A5022729081- Hemoglobin structure and function
- Cancer, Hypoxia, and Metabolism
- Blood properties and coagulation
- 3D Printing in Biomedical Research
- Erythrocyte Function and Pathophysiology
- Nanoplatforms for cancer theranostics
- Reproductive Biology and Fertility
- Nitric Oxide and Endothelin Effects
- Analytical Chemistry and Sensors
- Traumatic Brain Injury and Neurovascular Disturbances
- Optical Imaging and Spectroscopy Techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Photodynamic Therapy Research Studies
- Liver Disease and Transplantation
- Neonatal Health and Biochemistry
- Ovarian cancer diagnosis and treatment
- Gastrointestinal disorders and treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Microfluidic and Capillary Electrophoresis Applications
- Immunotherapy and Immune Responses
- Spectroscopy Techniques in Biomedical and Chemical Research
- Dermatology and Skin Diseases
- Advancements in Transdermal Drug Delivery
- Digital Imaging for Blood Diseases
- Diabetic Foot Ulcer Assessment and Management
Keio University
2014-2024
Massachusetts General Hospital
2008-2014
Harvard University
2008-2014
Kawasaki Medical School
2003-2004
When erythrocytes are exposed to hypoxia, hemoglobin (Hb) stabilizes in the T-state by capturing 2,3-bisphosphoglycerate. This process could reduce intracellular pool of glycolytic substrates, jeopardizing cellular energetics. Recent observations suggest that hypoxia-induced activation enzymes is correlated with their release from Band III (BIII) on cell membrane. Based these data, we developed a mathematical model erythrocyte metabolism and compared differences predicted activities enzymes,...
Safe and effective immunologic adjuvants are often essential for vaccines. However, the choice of adjuvant licensed vaccines is limited, especially those that administered intradermally. We show non-tissue damaging, near-infrared (NIR) laser light given in short exposures to small areas skin, without use additional chemical or biological agents, significantly increases immune responses intradermal influenza vaccination augmenting IgE. The NIR laser-adjuvanted vaccine confers increased...
Minimally invasive medical operations, especially endoscope have attracted much attention and play a major role in modern medicine.
To elucidate the function of microcirculation system, it is very important to know blood flow velocity and its distribution in microvessels. We have developed an automated system for measuring by image correlation. The `window' correlation method equivalent sensors various other measurement methods. performed simulations with virtual images consisting random dots before actual ones, examined optimum window shape size. found that reducing size a circular erythrocytes we could measure vivo...
Abstract Brief exposure of skin to near-infrared (NIR) laser light has been shown augment the immune response intradermal vaccination and thus act as an immunologic adjuvant. Although evidence indicates that NIR adjuvant capacity activate innate subsets including dendritic cells (DCs) in conventional adjuvants do, precise immunological mechanism by which acts is largely unknown. In this study we sought identify cellular target using established mouse model influenza examining alteration...
Abstract Except for histological study, there are currently no suitable techniques available the detection and identification of primordial follicles in ovary primary ovarian insufficiency patients who have undetectable AMH levels. Also, ability to locate quantify on cortex strips, without fixation, is valuable could undergo subsequent successful tissue transplantation. Although optical coherence tomography (OCT) a well-established high resolution imaging technique fixation commonly applied...
Abstract The treatment of skin with a low-power continuous-wave (CW) near-infrared (NIR) laser prior to vaccination is an emerging strategy augment the immune response intradermal vaccine, potentially substituting for chemical adjuvant, which has been linked adverse effects vaccines. This approach proved be low cost, simple, small, and readily translatable compared previously explored pulsed-wave medical lasers. However, little known on mode laser–tissue interaction eliciting adjuvant...
Photobiomodulation is a well-established therapeutic modality. However, the mechanism of action poorly understood, due to lack research in causal relationship between near-infrared (NIR) light irradiation and its specific biological effects, hindering broader applications this technology.Since chromophores typically show several absorption peaks, we determined whether effects photobiomodulation are induced with combination two wavelengths at certain range irradiance only, rather than single...
Abstract As oral or intestinal bacteria have been found in pancreatic cystic fluid and tumors, understanding bacterial migration from the duodenum into pancreas via hepato-pancreatic duct is critical. Mathematical models of aerobic to with tumors were developed. Additionally, distributions under pH gradient those flow measured double-layer based microfluidic device T-shaped cylinders. Migration counteracted by bile juice but facilitated pH-taxis acidic toward more favorable slightly alkaline...
Because the regulation of microcirculation in cerebral cortex cannot be analyzed without measuring blood flow dynamics and oxygen concentration microvessels, we developed a fluorescence phosphorescence system for estimating red cell velocity tension noninvasively continuously with high spatial resolution. Using cells labeled fluorescent isothiocyanate to visualize distribution using quenching Pd-meso-tetra-(4-carboxyphenyl)-porphyrin measure enabled simultaneous measurement tension. We...
Hypoxia-inducible factor is a hypoxia-responsive transcriptional that controls the expression of proteins contributing to homeostatic responses hypoxia. Spatial heterogeneity tissue oxygenation has been postulated as determinant structure and function hepatic lobules, although its molecular mechanisms remain unknown. This study aimed examine role HIF-1 expressed in hepatocytes regulation microcirculation.We have generated mice harboring floxed HIF-1α allele, employed albumin-Cre transgenic...
The purpose of this study was to evaluate the possible clinical application optical coherence tomography for assessing ovarian reserve in individual specimens human tissue fertility preservation.Ovarian examination by performed before cryopreservation. Three four subjects had hematological disease or cancer, and they faced a threat their due impending chemotherapy. One patient underwent extraction vitro activation dormant follicles as treatment.The current full-field technique can detect...
Efficient and timely delivery of vaccine antigens to the secondary lymphoid tissue is crucial induce protective immune responses by vaccination. However, determining longitudinal biodistribution injected vaccines in body has been a challenge. Here, near-infrared (NIR) fluorescence imaging reported that can efficiently enable trafficking real time. Zwitterionic NIR fluorophores are conjugated on surface model tracked fate bioconjugated after intradermal administration. Using an system, it...