Ovarian cancer is the fifth leading cause of female death globally due to its low survival rates. Thus, improved approaches for ovarian detection are urgently needed. MicroRNAs as a new class biomarkers have been explored in recent studies. This study was trying identify and validate two kinds serum microRNA (miR-200c miR-141) cancer. We extracted samples from 74 epithelial patients, 19 borderline cancer, 50 healthy controls. Relative expression these miRNA markers were measured by...

Background: Increasing evidence has revealed that the aberrant expression of microRNAs (miRNAs) plays vital roles in development and progression ovarian cancer. MiR-200a-3p was found to act as an oncogene a variety cancers, however, function miR-200a-3p cancer not been characterized. Materials methods: The tissues cell lines detected by RT-qPCR. influence on growth cells determined with Cell Counting Kit-8 assay, colony formation invasion assay. binding 3ʹ-untranslated region (UTR) PDCH9...

Background Neoadjuvant, endocrine, and targeted therapies have significantly improved the prognosis of breast cancer (BC). However, due to high heterogeneity cancer, some patients cannot benefit from existing treatments. Increasing evidence suggests that amino acids their metabolites can alter tumor malignant behavior through reshaping microenvironment regulation immune cell function. Breast lines been identified as methionine-dependent, methionine restriction has proposed a potential...

Almost 90% of head and neck malignancies are malignant squamous cell cancers, making it the sixth most common malignancy in developing countries, with an overall five-year survival rate about 40%-50%. Early diagnosis treatment can bring a better prognosis. Fibroblast growth factor (FGF) is important polypeptide vivo. Studies have found that FGF signal has carcinogenic potential participates variety behaviors. Some experiments proved function tumor inhibition some cases, role signalling...

Premature ovarian failure, a reproductive dysfunction characterized by follicle loss leads to premature menopause. Apoptosis of granulosa cells may be responsible for the associated depletion. MicroRNAs are expressed abundantly in and play an important role follicular atresia. Evidence suggests that signal transducer activator transcription 3 (STAT3) is involved growth female fertility.We incubated cultured mouse (mGCs) with increasing doses cisplatin (CP) varying periods. Cell proliferation...

Oxaliplatin resistance limits the efficiency of treatment for colorectal cancer (CRC). Studies have shown that abnormal expression microRNAs (miRNAs) were associated with tumorigenesis, development and chemoresistance. The purpose this study was to identify potential miRNAs related oxaliplatin in CRC cells. In work, using small RNA sequencing (small RNA-Seq) transcriptome (RNA-Seq), we found down-regulated miR-483-3p concurrent up-regulated FAM171B oxaliplatin-resistant cell line HCT116/L as...

Abstract Objective Apurinic endonuclease 1 (APE1) has been suggested as an oncogene of lung tumours and our bioinformatics analysis identified the association between Erlotinib resistance interleukin‐6 (IL‐6). Thus, we performed this work to delineate mechanistic actions APE1/IL‐6 signalling in non‐small cell cancer (NSCLC). Methods We selected human NSCLC lines HCC827 PC9 establish Erlotinib‐resistant HCC827R PC9R cells. Cancer stem cells (CSCs) were isolated from Erlotinib‐sensitive...

A novel electrode modified with silver nanoparticles was fabricated. It is found that the reducibility of higher than for bulk by comparing a micro-disk electrode. When SCN- added, new oxidation peak occurred and anodic current decreased. The proportional to thiocyanate concentration in range 5.0×10-7~4.0×10-4 mol/L pH 6.0 NaH2PO4-Na2HPO4 buffer solutions (PBS). detection limit (S/N=3) 4×10-8 mol/L. This method has been applied determination saliva (smoker non-smoker).

Background Adenocarcinoma with positive echinoderm microtubule-associated protein-like 4 gene and anaplastic lymphoma kinase (EML4-ALK) fusion accounts for 3-7% of lung cancer cases can be targeted ALK tyrosine inhibitors (TKIs). Second-generation TKIs are the standard care populations, especially those central nervous system (CNS) metastasis. However, most patients eventually experience disease progression because drug resistance caused by multiple mechanisms, predominantly secondary...

A novel electrode modified with silver nanoparticles was fabricated. It is found that the reducibility of higher than for bulk by comparing a micro-disk electrode. When SCN- added, new oxidation peak occurred and anodic current decreased. The proportional to thiocyanate concentration in range 5.0×10-7~4.0×10-4 mol/L pH 6.0 NaH2PO4-Na2HPO4 buffer solutions (PBS). detection limit (S/N=3) 4×10-8 mol/L. This method has been applied determination saliva (smoker non-smoker).

Abstract Serous ovarian cancer is one of the most fatal gynecological tumors with an extremely low 5-year survival rate. Most patients are diagnosed at advanced stage wide metastasis. The dysregulation genes serves important role in metastasis progression cancer. Differentially expressed (DEGs) between primary and metastases serous were screened out gene expression profile GSE73168 from Gene Expression Omnibus (GEO). Cytoscape plugin cytoHubba weighted co-expression network analysis (WGCNA)...

Well-oriented 3D gold flower-like nanoparticles were successfully synthesized by a facile one-pot method, and the nanoflowers (AuNFs) mixed with hemoglobin (Hb) to form nanoflowers/hemoglobin composite. The composite was further combined multiwalled carbon nanotubes on glassy electrode (GCE) fabricate novel biosensor. sensor has high stability bioactivity, studied scanning electron microscopy (SEM) cyclic voltammetry (CV). hemoglobin/gold nanoflowers/multiwalled (Hb/AuNFs/CNTs/GCE) either...

Tumor‑associated macrophages (TAMs) promote the progression of endometrial cancer (EC), but mechanism TAM in EC cell proliferation remains unclear. It was found that colony stimulating factor (CSF)‑1 and CSF‑1 receptor (CSF‑1R) were highly expressed tissues patients two lines (ECC‑1 HEC‑1A). Using wound‑healing chemotactic migration assays to evaluate role cells induction macrophage migration, it supernatant promoted line (U937) migration; however, capacity U937 weakened when CSF‑1R blocked....

BACKGROUND:The aim of this work was to study the FABP4 and PTEN gene expression correlation in liver, muscle, adipose tissues type 2 diabetes mellitus (T2DM) rats. MATERIAL AND METHODS:Male Wistar rats (8 weeks old) were randomly divided into groups (n=12/group): a control group fed normal diet for 8 an experimental high-fat, high-sugar that received 25 mg/kg streptozotocin by intraperitoneal injection induce T2DM. The random blood glucose, fasting insulin levels measured. Pten Fabp4...

A number of studies have demonstrated that altered expression levels microRNA‑300 (miR‑300) are associated with tumor progression; however, little is understood regarding the role miR‑300 in hepatocellular carcinoma (HCC). The present study aimed to investigate expression, biological function and potential regulatory mechanism HCC. mimic inhibitor were transfected into liver cancer cells using RNAiMAX reagent. miR mRNA detected by reverse transcription‑quantitative polymerase chain reaction....