A5061339755- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Bacterial Infections and Vaccines
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- Plant Virus Research Studies
- Gut microbiota and health
- Malaria Research and Control
- Global Maternal and Child Health
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- Viral Infections and Immunology Research
- Biosensors and Analytical Detection
- Streptococcal Infections and Treatments
- Neonatal Respiratory Health Research
- COVID-19 epidemiological studies
- Bacterial biofilms and quorum sensing
- Amoebic Infections and Treatments
University of Science and Technology
2020-2023
Korea University of Science and Technology
2022-2023
Bernhard Nocht Institute for Tropical Medicine
2015-2019
Jashore University of Science and Technology
2019
German Center for Infection Research
2016
International Centre for Diarrhoeal Disease Research
2009-2015
Dhaka Shishu Hospital
2009-2014
Child Health Research Foundation
2013-2014
Boston Children's Hospital
2008
University of Dhaka
2008
The SARS-CoV-2 coronavirus is responsible for the current COVID-19 pandemic, with an ongoing toll of over 5 million infections and 333 thousand deaths worldwide within first months. Insight into phylodynamics mutation variants this virus vital to understanding nature its spread in different climate conditions. incidence rate increasing at alarming pace subtropical South-East Asian nations high temperatures humidity. To understand spread, we analysed 444 genome sequences available on GISAID...
Abstract Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) pandemic has been considered with great importance on correct screening procedure. The detection efficiency of recent variants concern were observed by comparing 5 commercial RT-PCR kits and a SYBR-green method developed validated in our laboratory. RNA was extracted from nasopharyngeal samples suspected COVID-19 patients assay performed according to the instruction respective manufacturers. specificity sensitivity Maccura...
Vaccines offer the prospect of primary disease prevention pneumococcal in childhood. For introduction such vaccines developing countries, information about epidemiology is necessary.We evaluated antimicrobial susceptibility and serotype distribution invasive Streptococcus pneumoniae children aged <5 years a network 7 hospitals Bangladesh from May 2004 through 2007.Of 17,969 blood cultures 3765 cerebrospinal fluid cultures, 139 yielded S. isolates; 94 were meningitis cases, 13 pneumonia 32...
Background PCR-based serotyping of Streptococcus pneumoniae has been proposed as a simpler approach than conventional methods, but not applied to strains in Asia where serotypes are diverse and different from other part the world. Furthermore, PCR used determine serotype distribution culture-negative meningitis cases. Methodology Thirty six serotype-specific primers, 7 newly designed 29 previously published, were arranged multiplex sets, each new hierarchies for overall Bangladesh,...
Streptococcus pneumoniae infection is recognized as a global priority public health problem, and conjugate vaccines have been shown to prevent vaccine-type invasive pneumococcal disease (IPD) in children. However, better estimates of the burden reliable population-based data on serotype composition are needed for vaccine development implementation developing countries.We initiated surveillance rural Bangladesh community Mirzapur, covering population approximately 144,000. Village care...
Background The relevance of Cryptosporidium infections for the burden childhood diarrhoea in endemic settings has been shown recent years. This study describes subtypes among symptomatic and asymptomatic children rural Ghana to analyse subtype-specific demographic, geographical, seasonal clinical differences order inform appropriate control measures areas. Methodology/Principal Findings Stool samples were collected from 2232 below 14 years age presenting with without gastrointestinal...
In 2023, the dengue virus (DENV) outbreak infected over 0.3 million cases and 1500 deaths in Bangladesh. Our study conducted serotyping genomic surveillance four districts of Southwest Bangladesh between September October 2023. The data from 2019 to 2023 extracted Directorate General Health Services indicated a significant increase Dengue infections particularly during September–November. two‐layered hypothesis examination confirmed that, despite endemic months, had higher morbidity rate...
Abstract Lactic acid bacteria are the well acknowledged probiotics that can cure a variety of diseases. In this study, we observed in vivo potentials Pediococcus to treat hyperglycemia, hypercholesterolemia and gastrointestinal infections. A total 77 Lactobacillus were isolated from milk 10 cows goats, four those strains inhibited both carbohydrates-hydrolyzing enzymes, α-glucosidase, α-amylase. They all showed antagonistic effects on pathogenic E. coli S. Typhimurium which confirmed by...
Background. Salmonella ranks among the leading causes of bloodstream infections in sub-Saharan Africa. Multidrug resistant typhoidal and nontyphoidal (NTS) isolates have been previously identified this region. However, resistance to ciprofloxacin has rarely reported West This study aims assess susceptibility against causing invasive children rural Ghana. Methods. From May 2007 until 2012, attending a district hospital central Ghana were eligible for recruitment. enterica isolated from blood...
Methicillin-resistant Staphylococcus aureus (MRSA) clones pose a significant threat to hospitalised patients because the bacteria can be transmitted by asymptomatic carriers within healthcare facilities. To date, nothing is known about prevalence of S. and MRSA among workers in Madagascar. The objective our study was examine clonal epidemiology nasal non-medical University students Antananarivo, This cross sectional screened swabs taken from for aureus. Multiplex PCR performed identify...
