A5007380847- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Lymphoma Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Lymphatic System and Diseases
- Acute Myeloid Leukemia Research
- Immune cells in cancer
- Chronic Lymphocytic Leukemia Research
- Inflammatory Biomarkers in Disease Prognosis
- Angiogenesis and VEGF in Cancer
- Colorectal Cancer Surgical Treatments
- Lung Cancer Diagnosis and Treatment
- Vascular Malformations Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Electrospun Nanofibers in Biomedical Applications
- CAR-T cell therapy research
- Pain Mechanisms and Treatments
- Colorectal and Anal Carcinomas
- Acute Lymphoblastic Leukemia research
- Dermatological and Skeletal Disorders
- Ferroptosis and cancer prognosis
- Cancer Cells and Metastasis
- Histone Deacetylase Inhibitors Research
- Vascular Malformations and Hemangiomas
- Protein Degradation and Inhibitors
University of Parma
2014-2024
University of Brescia
2010
University of Florence
1993-1994
Purpose: The success of immune checkpoint inhibitors strengthens the notion that tumor growth and regression are regulated. To determine whether distinct tissue microenvironments differentially affect clinical outcome in non-small cell lung cancer (NSCLC), an extended analysis PD-L1 tumor-infiltrating lymphocytes (TIL) was performed.Experimental Design: Samples from resected adenocarcinoma (ADC 42), squamous carcinoma (SCC 58), 26 advanced diseases (13 ADC 13 SCC) treated with nivolumab were...
NOTCH1 PEST domain mutations are often seen in hematopoietic malignancies, including T-cell acute lymphoblastic leukemia (T-ALL), chronic lymphocytic (CLL), splenic marginal zone lymphoma (SMZL), mantle cell (MCL), and diffuse large B-cell (DLBCL). These play a key role the development progression of lymphoproliferative tumors by increasing Notch signaling and, consequently, promoting proliferation, survival, migration, suppressing apoptosis. There is currently no specific treatment...
Diets in which fat is significantly provided by olive oil and are relatively rich vegetables, have been associated with a low incidence of cardiovascular diseases, mostly due to the presence several phenolic compounds anti-oxidant antiinflammatory properties. [1]. In this work, we describe anti-inflammatory effect 3,4-DHPEA-EDA cell model that developed mimic inflammatory injury endothelium. This was based on production proinflammatory chemokine CCL2, following vitro stimulation primary...
Upon tissue injury, peripheral sensory neurons release nociceptive factors (e.g. substance P [SP]), which exert local and systemic actions including the recruitment of bone marrow (BM)-derived haematopoietic stem progenitor cells (HSPCs) endowed with paracrine pro-angiogenic properties. We herein explore whether diabetic neuropathy interferes these phenomena. first investigated presence in BM patients type 2 diabetes by immunohistochemistry morphometry analyses nerve size density assessment...
Endothelial cells (ECs) are a site of human cytomegalovirus (HCMV) productive replication, haematogenous dissemination and persistence, assumed to play critical role in the development HCMV-associated vascular diseases. Although early reports have shown presence HCMV antigens DNA lymphoid tissues, ability infect lymphatic ECs (LECs) has remained unaddressed due lack suitable vitro system. This study provided evidence that clinical isolate (retaining its natural endotheliotropism) was able...
Genomic studies have identified recurrent somatic alterations in genes involved DNA methylation and post-translational histone modifications acute lymphoblastic leukemia (ALL), suggesting new opportunities for therapeutic interventions. In this study, we G9a/EHMT2 as a potential target T-ALL through the intersection of epigenome-centered shRNA chemical screens. We subsequently validated G9a with low-throughput CRISPR-Cas9-based targeting catalytic SET-domain testing inhibitors vitro, 3D,...
The mechanisms underlying the success of propranolol in treatment infantile hemangioma (IH) remain elusive and do not fully explain rapid regression hemangiomatous lesions following drug administration. As autophagy is critically implicated vascular homeostasis, we determined whether β-blockers trigger autophagic flux on hemangioma-derived endothelial cells (Hem-ECs) vitro. Fresh tissue specimens, surgically removed for therapeutic purpose to seven children affected by proliferative IH, were...
We determined whether sex-associated tissue and blood immune background characterizes surgically resected NSCLC advanced patients undergoing immunotherapy (IO), potentially affecting clinical outcome. Peripheral blood, collected at surgery from stage I-III, baseline first disease assessment (T1) (chemo)-IO treated NSCLC, was subjected to FACS analysis of multiple cellular immunophenotypic functional properties multiplex ELISA assay measure serum cytokines. Tumor Immune Microenvironment...
