoujie@sina.com Keywords: glucocorticoid, glucocorticoid receptor,...
To investigate the roles of high-mobility group box 1 (HMGB1) protein in lymphangiogenesis and lymphatic node metastasis colon cancer.Archival tumour specimens from patients with cancer were analysed this retrospective immunohistochemical study. HMGB1, vascular endothelial growth factor C (VEGF-C) podoplanin levels immunohistochemically. In vitro studies using cell line HCT116 also undertaken to relationship between VEGF-C nuclear (NF)-κB.Specimens 70 reviewed. The presence positive HMGB1...
VGLL4, a member of the Vestigial-like (VGLL) proteins, has been reported to be dysregulated in several cancer types. However, its function esophageal squamous cell carcinoma (ESCC) remains poorly understood. Here, it was found that expression level VGLL4 decreased ESCC tissues. Moreover, forced cells inhibited growth and migration, while knockdown promoted tumorigenecity cells. Mechanistically, regulated motility through downregulating connective tissue factor (CTGF), known oncogene...
Psychological stress is associated with an increased risk of cardiovascular diseases. However, the connecting mechanisms stress-inducing activation hypothalamic-pituitary-adrenal axis atherosclerosis are not well-understood.To study effect acute psychological on reverse cholesterol transport (RCT), which transfers peripheral to liver for its ultimate fecal excretion.C57Bl/6J mice were exposed restraint 3 hours induce stress. RCT in vivo was quantified by measuring transfer [(3)H]cholesterol...
Dysregulated long non-coding RNAs (lncRNAs) might exert critical roles in pathways associated with gastric cancer (GC) development. Long RNA (SNHG17), a newly identified lncRNA, has been reported to be dysregulated several tumors. Our present study aimed explore the expression level of SNHG17 patients GC and investigate relationship between prognosis patients.The levels were detected by Real-time-quantitative Polymerase Chain Reaction (RT-qPCR). The chi-square test was performed associations...
Panax ginseng has been used in traditional oriental medicine for thousands of years. Ginsenosides, the major chemical components roots, are considered to be responsible medicinal properties ginseng. Ginsenosides increase with age root general knowledge, and this study content ginsenosides different ages was quantified. Separation determination eight main ginsenosides, Rgl, Re, Rbl, Rg2, Rb2, Rb3 Rd, performed by high performance liquid chromatography UV detection at 203 nm. The Rg2 Rd...
<h3>Background</h3> Sporadic inclusion body myositis (IBM) is the commonest idiopathic inflammatory myopathy occurring in patients over age of 50 years. IBM muscle displays and degenerative features. Previous trials have only involved agents directed purely at component pathology all were ineffective. Modulating cytoprotective "heat shock response" (HSR) represents a therapeutic strategy through which detrimental aspects both inflammation degeneration could be dampened. Arimoclomol an orally...
When cancer cells have been cultured as three-dimensional (3D) cultures or in vivo, they decrease sensitivity to anticancer drugs. This is called multicellular resistance, and the mechanism not fully understood. Here, we report that E-cadherin increasing multidrug resistance protein 1 (MDR1) via hypoxia-inducible factor-1α (HIF-1α) contributes colorectal cancer. The MDR1 level was higher 3D than monolayer cells. dispersed from were grown again, decreased similar of maintained all through....
Chemotherapy resistance in solid tumors is broad and encompasses diverse unrelated drugs. Three-dimensional multicellular spheroids (MCSs) are a good model for studying vitro drug resistance. In the current study, we investigated role of focal adhesion kinase (FAK) 5-fluorouracil (5-FU) chemoresistance colon carcinoma MCS culture cells. The expression FAK was inhibited significantly by specific small hairpin RNA targeting FAK. suppression did not affect growth spheroid However, silencing...
Three-dimensional (3D) culture models represent a better approximation of solid tumor tissue architecture, especially cell adhesion, in vivo than two-dimensional (2D) cultures do.Here, we explored the role architecture chemosensitivity to platinum colon cancer.Under 3D condition, cancer cells formed multicellular spheroids, consisting layers cells.3D displayed significantly decreased sensitivity compared with 2D cultures.Platinum increased p53 dose-dependent and time-dependent manner.There...