A5059469183- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Advanced Proteomics Techniques and Applications
- Genetic Associations and Epidemiology
- Peroxisome Proliferator-Activated Receptors
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Mass Spectrometry Techniques and Applications
- Genomic variations and chromosomal abnormalities
- Adipose Tissue and Metabolism
- Genomics and Chromatin Dynamics
- Genetic Mapping and Diversity in Plants and Animals
- Regulation of Appetite and Obesity
- Genomics and Rare Diseases
- Gene expression and cancer classification
- Chromosomal and Genetic Variations
- Cancer, Hypoxia, and Metabolism
- Lipoproteins and Cardiovascular Health
- Genetic and phenotypic traits in livestock
- Cancer, Lipids, and Metabolism
- Biochemical Analysis and Sensing Techniques
- Birth, Development, and Health
- Endoplasmic Reticulum Stress and Disease
- RNA and protein synthesis mechanisms
Wake Forest University
2007-2024
Texas Biomedical Research Institute
2013-2022
University of Iowa
2022
University of Maryland, Baltimore
2020
University of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center
2020
Atrium Health Wake Forest Baptist
2019
Medical College of Wisconsin
2006-2018
University of Mississippi Medical Center
2017
Cincinnati Children's Hospital Medical Center
2017
Jackson Memorial Hospital
2017
Comparison of genomic DNA sequences from human and mouse revealed a new apolipoprotein (APO) gene ( APOAV ) located proximal to the well-characterized APOAI/CIII/AIV cluster on 11q23. Mice expressing transgene showed decrease in plasma triglyceride concentrations one-third those control mice; conversely, knockout mice lacking Apoav had four times as much triglycerides controls. In humans, single nucleotide polymorphisms (SNPs) across locus were found be significantly associated with levels...
With the rapid adoption of high-throughput omic approaches to analyze biological samples such as genomics, transcriptomics, proteomics and metabolomics, each analysis can generate tera- peta-byte sized data files on a daily basis. These file sizes, together with differences in nomenclature among these types, make integration multi-dimensional omics into biologically meaningful context challenging. Variously named integrated omics, multi-omics, poly-omics, trans-omics, pan-omics or shortened...
The recently identified apolipoprotein A5 gene (APOA5) has been shown to play an important role in determining plasma triglyceride concentrations humans and mice. We previously APOA5 haplotype (designated APOA5*2) that is present approximately 16% of Caucasians associated with increased concentrations. In this report we describe another (APOA5*3) containing the rare allele single nucleotide polymorphism c.56C>G changes serine tryptophan at codon 19 independently high levels three different...
Polycystic ovary syndrome (PCOS) is a common, highly heritable complex disorder of unknown aetiology characterized by hyperandrogenism, chronic anovulation and defects in glucose homeostasis. Increased luteinizing hormone relative to follicle-stimulating secretion, insulin resistance developmental exposure androgens are hypothesized play causal role PCOS. Here we map common genetic susceptibility loci European ancestry women for the National Institutes Health PCOS phenotype, which confers...
To make large-scale association studies a reality, automated high-throughput methods for genotyping with single-nucleotide polymorphisms (SNPs) are needed. We describe PCR conditions that permit the use of TaqMan or 5′ nuclease allelic discrimination assay typing large numbers individuals any SNP and computational allow genotypes to be assigned automatically. demonstrate utility these methods, we typed >1600 G-to-T transversion results in glutamate-to-aspartate substitution at position...
Since triglycerides (TG) are a major independent risk factor for coronary heart disease, understanding their genetic and environmental determinants is of importance. Mouse models indicate an inverse relationship between levels the newly identified apolipoprotein AV (APOAV) TG concentrations. We have examined relative influence human APOA5 variants on plasma lipids, compared to impact variation in APOC3 APOA4 which lie same cluster. Single nucleotide polymorphisms (SNPs) (S19W, -1131T>C)...
Abstract We have placed 7,600 cytogenetically defined landmarks on the draft sequence of human genome to help with characterization genes altered by gross chromosomal aberrations that cause disease. The are large-insert clones mapped chromosome bands fluorescence in situ hybridization. Each clone contains a tag is positioned genomic sequence. This genome-wide set sequence-anchored allows structural and functional analyses genome. resource represents first comprehensive integration...
