A5086511878- CAR-T cell therapy research
- Effects of Radiation Exposure
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Radiation Therapy and Dosimetry
- Integrated Circuits and Semiconductor Failure Analysis
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- COVID-19 Impact on Reproduction
- Virus-based gene therapy research
- COVID-19 and healthcare impacts
- Hematopoietic Stem Cell Transplantation
- Fungal Infections and Studies
- Immune Response and Inflammation
- Biosimilars and Bioanalytical Methods
- Immunodeficiency and Autoimmune Disorders
- Cancer Immunotherapy and Biomarkers
- Radiation Dose and Imaging
- Nanowire Synthesis and Applications
- Ultrasound and Hyperthermia Applications
- Neutropenia and Cancer Infections
- Biomarkers in Disease Mechanisms
- Renal Transplantation Outcomes and Treatments
- Biomedical Ethics and Regulation
University of Maryland, Baltimore
2016-2025
U-M Rogel Cancer Center
2005-2025
University of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center
2020-2024
University of Maryland Medical System
2021
University of Maryland Medical Center
2000-2014
University of Maryland, College Park
2002
Leukocyte growth factors (LGF), such as filgrastim, pegfilgrastim and sargramostim, have been used to mitigate the hematologic symptoms of acute radiation syndrome (ARS) after accidents. Although these pharmaceuticals are currently approved for treatment chemotherapy-induced myelosuppression, approval has not granted myelosuppression resulting from exposure. Regulatory drugs treat radiological or nuclear exposure injuries requires their development testing in accordance with Animal Efficacy...
MHC class I chain-related antigen A (MICA) and B (MICB) are HLA related products of polymorphic genes. Constitutive expression in normal tissue is limited to gut epithelium but can be induced other epithelial cells by stress. Specific antibodies against MICA have been reported the serum patients who had rejected kidney allografts, suggesting a potential role for these molecules transplant immunopathology. However, MICB transplanted organs has not demonstrated. In this study, we report renal...
Solid organ transplant recipients (SOTR) receiving post-transplant immunosuppression show increased COVID-19-related mortality. It is unclear whether an additional dose of COVID-19 vaccines can overcome the reduced immune responsiveness against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants.We analysed humoral responses SARS-CoV-2 and its variants in 53 SOTR vaccination.Following initial vaccination series, 60.3% showed no measurable neutralisation only 18.9%...
The use of plasma citrulline as a biomarker for acute and prolonged gastrointestinal injury via exposure to total- partial-body irradiation (6 MV LINAC-derived photons; 0.80 Gy min) in nonhuman primate models was investigated. covered injuries spanning lethal, mid-lethal, sub-lethal doses. assessed measurement small intestinal histopathology over the first 15 d following radiation included total-body at 13.0 Gy, 10.5 7.5 11.0 with 5% bone marrow sparing. dosing schemes time points out day 60...
Polysialic acid (polySia) is a unique linear homopolymer of α2,8-linked sialic that has been studied extensively as posttranslational modification neural cell adhesion molecule in the central nervous system. Only two proteins are known to be polysialylated cells immune system: CD56 on human natural killer and murine bone marrow (BM) leukocytes, neuropilin-2 (NRP-2) dendritic (DCs). We tested hypothesis polySia expression regulated during maturation migration leukocytes plays role functional...
The development of medical countermeasures against acute and delayed multi-organ injury requires animal models predictive the human response to radiation its treatment. Late chronic is a well-known feature nephropathy, but kidney has not been reported in an appropriate model. We have established single-fraction partial-body irradiation model with minimal marrow sparing non-human primates. Subject-based management was used including parenteral fluids according prospective morbidity criteria....
A nonhuman primate model of acute, partial-body, high-dose irradiation with minimal (2.5%) bone marrow sparing was used to assess endogenous gastrointestinal and hematopoietic recovery the ability Neulasta (pegylated granulocyte colony-stimulating factor) or Neupogen (granulocyte enhance from myelosuppression when administered at an increased interval between exposure initiation treatment. secondary objective effect on mortality morbidity due acute radiation syndrome concomitant syndrome....
Abstract Idecabtagene vicleucel (ide-cel) and ciltacabtagene autoleucel (cilta-cel) are approved chimeric antigen receptor T cell (CAR T) therapies for multiple myeloma. Unfortunately, most patients receiving these treatments will experience toxicities and/or relapse highlighting the need optimizing CAR strategies. We performed first in-depth, comparative prospective biomonitoring of (N=39) cilta-cel or ide-cel in real-world setting. Cilta-cel response rates were higher, although not...
