A5037659678- MicroRNA in disease regulation
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- Dialysis and Renal Disease Management
- RNA modifications and cancer
- Carcinogens and Genotoxicity Assessment
- Nanoplatforms for cancer theranostics
- Renal and related cancers
- Phytochemicals and Antioxidant Activities
- Genomics and Chromatin Dynamics
- Melanoma and MAPK Pathways
- RNA and protein synthesis mechanisms
- Metal-Organic Frameworks: Synthesis and Applications
- Bladder and Urothelial Cancer Treatments
- FOXO transcription factor regulation
- Liver Disease Diagnosis and Treatment
- Fungal Biology and Applications
- Circular RNAs in diseases
- Liver physiology and pathology
- Cancer Mechanisms and Therapy
- Cell Adhesion Molecules Research
Yale University
2005-2025
Suzhou Municipal Hospital
2024
Nanjing Medical University
2024
National Central University
2014-2023
Broad Institute
2020-2022
Shanxi Academy of Forestry
2020
The University of Texas Health Science Center at San Antonio
2003-2005
A high-throughput peptide array approach reveals insight into kinase substrates and specificity.
End joining of double-strand breaks (DSBs) requires Ku proteins and frequently involves base pairing between complementary terminal sequences.To define the role in end joining, two oppositely oriented HO endonuclease cleavage sites separated by 2.0 kb were integrated into yeast chromosome III, where constitutive expression creates simultaneous DSBs with no sequence.Lack sequence their 3 single-strand overhangs facilitates efficient repair events distinctly different from when ends have a...
Repair of chromosome double-strand breaks (DSBs) is central to cell survival and genome integrity.Nonhomologous end joining (NHEJ) the major cellular repair pathway that eliminates DSBs.Here we report our genetic screen identified Rsc8 Rsc30, subunits Saccharomyces cerevisiae chromatin remodeling complex RSC, as novel NHEJ factors.Deletion RSC30 gene or C-terminal truncation RSC8 impairs a DSB created by HO endonuclease in vivo.rsc30⌬ maintains robust level homologous recombination...
Previous studies reported that peracetylated (-)-epigallocatechin-3-gallate (AcEGCG) has antiproliferative and anti-inflammatory activities. Here, we evaluated the chemopreventive effects underlying molecular mechanisms of dietary administration AcEGCG EGCG in dextran sulfate sodium (DSS)-induced colitis mice. The mice were fed a diet supplemented with either or prior to DSS induction. Our results indicated was more effective than preventing shortening colon length formation aberrant crypt...
// Szu-Mam Liu 1 , Jean Lu 2 Hoong-Chien Lee 1,3,4 Feng-Hsiang Chung 1,3 and Nianhan Ma Institute of Systems Biology Bioinformatics, National Central University, Jhongli, Taiwan Genomics Research Center, Academia Sinica, Taipei, 3 Center for Dynamical Biomarkers Translational Medicine, 4 Department Physics, Yuan Christian Correspondence: Ma, email: Keywords : miR-524-5p, melanoma, BRAF, ERK2, MAPK signaling, microRNA Received June 20, 2014 Accepted September 08, Published Abstract It has...
Using a simple method, the aldehyde groups of zeolitic imidazolate framework-90 (ZIF-90) nanocrystals were converted into carboxyl, amino, and thiol groups, without affecting integrity framework. Notably, for first time, correlations between functionality cytotoxicity are also demonstrated via in vitro assays. The positive charged aminated-ZIF-90 presumably results either perturbation cell membrane, more efficient uptake, or both. Therefore, half-maximal effective (EC50 ) concentration has...
Human embryonic stem cells (hESCs) are functionally unique for their self-renewal ability and pluripotency, but the molecular mechanisms giving rise to these properties not fully understood. hESCs can differentiate into embryoid bodies (EBs) containing ectoderm, mesoderm, endoderm. In miR-200 family, miR-200c was especially enriched in undifferentiated significantly downregulated EBs. The knockdown of Nanog expression, upregulated GATA binding protein 4 (GATA4) induced hESC apoptosis. GATA4...
Drug repurposing has become an increasingly attractive approach to drug development owing the ever-growing cost of new discovery and frequent withdrawal successful drugs caused by side effect issues. Here, we devised Functional Module Connectivity Map (FMCM) for repurposed compounds systems treatment complex diseases, applied it colorectal adenocarcinoma. FMCM used multiple functional gene modules query (CMap). The were built around hub genes identified, through a selection...