Steady-state expression quantitative trait loci (eQTLs) explain only a fraction of disease-associated identified through genome-wide association studies (GWASs), while eQTLs involved in gene-by-environment (GxE) interactions have rarely been characterized humans due to experimental challenges. Using baboon model, we found hundreds that emerge adipose, liver, and muscle after prolonged exposure high dietary fat cholesterol. Diet-responsive exhibit genomic localization genic features are...
Abstract The analysis of mitochondrial DNA sequences has for a long time been the most extensively used genetic tool phylogenetic, phylogeographic and population studies. Since this approach only considers female lineages, it tends to give biased picture history. use protein polymorphisms microsatellites helped obtain more unbiased view, but complementing studies with Y chromosome markers could clarify role each sex in natural processes. In study we analysed variability at four...
Combined hyperlipidemia is a common disorder, characterized by highly atherogenic lipoprotein profile and substantially increased risk of coronary heart disease. The purpose this study was to establish whether variations apolipoprotein A5 (APOA5), newly discovered gene lipid metabolism located 30 kbp downstream the APOA1/C3/A4 cluster, contributes transmission familial combined (FCHL).We performed linkage association tests on 128 families. Two independent alleles, APOA5c.56G APOC3c.386G,...
The growth hormone secretagogue receptor (GHSR) (ghrelin receptor) plays an important role in the regulation of food intake and energy homeostasis. GHSR gene lies on human chromosome 3q26 within a quantitative trait locus strongly linked to multiple phenotypes related obesity metabolic syndrome. Because biological function location make it excellent candidate gene, we tested relation between common single nucleotide polymorphisms (SNPs) obesity. We performed comprehensive analysis SNPs,...
Fatty acid-binding proteins (FABPs) play regulatory roles at the nexus of lipid metabolism and signaling. Dyslipidemia in clinical manifestation frequently co-occurs with obesity, insulin resistance hypertension Metabolic Syndrome (MetS). Animal studies have suggested FABPs expressing MetS phenotypes. In our family cohort Northern European descent, transcript levels peripheral white blood cells (PWBCs) a key FABPs, FABP3, is correlated leading components. However, evidence supporting...
Abstract Mutations of pigment type switching have provided basic insight into melanocortin physiology and evolutionary adaptation. In all vertebrates that been studied to date, two key genes, Agouti Melanocortin 1 receptor (Mc1r), encode a ligand-receptor system controls the switch between synthesis red–yellow pheomelanin vs. black–brown eumelanin. However, in domestic dogs, historical studies based on pedigree segregation analysis suggested type-switching is more complicated fundamentally...
Membrane-bound and membrane-associated proteins are difficult to analyze by mass spectrometry, since the association with lipids impedes isolation solubilization of in buffers suitable for spectrometry efficient generation positively charged peptide ions electrospray ionization. Current methods mostly utilize detergents from membranes. In this study, we present an improved detergent-free method spectrometric identification membrane-bound proteins. We delipidate membrane bilayer chloroform...
Administration of amplitude modulated 27·12 MHz radiofrequency electromagnetic fields (AM RF EMF) by means a spoon-shaped applicator placed on the patient's tongue is newly approved treatment for advanced hepatocellular carcinoma (HCC). The mechanism action tumour-specific AM EMF largely unknown.
Gas chromatography–mass spectrometry (GC-MS) platforms are typically run in electron ionization (EI) mode for mass spectral matching and metabolite annotation. With the advent of high resolution (HRMS), soft techniques such as chemical (CI) may provide additional coverage compound identification. We evaluated NIST SRM 1950 pooled plasma reference sample using a HRGC-MS instrument [GC-Orbitrap-MS with (EI), positive (PCI), negative CI (NCI) capabilities] annotation quantification to assess...
The main goal of comparative proteomics is the quantitation differences in abundance many proteins between two different biological samples a single experiment. By differentially labeling peptides from and combining them analysis, relative ratios protein can be accurately determined. Protease catalyzed (18)O exchange simple method to label peptides, but lack robust software tools analyze data mass spectra labeled generated by common ion trap spectrometers has been limitation. ZoomQuant...