A5047064091- Veterinary Oncology Research
- Virus-based gene therapy research
- Ultrasound and Hyperthermia Applications
- Infectious Diseases and Mycology
- Sarcoma Diagnosis and Treatment
- Oral and Maxillofacial Pathology
- Salivary Gland Tumors Diagnosis and Treatment
- Microbial Inactivation Methods
- Cancer Research and Treatments
- Tendon Structure and Treatment
- Fibroblast Growth Factor Research
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Microfluidic and Bio-sensing Technologies
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- Pulsed Power Technology Applications
- Microbial infections and disease research
- Cancer Cells and Metastasis
- Abdominal Surgery and Complications
- Hedgehog Signaling Pathway Studies
- Craniofacial Disorders and Treatments
- Genomics and Phylogenetic Studies
- Veterinary Orthopedics and Neurology
- Ultrasound Imaging and Elastography
Virginia–Maryland College of Veterinary Medicine
2019-2024
Virginia Tech
2022-2024
University of Wisconsin–Madison
2022
Colorado State University
2009-2021
Purdue University West Lafayette
2020
North Carolina State University
2014-2019
Pfizer (Ireland)
2016
To evaluate the outcome in terms of progression-free interval (PFI) and overall survival time (ST) after curative-intent resection oral melanoma dogs.Retrospective case series.70 client-owned dogs.An electronic medical record search review was performed for dogs that underwent (May 1, 1998, to December 31, 2011). Information gathered included signalment, location tumor, staging results, type surgery, adjuvant therapy, findings on histologic evaluation, outcome.36 (51.4%), 16 (22.9%), 13...
Abstract Objective —To evaluate the relationship between width and depth of surgical margins, amount edema within around tumor, degree demarcation tumor surrounding tissues with clinical outcome following removal cutaneous mast cell tumors (cMCTs) in dogs. Design —Retrospective cohort study. Animals —100 dogs 115 resectable cMCTs. Procedures —Information about dogs' outcomes cMCT was obtained from primary care veterinarians. Histologic sections excised were assessed retrospectively for grade...
In this study, the feasibility of treating canine primary lung tumors with high-frequency irreversible electroporation (H-FIRE) was investigated as a novel cancer treatment option. H-FIRE is minimally invasive tissue ablation modality that delivers bipolar pulsed electric fields to targeted cells, generating nanopores in cell membranes and rendering cells nonviable. current patients (n = 5) underwent an applied voltage 2250 V using 2-5-2 µs waveform achieve partial tumor prior surgical...
Abstract Osteosarcoma (OS) is the most common primary bone tumor in dogs and humans, similarities between human canine OS make dog a valuable comparative oncology model. Histotripsy, non-invasive ablation modality using pulsed focused ultrasound to mechanically disintegrate tissue, shows promise for treatment of tumors. Previous clinical trials have shown safety feasibility ablating tumors, followed by limb amputation. The present study first use histotripsy without standard-of-care...
Abstract Background: Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents, associated with poor prognosis, especially cases metastatic disease at diagnosis. Canine OS shares many biological clinical similarities human OS, making dogs valuable comparative models for research. Histotripsy a non-thermal, non-invasive high-intensity focused ultrasound modality that mechanically disrupts cells, triggering release of neoantigens damage-associated molecular...
To provide updated information on the distribution of histopathologic types primary pulmonary neoplasia in dogs and evaluate effect postoperative adjuvant chemotherapy with carcinoma.340 dogs.Medical records that underwent lung lobectomy for removal a mass were reviewed, type lesions was determined. The canine carcinoma stage classification system used to determine clinical carcinoma.Pulmonary most frequently encountered tumor (296/340 [87.1%]), followed by sarcoma (26 [7.6%]), adenoma (11...
<italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Introduction:</i> Histotripsy is a non-invasive focused ultrasound therapy that uses controlled acoustic cavitation to mechanically disintegrate tissue. To date, there are no reports investigating histotripsy for the treatment of soft tissue sarcoma (STS). xmlns:xlink="http://www.w3.org/1999/xlink">Objective:</i> This study aimed investigate xmlns:xlink="http://www.w3.org/1999/xlink">in vivo</i>...
Abstract Despite a considerable expenditure of time and resources significant advances in experimental models disease, cancer research continues to suffer from extremely low success rates translating preclinical discoveries into clinical practice. The continued failure drug development, particularly late the course human testing, not only impacts patient outcomes, but also drives up cost for those therapies that do succeed. It is clear paradigm shift necessary if improvements this process...
Objective To report demographic characteristics of a contemporary population dogs with appendicular osteosarcoma and assess the relationship between characteristics, site distribution, phylogenetic breed clusters. Design Retrospective case series. Methods A search Veterinary Medical Database was performed for as new diagnosis. Entries were reviewed sex, neuter status, age at diagnosis, breed, affected limb, tumor location. The reported purebred used to categorize each dog into one five...
<italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Objective:</i> Osteosarcoma (OS) is a devastating primary bone tumor in dogs and humans with limited non-surgical treatment options. As the first completely non-invasive non-thermal ablation technique, histotripsy has potential to significantly improve standard of care for patients tumors. xmlns:xlink="http://www.w3.org/1999/xlink">Introduction:</i> Standard appendicular OS involves surgical...
Abstract Objective —To evaluate the outcome of resection simultaneous discrete bilateral mobile thyroid gland carcinomas (TGCs) in dogs. Design —Retrospective case series. Animals —15 dogs with resected TGCs. Procedures —Medical records (from 1994 to 2010) were searched for appropriate diagnosis and treatment. Information collected included signalment, clinical signs, diagnostic test results, tumor mobility (mobile identified by movement ≥ 1 cm all planes during palpation), complications,...
A three-step commercial manufacturing route has been developed for palbociclib, a highly selective, reversible inhibitor of CDK 4/6. The second step, which utilizes Heck coupling to install the enol ether side chain, is described. regioselective catalyst was identified this transformation along with reaction conditions that ensure robustness upon scale-up. Effective removal palladium accomplished via filtration insoluble metal and an extractive chelation step. Finally, efficient isolation...
Abstract Background Lower urinary tract transitional cell carcinoma (TCC) is an important but rarely described disease of cats. Objectives To report the clinical characteristics, treatments, and outcomes in a cohort cats with lower TCC to test identified variables for prognostic relevance. Animals One‐hundred eighteen client‐owned carcinoma. Methods Medical records were retrospectively reviewed obtain information regarding outcomes. Recorded analyzed statistically. Results Median age...
Report clinical outcomes of dogs with surgically excised mast cell tumors (MCT) and soft tissue sarcomas (STS).Prospective study.Fifty-three 52 MCT (50 low grade, 2 high grade) 19 STS (12 grade I, 6 II, 1 III).All were examined at 3, 6, 12, 18, 24 months postoperatively, cytologic or histopathologic evaluation suspected local recurrences. Dogs euthanized because study tumor-related causes underwent necropsy.Median intraoperative margins 20 mm 30 wide for STS, respectively, fascial plane...
Abstract New therapies to treat pancreatic cancer are direly needed. However, efficacious interventions lack a strong preclinical model that can recapitulate patients’ anatomy and physiology. Likewise, the availability of human primary malignant tissue for ex vivo studies is limited. These significant limitations in biomedical device field. We have developed RAG2/IL2RG deficient pigs using CRISPR/Cas9 as large animal with novel application xenograft adenocarcinoma. In this proof-of-concept...
Osteosarcoma (OS) is a malignant bone tumor treated by limb amputation or salvage surgeries and chemotherapy. Histotripsy non-thermal, non-invasive focused ultrasound therapy using controlled acoustic cavitation to mechanically disintegrate tissue. Recent ex vivo in pilot studies have demonstrated the ability of histotripsy for ablating OS but were limited scope. This study expands on these initial findings more fully characterize effects tumors, particularly tumors with different...
Background: Osteosarcoma (OS) is the most frequently occurring malignant bone tumor in humans, primarily affecting children and adolescents. Significant advancements treatment options for OS have not occurred last several decades, prognosis remains grim with only a 70% rate of 5-year survival. The objective this study was to investigate focused ultrasound technique histotripsy as novel, noninvasive option OS. Methods: We utilized heterotopic murine model establish feasibility ablating tumors...
Monocytes/macrophages are likely key cells in immune modulation dogs with osteosarcoma (OSA). Increased peripheral monocyte counts negatively correlated shorter disease-free intervals OSA. Understanding the monocyte/macrophage's modulatory role OSA can direct further studies immunotherapy development for OSA.That evades response by down-regulating chemokine receptor expression and migratory function, suppresses host responses.Eighteen that have not received definitive treatment 14 healthy...
Abstract Since William Coley utilized bacterial immunotherapy to treat sarcomas in the late 19th century, an association between infection and improved survival has been reported for human canine osteosarcoma patients. One of reasons this is likely a reactivation host immune system towards inflammatory anti‐tumour response, one key players macrophage. Yet, despite their importance, response macrophages infectious agents context not thoroughly evaluated. The aim study was evaluate how vitro...
Purpose To investigate the safety, feasibility, and outcomes of High-Intensity Focused Ultrasound (HIFU) for treatment solid tumors in a spontaneous canine cancer model.Methods Dogs diagnosed with subcutaneous were recruited, staged pretreatment biopsies obtained. A single HIFU was delivered to result partial tumor ablation using commercially available unit. Tumors resected 3–6 days post samples obtained histopathology immunohistochemistry. Total RNA isolated from paired pre treated FFPE...