A5086367586- Animal Genetics and Reproduction
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Circadian rhythm and melatonin
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Research and Treatments
- Molecular Sensors and Ion Detection
- NF-κB Signaling Pathways
- Neurobiology and Insect Physiology Research
- Plant Disease Resistance and Genetics
- Digestive system and related health
- Autophagy in Disease and Therapy
- Genetics, Aging, and Longevity in Model Organisms
- Yeasts and Rust Fungi Studies
- CRISPR and Genetic Engineering
- Supramolecular Chemistry and Complexes
- Cell death mechanisms and regulation
- Animal Behavior and Reproduction
- Cytomegalovirus and herpesvirus research
- Xenotransplantation and immune response
- Microbial infections and disease research
- Plant Molecular Biology Research
- Synthesis and Characterization of Heterocyclic Compounds
- Wheat and Barley Genetics and Pathology
- RNA regulation and disease
China Agricultural University
2008-2021
Alphaherpesviruses that establish persistent infections rely partly on their ability to evade host antiviral responses, notably the type I interferon (IFN) response. However, mechanisms employed by alphaherpesviruses avoid this response are not well understood. Pseudorabies virus (PRV) is an economically important pathogen and a useful model system for studying alphaherpesvirus biology. To identify PRV proteins antagonize IFN signaling, we performed screen using IFN-stimulated element...
Anion transmembrane transport mediated by novel noncovalent interactions is of central interest in supramolecular chemistry. In this work, a series oxacalix[2]arene[2]triazine-derived transporters 1 and 2 bearing anion−π-, hydrogen-, halogen-bonding sites rational proximity were designed synthesized one-pot strategy starting from gallic acid ester derivatives mono- or di-halogen-substituted triazines. 1H NMR titrations demonstrated efficient binding toward Cl– Br– solution, giving...
Improvement of the nutritional value cow milk with transgenic expression recombinant human α-lactalbumin (α-LA) has been previously attempted. However, detailed characterization protein and analysis components are not explored yet. Here, we first report production healthy cows by somatic cell nuclear transfer, in which up to 1.55 g/L α-LA was achieved. The purified from displayed physicochemical properties similar its natural counterpart respect molecular weight, structure, regulatory...
Abstract Circadian rhythms in behaviour and physiology exist widely animals, plants, fungi cyanobacteria. Although much work has been carried out to characterize the endogenous clock circuit, output signals coupling circadian pacemaker remain elusive. Here, we show that neuropeptide F ( NPF ), a homologue of mammalian Y , its G protein‐coupled receptor NPFR1 regulate locomotor rhythm D rosophila melanogaster . Flies with loss function or were unable ramp up their activity before lights off...
A recombinant fowlpox virus vaccine expressing key protective Mycoplasma gallisepticum antigens could facilitate in the prevention both of and M. infections. Vectormune FP-MG vaccine, a 40k mgc genes, was assessed for its safety 8-wk-old specific-pathogen-free White Leghorn chickens. The serially passaged 5 times by wing-web inoculation. Based on postinoculation clinical observation, gross pathological examination air sacs peritoneum, genetic stability evaluation, shedding tissue...
Abstract Growing evidence indicates that microRNAs play numerous important roles. However, the roles of some involved in regulation circadian rhythm and sleep are still not well understood. In this study, we show miR‐276b is essential for maintaining both by targeting tim , npfr1 DopR1 genes, with deleted mutant flies sleeping more, vice versa overexpressing flies. Through analysing its promoter, found mir‐276b responsive to CLOCK regulates through negative feedback loop CLK/CYC‐TIM/PER....
The small intestine, a digestive organ, supports the body’s immune system. Hence, improving digestion, absorption, and immunological disease resistance levels of intestine will directly contribute to development livestock industry. Previous research has demonstrated that IGF1 could promote but it faces shortage phenotypes were different in transgenic mice attributed multiple transcripts, splice forms, promoters. modification promoter using small-intestine-specific regulatory element binding...
Anti-CD20 monoclonal antibody (mAb, Rituxan) is widely used for the treatment of autoimmune diseases such as B-cell lymphomas. However, production capacity Rituxan currently very limited. Here we report expression recombinant anti-CD20 mAb in milk transgenic cattle, which demonstrated high yield (6.8 mg/mL), purity (>99%), and recovery rate (80%). It revealed that was structurally identical to Rituxan, with exception N-linked glycosylation, mainly afucosylated glycans one degree fucosylation...