Abstract Purpose: To uncover the underpinnings of acquired resistance (AR) to immunotherapy (IO), we determined whether distinctive clinico-pathological, radiomic and peripheral blood (PB) immune-inflammatory features reflect oligo- systemic (sys)-AR in advanced NSCLC patients undergoing immune checkpoints inhibitors. Experimental Design: On 105 consecutive IO-treated NSCLC, PB immunophenotypes, cytokines CT-derived (RFs), extracted from primary merged metastatic lesions, were prospectively...
Lymphangiogenesis plays a critical role in the immune response, tumour progression and therapy effectiveness. The aim of this study was to determine whether interplay between lymphatic blood microvasculature, tumour-infiltrating lymphocytes programmed death 1 (PD-1)/programmed ligand (PD-L1) checkpoint constitutes an microenvironment affecting clinical outcome patients with non-small-cell lung cancer.Samples from 50 squamous cell carcinomas 42 adenocarcinomas were subjected...
OBJECTIVESThe aim of the present study was to characterize biological properties and in vivo tumourigenic potential mesenchymal cells (MCs) obtained from non-small-cell lung cancer (NSCLC) samples.
Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate role in normal and diseased lung. We describe a simple inexpensive method harvest, isolate, expand human lung (HL-LECs). Fifty-five samples healthy selected patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies CD31 Podoplanin, was employed select pure population HL-LECs. The purity HL-LECs assessed by...
The immune regulation of cancer growth and regression has been underscored by the recent success immunotherapy. possibility that microenvironmental factors may impact on clinical outcome treatment response still requires intense investigations. Hereby, supporting data research article "Integrated CT Imaging Tissue Immune Features Disclose a Radio-Immune Signature with High Prognostic Impact Surgically Resected NSCLC" [1], are presented. With ultimate aim to provide non-invasive prognostic...
Radiomics has emerged as a noninvasive tool endowed with the potential to intercept tumor characteristics thereby predicting clinical outcome. In recent study on resected non-small cell lung cancer (NSCLC), we identified highly prognostic computed tomography (CT) -derived radiomic features (RFs), which in turn were able discriminate hot from cold immune microenvironment (TIME). We aimed at validating model capable of dissecting specific TIME profiles bearing power NSCLC. The validation...
The aim of the present study was to dissect clinical outcome GB patients through integration molecular, immunophenotypic and MR imaging features.We enrolled 57 histologically proven molecularly tested (5.3% IDH-1 mutant). Two-Dimensional Free ROI on Biggest Enhancing Tumoral Diameter (TDFRBETD) acquired by MRI sequences were used perform a manual evaluation multiple quantitative variables, among which we selected: SD Fluid Attenuated Inversion Recovery (FLAIR), mean Apparent Diffusion...
Infantile hemangiomas (IHs) are benign vascular neoplasms of childhood (prevalence 5–10%) due to the abnormal proliferation endothelial cells. IHs characterized by a peculiar natural life cycle enclosing three phases: proliferative (≤12 months), involuting (≥13 and involuted (up 4–7 years). The mechanisms underlying this neoplastic disease still remain uncovered. Twenty-seven IH tissue specimens (15 12 involuting) were subjected hematoxylin eosin staining panel diagnostic markers...
Abstract The overexpression of the ecotropic viral integration site-1 gene ( EVI1/MECOM ) marks most lethal acute myeloid leukemia (AML) subgroup carrying chromosome 3q26 abnormalities. By taking advantage intersectionality high-throughput cell-based and expression screens selective pan-histone deacetylase inhibitors (HDACis) emerge as potent repressors EVI1 . To understand mechanism driving on-target anti-leukemia activity this compound class, here we dissect dynamics bone marrow cells...
Background: Intersecting genetic, biologic and clinico-pathological features with high-throughput imaging may pave the way to precision oncology. We advanced hypothesis that tumor immune microenvironment (TIME) imprint on CT scan parameters in a qualitative quantitative (radiomics) fashion, providing non-invasive approach identify new prognostic factors NSCLC patients. Methods: In this study, we enrolled sixty (31 Adenocarcinoma, 29 Squamous Cell Carcinoma) surgically resected defined TIME...
Objectives: The aim of the present study was to characterize biological properties and in vivo tumourigenic potential mesenchymal stem cells (MSC) obtained from human non-small-cell lung cancers (NSCLC).
Abstract Reticular erythematous mucinosis is a rare syndrome which can be misdiagnosed as seborrhoeic dermatitis. This long‐lasting skin disease, characterized by the histological presence of deposits mucin in dermis, usually responds to treatment with per os hydroxychloroquine, 400 mg/day, for 3 months.