A5034778675- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Birth, Development, and Health
- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Ovarian function and disorders
- Peroxisome Proliferator-Activated Receptors
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Gestational Diabetes Research and Management
- Hormonal Regulation and Hypertension
- Antioxidant Activity and Oxidative Stress
- Estrogen and related hormone effects
- Vitamin C and Antioxidants Research
- Pregnancy and preeclampsia studies
- Lipoproteins and Cardiovascular Health
- Lipid metabolism and disorders
- Caveolin-1 and cellular processes
- Paraoxonase enzyme and polymorphisms
- Pancreatic function and diabetes
- Bariatric Surgery and Outcomes
- Folate and B Vitamins Research
- Adipokines, Inflammation, and Metabolic Diseases
- Infant Nutrition and Health
- Lipid metabolism and biosynthesis
- Lysosomal Storage Disorders Research
Experimental Medicine and Biology Institute
2001-2024
Universidad de Los Andes, Chile
2020-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2006-2024
Universidad Maimónides
2024
University of Chicago
2024
Second Affiliated Hospital of Fujian Medical University
2024
Fujian Medical University
2024
Pontificia Universidad Católica de Chile
2011-2020
Universidad de Los Andes
2020
Diabetes Australia
2014
CRISP2, originally known as Tpx-1, is a cysteine-rich secretory protein specifically expressed in male haploid germ cells. Although likely to be involved gamete interaction, evidence for functional role of CRISP2 fertilization still remains poor. In the present study, we used mouse model examine subcellular localization sperm and its involvement different stages fertilization. Results from indirect immunofluorescence extraction experiments indicated that an intraacrosomal component...
Epididymal protein CRISP1 participates in rat and mouse gamete fusion through its interaction with complementary sites on the egg surface. Based vivo observations, present study we investigated possibility that plays an additional role sperm-zona pellucida (ZP) precedes fusion. In vitro fertilization experiments using zona-intact eggs indicated presence of either antibody against (anti-CRISP1) or native (rCRISP1) during co-incubation produced a significant decrease percentage fertilized...
Objective Infants born from overweight and obese mothers with glucose-controlled gestational diabetes (GDM) tend to be large-for-gestational age (LGA). It is hypothesized that this due an excessive rise in maternal triglyceride levels. Methods Two-hundred seventy nine singleton GDM pregnancies were divided into three groups according prepregnancy BMI: normal weight (BMI = 20–24.9; n 128), 25–29.9; 105), ≥ 30; 46). Individual z-scores (ZS) of triglycerides newborn (NWZS) calculated as...
Significance Production of functional sperm and eggs requires a complex process called meiosis. Meiosis in mouse human pauses at stage metaphase II (MII) arrest until fertilization by sperm. After fertilization, released from MII complete meiosis develop into new individuals. In analyzing the female infertility genetically altered mice, we discovered that excess cholesterol can trick behaving as though they were fertilized (released arrest), thus disrupting normal synchrony between...
Human epididymal sperm protein ARP, a member of the cysteine-rich secretory proteins (CRISP) family, exhibits significant homology with rat DE, candidate molecule for mediating sperm-egg fusion in rodents. The aim this study was to investigate involvement ARP human gamete fusion. Sequential extraction from ejaculated revealed existence population that is tightly associated surface and thus, potentially capable participating interaction. Exposure capacitated polyclonal antibody against...
Testicular protein Tpx-1, also known as CRISP-2, is a cysteine-rich secretory specifically expressed in the male reproductive tract. Since information available on human limited to identification and expression of its gene, this work we have studied presence localization Tpx-1 (TPX1) sperm, fate after capacitation acrosome reaction (AR), possible involvement gamete interaction. Indirect immunofluorescence studies revealed absence significant staining live or fixed non-permeabilized contrast...
Human epididymal CRISP1 (hCRISP1) associates with sperm during maturation and participates in gamete fusion through egg complementary sites. Its homology both rodent CRISP4 reported to participate the previous stage of binding zona pellucida (ZP), led us further investigate functional role hCRISP1 by studying its involvement human sperm–ZP interaction. hemizona (HZ) were inseminated capacitated presence either anti-hCRISP1 polyclonal antibody inhibit hCRISP1, or bacterially-expressed...
