A5011099905- Cancer Cells and Metastasis
- Protein Degradation and Inhibitors
- Multiple Myeloma Research and Treatments
- Cell Image Analysis Techniques
- Computational Drug Discovery Methods
- HER2/EGFR in Cancer Research
- HIV/AIDS drug development and treatment
- Neuroblastoma Research and Treatments
- Acute Myeloid Leukemia Research
- Cleft Lip and Palate Research
- Craniofacial Disorders and Treatments
- dental development and anomalies
- Drug Transport and Resistance Mechanisms
- Developmental Biology and Gene Regulation
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Video Surveillance and Tracking Methods
- Histone Deacetylase Inhibitors Research
- Molecular Biology Techniques and Applications
- Hedgehog Signaling Pathway Studies
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Sperm and Testicular Function
Daiichi Sankyo (United States)
2024-2025
Weifang Medical University
2016-2023
AbbVie (United States)
2019-2023
The University of Texas MD Anderson Cancer Center
2023
St. John's University
2020
Bioscience (China)
2019
Weifang University
2019
Shandong Agricultural University
2006-2017
University of Colorado Denver
2009-2017
Third People's Hospital of Hangzhou
2017
We describe functional tests and molecular modeling of erythroid Kruppel-like factor (EKLF) interactions with its DNA binding site. EKLF, a zinc finger-containing, erythroid-specific transcription factor, binds transactivates from the CACCC element, an evolutionarily conserved sequence present within large number promoters enhancers. This element is site naturally occurring point mutations that give rise to beta-thalassemia. have directly tested whether CAC (including two beta-thalassemia...
Normal stem cells from a variety of tissues display unique metabolic properties compared to their more differentiated progeny. However, relatively little is known about cancer cells, also called tumor initiating (TICs). In this study we show that, analogous some normal breast TICs have distinct nontumorigenic (NTCs). Transcriptome profiling using RNA-Seq revealed underexpress genes involved in mitochondrial biology and oxidative phosphorylation, analyses preferentially perform glycolysis...
The actin-binding protein calponin has been implicated in the regulation of smooth muscle contraction. We have isolated cDNA clones encoding a novel acidic isoform from rat aortic vascular cells. initial 273 residues deduced 330 amino acid polypeptide (M(r) 36,377) are highly homologous to basic isoforms, but remaining 57 at carboxyl terminus comprise unique and strongly domain. sequence domain shows high homology (93.3% identity) partial HUMXT01244, an unidentified human hippocampal gene...
Abstract Background Tumors comprise a complex microenvironment of interacting malignant and stromal cell types. Much our understanding the tumor comes from in vitro studies isolating interactions between cells single type, often along pathway. Result To develop deeper within human lung tumors, we perform RNA-seq profiling flow-sorted cells, endothelial immune fibroblasts, bulk freshly resected primary non-small-cell tumors. We map cell-specific differential expression prognostically...
Accurate survival stratification in early-stage non–small cell lung cancer (NSCLC) could inform the use of adjuvant therapy. We developed a clinically implementable mortality risk score incorporating distinct tumor microenvironmental gene expression signatures and clinical variables. Gene profiles from 1106 nonsquamous NSCLCs were used for generation internal validation nine-gene molecular prognostic index (MPI). A quantitative polymerase chain reaction (qPCR) assay was validated on an...
The regulatory mechanisms underpinning facial development are conserved between diverse species. Therefore, results from model systems provide insight into the genetic causes of human craniofacial defects. Previously, we generated a comprehensive dataset examining gene expression during and fusion mouse prominences. Here, used this resource to identify genes that have dynamic patterns in prominences, but for which only limited information exists concerning developmental function.This set ∼80...
Prostate cancer (PCa) is one of the most common malignancies in men globally. The aim present study was to identify key genes and pathways involved occurrence PCa. Gene expression profile (GSE55945) downloaded from Expression Omnibus, differentially expressed (DEGs) were identified. Subsequently, ontology analysis, KEGG pathway analysis protein‑protein interaction (PPI) DEGs performed. Finally, identified confirmed by immunohistochemistry. GO results showed that mainly participated cell...
ABCB1 overexpression is known to contribute multidrug resistance (MDR) in cancers. Therefore, it critical find effective drugs target and overcome MDR. Erdafitinib a tyrosine kinase inhibitor (TKI) of fibroblast growth factor receptor (FGFR) that approved by the FDA treat urothelial carcinoma. Previous studies have demonstrated some TKIs exhibit MDR reversal effect. In this work, we examined whether erdafitinib could reverse mediated ABCB1. The results experiments showed remarkably reversed...
Prostate cancer (PCa) is one of the most common malignancies in men. Ribosomal protein L22-like1 (RPL22L1), a component ribosomal 60 S subunit, associated with progression, but role and potential mechanism RPL22L1 PCa remain unclear. The aim this study was to investigate progression mechanisms involved. Bioinformatics immunohistochemistry analysis showed that expression significantly higher tissues than normal prostate tissues. cell function revealed promoted proliferation, migration...
Using a well-in-drop (WID) oocyte/embryo culture system that allows identification of follicular origin, we have investigated the effects granulosa cells (GCs) apoptosis, follicle size, cumulus–oocyte complexes (COCs) morphology, and cumulus expansion on developmental competence goat oocytes matured cultured individually following parthenogenetic activation. The WID supported oocyte maturation embryo development to level similar conventional group system. majority acquired for up 8–16 cell...
Orofacial malformations resulting from genetic and/or environmental causes are frequent human birth defects yet their etiology is often unclear because of insufficient information concerning the molecular, cellular and morphogenetic processes responsible for normal facial development. We have, therefore, derived a comprehensive expression dataset mouse orofacial development, interrogating three distinct regions - mandibular, maxillary frontonasal prominences. To capture dynamic changes in...
Activating protein 2alpha (AP-2alpha) is known to be expressed in the retina, and AP-2alpha-null mice exhibit defects developing optic cup, including patterning of neural retina (NR) a replacement dorsal retinal pigmented epithelium (RPE) with NR. In this study, we analyzed temporal spatial expression patterns AP-2alpha created conditional deletion retina. exhibited distinct pattern inner nuclear layer colocalization studies indicated that was exclusively postmitotic amacrine cell...
The profusion of high-throughput instruments and the explosion new results in scientific literature, particularly molecular biomedicine, is both a blessing curse to bench researcher. Even knowledgeable experienced scientists can benefit from computational tools that help navigate this vast rapidly evolving terrain. In paper, we describe novel approach challenge, knowledge-based system combines reading, reasoning, reporting methods facilitate analysis experimental data. Reading extract...
Abstract Failure of facial prominence fusion causes cleft lip and palate (CL/P), a common human birth defect. Currently there are two major hypotheses to explain occurrence. The first holds that the epithelium at these locations is somehow defective for while second prominences dysplastic unable appose fuse. Here, using geometric morphometrics, we analyzed outgrowth shape change over time in novel mouse model exhibiting fully penetrant bilateral CL/P. This robust based upon mutations Tfap2a,...
Preeclampsia (PE) is one of the leading causes maternal and perinatal mortality morbidity. One main hallmarks observed in PE impaired inflammation state. In current study, we found that miR-125b was deregulated placental tissues plasma derived from patients, which suggest a potential association between this miRNA pathogenesis PE. Overexpression significantly reduced SGPL1 expression, luciferase assays confirmed direct target miR-125b. We also enhanced IL-8 production by directly targeting...