Delayed immune reconstitution remains a major cause of morbidity associated with myelosuppression induced by cytotoxic therapy or myeloablative conditioning for stem cell transplant, as well potentially lethal doses total- partial-body irradiation. Restoration functional repertoire requires hematopoietic all cells and effective thymopoiesis T recovery. There are no medical countermeasures available to mitigate damage consequent high-dose, irradiation, there characterized large animal models...
Acute and chronic kidney injury may occur after accidental prompt radiation exposures. We have modeled their occurrence in a nonhuman primate model. Subjects who are exposed to more than 5-Gy irradiation apt show blood cell cytopenias be treated with granulocyte colony-stimulating factors such as Neupogen® or Neulasta® mitigate the hematologic of acute syndrome. Neupogen Neulasta now approved by US Food Drug Administration for this indication. This will significantly increase number...
Abstract Background Aggressive B cell lymphoma with secondary central nervous system (CNS) involvement (SCNSL) carries a dismal prognosis. Chimeric antigen receptor (CAR) T cells (CAR-T) targeting CD19 have revolutionized the treatment for lymphomas; however, only single cases CNS manifestations successfully treated CAR-T been reported. Methods We prospectively enrolled 4 patients SCNSL into our study to assess clinical responses and monitor immunity. Results Two of four SNCSL responded...
Recovery from severe immunosuppression requires hematopoietic stem cell reconstitution and effective thymopoiesis to restore a functional immune repertoire. Herein, model of consequent potentially lethal doses irradiation is described, which may be valuable in evaluating potential medical countermeasures. Male rhesus macaques were total body irradiated by exposure 6.00 Gy 250 kVp x-radiation (midline tissue dose, 0.13 min), resulting an approximate LD10/60 (n = 5/59). Animals received...
Summary In the relapsed/refractory setting for treatment of large B‐cell lymphoma (LBCL), chimeric antigen receptor T‐cell (CAR‐T) therapy has emerged as an effective modality. Patients often have aggressive disease that requires prompt in form bridging (BT) stabilisation while CAR‐T cells are manufactured. ( n = 75) undergoing infusion LBCL at our institution were identified. A total 52 (69·3%) received BT and 23 (30·7%) no (NBT). modalities included systemic (SBT) 28 patients, radiation...
Cachexia, or muscle wasting, is a serious health threat to victims of radiological accidents patients receiving radiotherapy. Here, we propose non-human primate (NHP) radiation-induced cachexia model based on clinical and molecular pathology findings. NHP exposed potentially lethal partial-body irradiation developed symptoms such as body weight loss in time- dose-dependent manner. Severe high 20-25% was observed which refractory nutritional intervention. Radiographic imaging indicated that...
Key Points A severely immunocompromised patient with MM and COVID19 who received a convalescent plasma product showed SARS-CoV-2 clearance. The humoral immunity against all structural proteins, which was successfully transferred to the patient.
Background/aims Chimeric antigen receptor T-cell (CAR T) therapy has been shown to improve the remission rate and survival for patients with refractory haematological malignancies. The aim of this study is describe ocular adverse effects associated CAR T in Methods This a retrospective, single-institution, case series. Patients aged 18 years or older who received standard care relapsed/refractory large B-cell lymphoma documented ophthalmic evaluation were included. primary outcome was...
Chimeric antigen receptor T-cell (CAR-T) therapy has emerged as a rapidly-advancing treatment modality for relapsed/refractory (R/R) large B-cell lymphoma (LBCL) with response rates approaching 60–80%.1, 2 The registrational clinical trials that lead to the approval of CAR-T in R/R LBCL, well ongoing trials, have excluded patients previous allogeneic haematopoietic cell transplantation (alloHCT) from trial participation.3-8 To date, limited data exists describing safety and efficacy...
Summary Idiopathic CD4 + T lymphocytopenia (ICL) is defined as a T‐cell count <0·3 × 10 9 /l or <20% of the total on two occasions in absence any immunodeficiency disorder therapy associated with reduced count. Although several mechanisms ICL have been reported, pathophysiology still largely unknown. This case report describes patient who presented cryptococcal meningitis and was subsequently discovered to meet criteria for ICL. Flow cytometric analysis patient's peripheral blood...
Exposure to sufficiently high doses of ionizing radiation is known cause fibrosis in many different organs and tissues. Connective tissue growth factor (CTGF/CCN2), a member the CCN family matricellular proteins, plays an important role development multiple organs. The aim present study was quantify gene protein expression CTGF variety from non-human primates (NHP) that were previously exposed potentially lethal radiation. Tissues non-irradiated NHP whole thoracic lung irradiation (WTLI) or...