A healthy dietary pattern and high quality nutrient intake reduce atherosclerotic cardiovascular disease risk. Red wine grape pomace (RWGP)—a rich natural source of fiber antioxidants—appears to be a potential functional food ingredient. The impact supplementation with RWGP flour was evaluated in atherogenic diet-fed SR-B1 KO/ApoER61h/h mice, model lethal ischemic heart disease. mice were fed (high fat, cholesterol, cholic acid, HFC) diet supplemented with: (a) 20% chow (HFC-Control), (b)...
Rat epididymal glycoprotein DE associates with the dorsal region of sperm head during maturation, migrates to equatorial segment (ES) acrosome reaction (AR), and is involved in gamete membrane fusion. In present study we examined association surface relationship this interaction behavior function protein. Cloning sequencing revealed a lack hydrophobic domains presence 16 cysteine residues molecule. Experiments which cauda were subjected different extraction procedures indicated that while...
Nicotine, the most important neuroteratogen of tobacco smoke, can reproduce brain and cognitive disturbances per se when administered prenatally. However, it is still unknown if paracrine signaling among cells participates in prenatal nicotine-induced impairment adult offspring. Paracrine partly mediated by unopposed channels formed connexins hemichannels (HCs) pannexins serving as aqueous pores permeable to ions small molecules, allowing exchange between intra- extracellular milieus. Our...
Rat sperm epididymal glycoprotein DE belongs to the cysteine-rich secretory protein (CRISP) family and participates in sperm-egg fusion through its binding complementary sites on egg surface. To investigate molecular mechanisms underlying role of gamete fusion, present work we expressed a prokaryotic system, examined relevance carbohydrates disulfide bonds for biological activity protein. Immunofluorescence assays carried out presence recombinant (recDE) revealed that this exhibits ability...
The srbi gene encodes a lipoprotein receptor with high affinity for density that is mainly expressed in the liver and steroidogenic tissues. Disruption of this mice mutations humans lead to alterations metabolism and/or fertility. During murine development, scavenger class B member I (SR-BI) present yolk sac placenta only embryo itself late gestation. In humans, it has been detected trophoblast cells placenta. Although proportion carrying null mutation SR-BI obtained from heterozygous...
Aims Pancreatic β-cells synthesize and release serotonin (5 hydroxytryptamine, 5HT); however, the role of 5HT receptors on glucose stimulated insulin secretion (GSIS) mechanisms mediating this function is not fully understood. The aims study were to determine expression profile in murine MIN6 examine effects pharmacological activation receptor Htr2b GSIS mitochondrial function. Materials Methods mRNA levels cells quantified by RT qPCR. was assessed response global serotonergic with or...
Numerous studies have demonstrated that sperm capacitation is a bicarbonate-dependent process. In the rat, has not been studied as much in other species, mainly because of difficulties carrying out functional assays with this animal model. present study, we examined influence bicarbonate overall rat process by analyzing involvement anion 1) protein tyrosine phosphorylation, 2) migration epididymal DE (also known CRISP-1) from dorsal region to equatorial segment head occurs during...
The cholesterol content of the sperm membrane is regulated during both maturation in epididymis and capacitation female tract, two processes required for spermatozoa to acquire their fertilising ability. Because Niemann-Pick disease, type C2 (NPC2) protein one most abundant components epididymal fluid contains a functional cholesterol-binding site that can transfer between membranes, it has been suggested years NPC2 could be involved regulation levels maturation. In present study, western...
SR-BI is the main receptor for high density lipoproteins (HDL) and mediates bidirectional transport of lipids, such as cholesterol vitamin E, between these particles cells. During early development, expressed in extraembryonic tissue, specifically trophoblast giant cells parietal yolk sac. We previously showed that approximately 50% SR-BI-/- embryos fail to close anterior neural tube develop exencephaly, a perinatal lethal condition. Here, we evaluated role embryonic E uptake during murine...
Atherosclerotic cardiovascular disease is highly prevalent and its underlying pathogenesis involves dyslipidemia including pro-atherogenic high density lipoprotein (HDL) remodeling. Vitamins C E have been proposed as atheroprotective agents for management. However, their effects benefits on function remodeling are unknown. In this study, we evaluated the role of vitamin non HDL lipoproteins well remodeling, along with inflammation/oxidation biomarkers atherosclerosis in atherogenic diet-fed...