Salmonella enterica serovar Typhi is a predominant cause of bloodstream infections in sub-Saharan Africa (SSA). Increasing numbers S. with resistance to ciprofloxacin have been reported from different parts the world. However, data SSA are limited. In this study, we aimed measure susceptibility isolated patients febrile illness SSA.Febrile 9 sites within 6 countries body temperature ≥38.0°C were enrolled study. Blood samples obtained for bacterial culture, and isolates identified...
Salmonella enterica serovar Enteritidis is a cause of both poultry- and egg-associated enterocolitis globally bloodstream-invasive nontyphoidal (iNTS) disease in sub-Saharan Africa (sSA). Distinct, multi-drug resistant genotypes associated with iNTS sSA have recently been described, often requiring treatment fluoroquinolone antibiotics. In industrialised countries, antimicrobial use poultry production has led to frequent resistance amongst prevalent enterocolitis-associated lineages.Twenty...
The epidemiology of pediatric febrile illness is shifting in sub-Saharan Africa, but malaria remains a major cause childhood morbidity and mortality. present study describes causes hospitalized children Ghana aims to determine the burden coinfections their association with parasite densities. In prospective study, (aged ≥30 days ≤15 years) fever ≥38.0°C were recruited after admission ward primary hospital Ghana. Malaria parasitemia was determined blood, stool, urine, respiratory,...
Abstract Tracing the globally circulating severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) phylogenetic clades by high‐throughput sequencing is costly, time‐consuming, and labor‐intensive. We here propose a rapid, simple, cost‐effective amplification refractory mutation system (ARMS)‐based multiplex reverse‐transcription polymerase chain reaction (PCR) assay to identify six distinct clades: S, L, V, G, GH, GR. Our PCR designed in mutually exclusive way V–S G–GH–GR clade variants...
The mortality of coronavirus disease 2019 (COVID-19) is very high among the elderly or individuals having comorbidities such as obesity, cardiovascular diseases, lung infections, hypertension, and/or diabetes. Our study characterizes metagenomic features in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-infected patients with without type 2 diabetes, to identify microbial interactions associated its fatal consequences.This compared baseline nasopharyngeal microbiome...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has evolved into eight fundamental clades with four of these (G, GH, GR, and GV) globally prevalent in 2020. To explain plausible epistatic effects the signature co‐occurring mutations circulating on viral replication transmission fitness, we proposed a hypothetical model using silico approach. Molecular docking dynamics analyses showed higher infectiousness spike mutant through more favorable binding G 614 elastase‐2....
Abstract TaqMan probe-based commercial real-time (RT) PCR kits are expensive but most frequently used in COVID-19 diagnosis. The unprecedented scale of SARS-CoV-2 infections needs to meet the challenge testing more persons at a reasonable cost. This study developed simple and cost-effective alternative diagnostic method based on melting curve analysis SYBR green multiplex assay targeting two virus-specific genes along with host-specific internal control. A total 180 randomly selected samples...
Background. The Typhoid Fever Surveillance in Africa Program (TSAP) estimated adjusted incidence rates (IRs) for Salmonella enterica serovar Typhi and invasive nontyphoidal S. serovars (iNTS) of >100 cases per 100 000 person-years observation (PYO) children aged <15 years Asante Akim North Municipal (AAN), Ghana, between March 2010 May 2012. We analyzed how much these differed rural urban settings. Methods. Children recruited at the Agogo Presbyterian Hospital meeting TSAP inclusion criteria...
Recent outbreaks of measles and polio in low-income countries illustrate that conventional methods for estimating vaccination coverage do not adequately identify susceptible children. Immune markers protection against vaccine-preventable diseases oral fluid (OF) or blood may generate more accurate measures effective history, but questions remain about whether antibody surveys are feasible informative tools monitoring immunization program performance compared to indicators. This study...
Detection of pneumococcal carriage by multiple co-colonizing serotypes is important in assessing the benefits conjugate vaccine (PCV). Various methods differing sensitivity, cost and technical complexity have been employed to detect pneumococcus respiratory specimens. We developed an algorithmic method all known that preserves relative abundance specific using Quellung-guided molecular techniques. The involves culturing swabs followed serotyping 100 colonies either capsular (10 colonies) or...
A multidrug-resistant Salmonella enterica serovar Typhimurium with reduced susceptibility to ciprofloxacin was isolated from the blood of a hospitalized child in Ghana. DNA sequencing identified novel gyrB mutation at codon 466 (Glu466Asp). An increase fluoroquinolone after introduction wild-type gyrB+ allele demonstrated that had direct effect on susceptibility.
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has emerged as a global pandemic, causing 3.5 million deaths until May 1, 2021.1-3 The resurgence of the new mutating variants led to an increase in number infections exacerbating death toll several countries world, making vaccination strategies unworthy.4-7 World health organization (WHO) declares "variant concern" and Indian (Delta variants) are one them.8 In India, second wave started March 2021, by end April, they became first...
Salmonellaenterica serovar Typhi and nontyphoidal Salmonella (NTS) cause the majority of bloodstream infections in sub-Saharan Africa; however, serotyping is rarely performed. We validated a multiplex polymerase chain reaction (PCR) assay with White–Kauffmann–Le Minor (WKLM) scheme using 110 isolates from blood cultures febrile children Ghana applied method other Typhoid Fever Surveillance Africa Program study sites. In Ghana, 47 (43%) S. Typhi, 36 (33%) enterica Typhimurium, 14 (13%)...