Saccharomyces cerevisiae Rad53, the ortholog of mammalian Chk2, is an essential protein kinase in DNA damage and replication checkpoint pathways. Consecutive phosphatidyl inositol kinase-like (PIKK)-dependent PIKK-independent steps activation Rad53 are key for controlling transmitting diverse downstream responses to damage. However, these activities have not been demonstrated vitro defined systems. Here, we shown that enzymatically dephosphorylated purified autoactivates through a...
Abstract In contrast to the somatic cells, embryonic stem cells (ESCs) are characterized by its immortalization ability, pluripotency, and oncogenicity. Revealing underlying mechanism of ESC characteristics is important for application ESCs in clinical medicine. We performed systematic functional screen mouse with 4,801 shRNAs that target 929 kinases phosphatases. One hundred thirty-two candidate genes regulate both expansion cell marker expression were identified. Twenty-seven out 132...
Mouse embryonic stem cells (ES cells) can proliferate indefinitely. To identify potential signals involved in suppression of self-renewal, we previously screened a kinase/phosphatase expression library ES cells, and observed that inhibition Dual Leucine zipper-bearing Kinase (DLK) increased relative cell numbers. DLK protein was detected both the pluripotent differentiated states mouse while kinase activity upon differentiation. Overexpression displayed reductions cell/colony numbers Nanog...
AbstractRad53, the ortholog of mammalian Chk2, is a major DNA damage checkpoint effector kinase in Saccharomyces cerevisiae. Despite extensive studies on genetic requirements for Rad53 activation and its linkage downstream to responses, mechanism not completely understood. Rad53-dependent signal amplification thought be primary force that accelerates transduction processes response damage. forms oligomers upon vivo. It clear how oligomer formation affects what oligomerization. Here, we...
In view of the limited knowledge plasma biomarkers relating to cancer resistance radiotherapy, we have set up screening, training and testing stages investigate microRNAs (miRNAs) expression profile in predict between poor responsive groups after 6 months radiotherapy. Plasma was collected prior microRNA profiles were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) arrays. Candidate miRNAs validated single qRT-PCR assays from set. The classifier for...
100 nm ZIF-90 nanoparticles with low cytotoxicity are obtained by utilizing glycerol for the control of viscosity effects in water–alcohol-based system.
CCM111 is an aqueous extract of Antrodia cinnamomea (AC) that has exhibited anti-liver fibrosis functions. However, the detailed mechanisms AC action against liver have not been elucidated yet. The present research showed significantly lowered levels hepatic enzyme markers glutamate oxaloacetate transaminase (GOT) and glutamic pyruvic (GPT), prevented damage collagen deposition, downregulated TGF-β/Smad signaling in a dose-dependent manner compared with CCl4 treatment alone. markedly...
The incidence of urothelial carcinoma (UC) is higher in patients undergoing chronic dialysis than the general population. This study investigated plasma miRNA profiling as ancillary diagnosis biomarker associated with UC hemodialysis. We successfully screened out and detected expression from eight through quantitative real-time PCR array analysis identified candidate miRNAs. miRNAs were then validated using single RT-PCR assays 52 samples. classifier for detection was developed by multiple...
Pseudouridine (Ψ) is the first discovered and most prevalent posttranscriptional modification, which has been widely studied during past decades. was observed in almost all kinds of RNAs shown to have important biological functions. Currently, time-consuming high-cost procedures experimental approaches limit its uses real-life Ψ site detection. Alternatively, by taking advantage explosive growth sequencing data, computational methods may provide a more cost-effective avenue. To date,...
Antrodia cinnamomea (AC) exhibits many bioactivities, including anti-inflammatory, anti-cancer, and hepatoprotection activities. Many researchers have studied the functions of components or fractions AC, but original extractions AC not been studied. In addition, detailed relationship between immune-related signaling pathways is unclear. this study, we screened effects CCM111, which extract on seven further investigated whether CCM111 can influence inflammation. Interestingly, our results...
Abstract Cancer stem cells (CSCs), or cancer with cell-like properties, generally exhibit drug resistance and have highly potent inducing capabilities. Genome-wide expression data collected at public repositories over the last few years provide excellent material for studies that can lead to insights concerning molecular functional characteristics of CSCs. Here, we conducted genomic CSC based on fourteen PCA-screened high quality whole genome gene datasets and, as control, four non-stem-like...
BRAF inhibitors were approved for the treatment of BRAF-mutant melanoma. However, most patients acquire resistance to after several months treatment. miR-524-5p is considered as a tumor suppressor in many cancers, including In this study, we investigated biological functions melanoma with acquired inhibitor and evaluated endogenous expression biomarker The results showed that was 0.481-fold lower tissues (n = 117) than nevus 40). Overexpression significantly reduced proliferative,...