A5101536495- Cancer-related molecular mechanisms research
- Platelet Disorders and Treatments
- Glaucoma and retinal disorders
- Blood groups and transfusion
- Retinal Imaging and Analysis
- RNA modifications and cancer
- Digital Imaging for Blood Diseases
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hematopoietic Stem Cell Transplantation
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Ginseng Biological Effects and Applications
- Acute Myeloid Leukemia Research
- Complement system in diseases
- Telomeres, Telomerase, and Senescence
- Helicobacter pylori-related gastroenterology studies
- Case Reports on Hematomas
- Chronic Myeloid Leukemia Treatments
- Berberine and alkaloids research
- Mycobacterium research and diagnosis
- Abdominal Trauma and Injuries
- Polysaccharides and Plant Cell Walls
- RNA regulation and disease
- Blood properties and coagulation
Peking University People's Hospital
2006-2025
Peking University
2006-2025
Shanghai Sixth People's Hospital
2015-2024
Shanghai Jiao Tong University
2019-2024
Zhejiang Chinese Medical University
2024
Third Affiliated Hospital of Zhengzhou University
2024
Zhongnan Hospital of Wuhan University
2019-2023
Wuhan University
2019-2023
Henan University of Technology
2018-2022
Zhengzhou University
2022
We propose a method to locate automatically the optic disc (OD) in fundus images of retina. Based on properties OD, our proposed includes edge detection using Sobel or Canny method, and circles Hough transform. The transform assists center radius circle that approximates margin OD. feature OD is one bright areas image, potential detected by are analyzed intensity. Forty retina from DRIVE database were used evaluate performance method. success rates including both good acceptable detections...
Impaired megakaryocyte (MK) maturation and reduced platelet production are important causes of immune thrombocytopenia (ITP). However, MK distribution bone marrow (BM) niche alteration in ITP unclear. To investigate the MKs BM examine components regulation migration, peripheral blood were obtained from 30 patients 28 healthy donors. Nestin+ mesenchymal stem cells (MSCs) CD41+ sorted by fluorescence-activated cell sorting. The related signaling analyzed via immunofluorescence, flow cytometry,...
The Fe(III) chelating activity of anthocyanin extracted from black soybean coats was investigated at pH 3.0, 5.0, 6.5, 7.0, and 7.4 with fluorescence spectroscopy microscale thermophoresis (MST). Cyanidin-3-glucoside (C3G) determined to be 98% the total by high-performance liquid chromatography. binding affinity (Ka) exhibited significant pH-dependent behavior: Ka 9.7167 × 104, 1.0837 1.4284 5.4550 3.0269 104 M–1 7.4, respectively (p < 0.05). MST data showed that ΔG 0 ΔH 0, demonstrating...
To investigate the changes of retinal nerve fiber layer (RNFL) in patients after an attack primary acute angle closure glaucoma (PAACG) and to assess impact time on prognosis changes.cross-sectional study.Twenty-six with unilateral PAACG cataracts from 2017 2019 were enrolled. Eyes less than 1 day constituted group A (n = 13), while eyes more B 13). All received phacoemulsification viscogoniosynechialysis intraocular pressure (IOP) lowering. underwent ophthalmic examinations including IOP,...
In our previous studies, two polysaccharides (MC-1 and MC-2) were identified in the roots of maca (Lepidium meyenii). this study, effects these on intestinal immunity inflammation assessed using epithelial cells, i.e., Caco-2 cells. The levels cytokines tight junction proteins measured ELISA qPCR, respectively. results showed that MC-1 MC-2 difficult to be absorbed cell monolayer, but promoted secretion IL-8, IL-10, IL-12, INF-γ TNF-α, IL-6, NO macrophages (RAW264.7 cells) via monolayer....
Recent studies have suggested a role for N6-methyladenosine (m6A) modification in neurological diseases. Hypothermia, commonly used treatment traumatic brain injury, plays neuroprotective by altering m6A modifications. In this study, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was applied to conduct genome-wide analysis of methylation the rat hippocampus Sham and injury (TBI) groups. addition, we identified expression mRNA after TBI with hypothermia treatment. Compared group,...
Fundus images of the retina are color eye taken by specially designed digital cameras. Ophthalmologists rely on fundus to diagnose various diseases that affect eye, such as di
Endoplasmic reticulum stress (ERS) in the retinal Müller cells is a key factor contributing to inflammation and vascular leakage diabetic retinopathy (DR). This study was investigate underlying mechanisms through which 3 main unfolded protein response (UPR) pathways regulate ERS examine expression levels of endothelial growth (VEGF) vitro. Rat cell lines were stimulated with high glucose mimic environment PKR-like endoplasmic kinase (PERK), inositol-requiring enzyme 1 (IRE1) activating...
The aim of the present study was to investigate characteristic microRNAs (miRNAs) expressed during pre‑invasive and invasive stages cervical cancer. A gene expression profile (GSE7803) containing 21 squamous cell carcinoma samples, 10 normal epithelium samples seven high‑grade intraepithelial lesion obtained from Gene Expression Omnibus. Differentially genes (DEGs) were identified using significance analysis microarray software, a Ontology (GO) enrichment conducted Database for Annotation,...
Significance Repetitive DNA sequences are abundant in the human genome, and their high variabilities contribute to genetic diversity disease susceptibility. Here, we report that an intronic variable number tandem repeat element, VNTR2-1, is critical for transcription of telomerase reverse transcriptase ( hTERT ) gene a cell-context–dependent manner. Removal this element at its native genomic site cancer cells resulted telomere shortening, cellular senescence, impaired tumor growth. VNTR2-1...
Recent studies showed that RNA N6-methyladenosine (m6A) plays an important role in neurological diseases. We used methylated immunoprecipitation sequencing (MeRIP-Seq) technology to generate the m6A modification map after traumatic spinal cord injury (TSCI). A total of 2,609 differential peaks were identified TSCI. Our results TSCI 4,206 genes with significantly altered expression. Cross-link analysis and 141 hyper-methylated upregulated, 53 downregulated, 57 hypo-methylated 197...
Abstract Ovarian cancer (OV) is a highly fatal malignant disease that commonly manifests at an advanced stage. Drug resistance, particularly platinum leading cause of treatment failure because first-line systemic chemotherapy primarily relies on platinum-based regimens. By analyzing the gene expression levels in Cancer Genome Atlas database, Genotype-Tissue Expression and Gene Omnibus datasets, we discerned HOXB2 was expressed OV associated with poor prognosis cisplatin resistance....