A5014158156- Biochemical effects in animals
- Free Radicals and Antioxidants
- Aldose Reductase and Taurine
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Diabetes and associated disorders
- Adipose Tissue and Metabolism
- Healthcare and Venom Research
- Stress Responses and Cortisol
- Coenzyme Q10 studies and effects
- Chronic Kidney Disease and Diabetes
- Bee Products Chemical Analysis
- Eicosanoids and Hypertension Pharmacology
- Growth Hormone and Insulin-like Growth Factors
- Genetic Syndromes and Imprinting
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Diet and metabolism studies
- Diabetes Treatment and Management
- Biochemical Acid Research Studies
- Regulation of Appetite and Obesity
- Diet, Metabolism, and Disease
- Virus-based gene therapy research
Karolinska Institutet
2011-2022
Atrogi (Sweden)
2020
Stockholm University
2018
Karolinska University Hospital
2011-2015
Background: Excessive production of mitochondrial reactive oxygen species (ROS) is a central mechanism for the development diabetes complications. Recently, hypoxia has been identified to play an additional pathogenic role in diabetes. In this study, we hypothesized that ROS overproduction was secondary impaired responses due inhibition hypoxia-inducible factor-1 (HIF-1) by hyperglycemia. Methods: The levels were analyzed blood healthy subjects and individuals with type 1 after exposure...
In rodents, carnosine treatment improves diabetic nephropathy, whereas little is known about the role and function of anserine, methylated form carnosine. Antioxidant activity was measured by oxygen radical absorbance capacity stress response in human renal tubular cells (HK-2) RT-PCR Western-Immunoblotting. wildtype (WT) mice (db/db), effect short-term anserine on blood glucose, proteinuria vascular permeability measured. Anserine has a higher antioxidant compared to (p < 0.001). stressed...
Chronic stimulation of β2-adrenoceptors, opposite to acute treatment, was reported reduce blood glucose levels, as well improve and insulin tolerance in rodent models diabetes by essentially unknown mechanisms. We recently described a novel pathway that mediates uptake skeletal muscle cells via β2-adrenoceptors. In the current study we further explored potential therapeutic relevance β2-adrenoceptor homeostasis mechanisms responsible for effect.C57Bl/6N mice with diet-induced obesity were...
We have investigated promoter methylation of the Insr, Igf1 and Igf1r genes in skeletal cardiac muscles normal diabetic db/db mice. No differences Insr were found heart no was detected muscle. In muscle, males exhibited a 7.4-fold increase methylation, which accompanied by 1.8-fold decrease mRNA levels, compared with controls. More than 50% events concentrated within an 18 bp sequence that includes one Sp1 binding sites. conclude level pattern muscle is related to gender state.
IGF binding protein 1 (IGFBP1) is a member of the proteins for with an important role in glucose homeostasis. Circulating IGFBP1 derived essentially from liver where it mainly regulated negatively by insulin. Carnosine, natural antioxidant, has been shown to improve metabolic control different animal models diabetes but its mechanisms action are still not completely unraveled. We therefore investigate effect carnosine treatment on regulation db/db mice. Db/db mice and heterozygous...
In humans, low serum carnosinase (CN1) activity protects patients with type 2 diabetes from diabetic nephropathy. We now characterized the interaction of thiol-containing compounds CN1 cysteine residue at position 102, which is important for activity. Reduced glutathione (GSH), N-acetylcysteine and (3.2 ± 0.4, 2.0 0.3, 1.6 0.2 µmol/mg/h/mM; p < .05) lowered dose-dependently recombinant (rCN1) efficiency (5.2 µmol/mg/h/mM) normalized increased renal tissue samples mice. Inhibition was...
Mitochondrial dysfunction in type 2 diabetes leads to oxidative stress, which drives disease progression and complications. L-carnosine, an endogenous dipeptide, improves metabolic control, wound healing kidney function animal models of diabetes. Coenzyme Q (CoQ), a component the mitochondrial electron transport chain, possesses similar protective effects on We aimed study effect carnosine CoQ, assess any synergistic CoQ improved mouse model Carnosine enhanced gene expression increased...
<i>Background/Aims:</i> Vasopeptidase as an agent inhibits membrane metalloendopeptidase (MME, also known neutral endopeptidase). MME is widely distributed in the body and particularly abundant kidney. The <i>MME </i>gene located on chromosome 3q25.1 within a linkage region for diabetic nephropathy (DN). present study aims to evaluate genetic functional effects of development DN. <i>Methods:</i> A case-control the<i> MME</i> gene type 1...
Abstract Background Excessive production of mitochondrial reactive oxygen species (ROS) is a central mechanism for the development diabetes complications. Recently, hypoxia has been identified to play an additional pathogenic role in diabetes. In this study, we hypothesized that ROS overproduction was secondary impaired responses due inhibition hypoxia-inducible factor-1 (HIF-1) by hyperglycemia. Methods The dynamic levels analysed blood healthy subjects and individuals with type 1 after...