A5043306047- Cancer Cells and Metastasis
- Celiac Disease Research and Management
- Microscopic Colitis
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Nanoparticles: synthesis and applications
- Graphene and Nanomaterials Applications
- Occupational and environmental lung diseases
- Nanoparticle-Based Drug Delivery
- Inflammatory Biomarkers in Disease Prognosis
- Advanced biosensing and bioanalysis techniques
- Protease and Inhibitor Mechanisms
- Prostate Cancer Treatment and Research
- Galectins and Cancer Biology
- Cancer Immunotherapy and Biomarkers
- Cancer, Lipids, and Metabolism
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
- Gold and Silver Nanoparticles Synthesis and Applications
- Helicobacter pylori-related gastroenterology studies
- Immune Cell Function and Interaction
- Cancer Treatment and Pharmacology
- Cancer, Hypoxia, and Metabolism
- Advanced Biosensing Techniques and Applications
Trinity College Dublin
2013-2024
Coombe Women & Infants University Hospital
2020-2024
St. James's Hospital
2004-2023
Wye Valley NHS Trust
2023
Gloucestershire Hospitals NHS Foundation Trust
2021
Cheltenham General Hospital
2021
Cancer Institute (WIA)
2019
North Bristol NHS Trust
2018
University of Anbar
2011
Hôpital Beaujon
2010
Advancement of biomedical applications carbonaceous nanomaterials is hampered by their biopersistence and pro-inflammatory action in vivo. Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice showed that oxidation clearance single walled carbon nanotubes (SWCNT) from the lungs these animals after pharyngeal aspiration was markedly less effective whereas inflammatory response more robust than wild-type C57Bl/6 mice. Our results provide direct evidence for participation MPO – one...
Despite the widespread availability of immunohistochemical and other methodologies for screening early detection lung breast cancer biomarkers, diagnosis stage cancers can be difficult prone to error. The identification validation biomarkers specific cancers, which would permit development more sensitive methods disease onset, is urgently needed. In this paper, ultra-small bright nanoprobes based on quantum dots (QDs) conjugated single domain anti-HER2 (human epidermal growth factor receptor...
Aim: Rapidly expanding manufacture and use of nanomaterials emphasize the requirements for thorough assessment health outcomes associated with novel applications. Post-translational protein modifications catalyzed by Ca2+-dependent peptidylargininedeiminases have been shown to trigger immune responses including autoantibody generation, a hallmark complexes deposition in rheumatoid arthritis. Therefore, aim study was assess if nanoparticles are able promote citrullination. Materials &...
Nanomaterials such as SiO2 nanoparticles (SiO2NP) are finding increasing applications in the biomedical and biotechnological fields disease diagnostics, imaging, drug delivery, food, cosmetics biosensors development. Thus, a mechanistic systematic evaluation of potential biological toxic effects SiO2NP becomes crucial order to assess their complete safe applicability limits. In this study, human monocytic leukemia cell line THP-1 alveolar epithelial A549 were exposed range amorphous various...
Amorphous silica nanoparticles (ASNP) can be synthetized via several processes, 2 of which are the thermal route (to yield pyrogenic silica) and wet from a solution containing silicate salts obtain precipitated, colloidal, mesoporous silica, or gel). Both methods synthesis lead to ASNP that applied as food additive (E551). Current regulation does not require production additives indicated on product label, and, thus, listed without mentioning method. Recent results indicate, however, more...
Abstract Circulating tumour cells (CTCs) are a critical intermediate step in the process of cancer metastasis. The reliability CTC isolation/purification has limited both potential to report on metastatic progression and development CTCs as targets for therapeutic intervention. Here we new methodology, which optimises culture conditions using primary model system. We exploited known biology that thrive hypoxic conditions, with their survival proliferation being reliant activation...
Traditional treatment of cancer has been plagued by a number obstacles, such as multiple drug resistance, toxicity and financial constraints. In contrast, phytochemicals that modulate variety molecular mechanisms are garnering increasing interest in complementary alternative medicine. Therefore, an approach based on network pharmacology was used the present study to explore possible regulatory 6-shogaol potential for cervical (CC). A public databases were screened collect information target...
Diagnosis and assessment of patients with prostate cancer is dependent on accurate interpretation grading histopathology. However, morphology does not necessarily reflect the complex biological changes occurring in disease progression, current biomarkers have demonstrated limited clinical utility patient assessment. This study aimed to develop that accurately define biology by distinguishing specific pathological features enable reliable pathology for Gleason patients. Online gene expression...
Abstract Prostate cancer (PCa) development and progression relies on the programming of glucose lipid metabolism, this involves alterations in androgen receptor expression signalling. Defining molecular mechanism that underpins metabolic will have direct significance for patients with PCa who a poor prognosis. Here we show there is dynamic balance between sortilin syndecan-1, reports different phenotypes. Using tissue microarrays, demonstrated by immunohistochemistry was highly expressed...
Platinum resistance is a major cause of treatment failure in ovarian cancer. We previously identified matrix metalloproteinase 9 (MMP-9) as potential therapeutic target chemoresistant disease. A2780cis (cisplatin-resistant) and A2780 (cisplatin-sensitive) carcinoma cell lines were used. The cytotoxic effect MMP-9/MMP-2 inhibitor, (2R)-2-[(4-Biphenylsulfonyl) amino]-3 phenylpropionic acid (C21H19NO4S) alone or combination with cisplatin was determined using high content screening. Protein...
Coeliac disease is a gluten-sensitive enteropathy that develops in genetically susceptible individuals. The exhibits many features of an autoimmune disorder. These include the production highly specific anti-endomysial autoantibodies directed against enzyme tissue transglutaminase. It well accepted wheat-, barley- and rye-based foods should be excluded gluten-free diet. Although several studies report oats ingestion safe this diet, potential toxicity remains controversial. In current study,...
Summary Semiconductor nanoparticles or quantum dots are being increasingly utilized as fluorescent probes in cell biology both live and fixed assays. Quantum possess an immense potential for use multiplexing assays that can be run on high content screening analysers. Depending the nature of biological target under investigation, experiments frequently required cells retaining intact membrane also those have been permeabilized to expose intracellular antigens. Fixation lines before after...
Single-walled carbon nanotubes (SWCNTs) have been widely explored as potential technologies for information systems and medical applications. The impact of SWCNTs on human health is prime concern, if a future in the manufacturing industry. This study proposes novel, inflammation-independent paradigm toxicity SWCNTs, identifying protein citrullination process early-stage indicator inflammatory responses macrophages (THP-1) subtle phenotypic damages lung epithelial (A549) cells following...
Citrullination, or the post-translational deimination of polypeptide-bound arginine, is involved in several pathological processes body, including autoimmunity and tumorigenesis. Recent studies have shown that nanomaterials can trigger protein citrullination, which might constitute a common pathogenic link to disease development. Here we demonstrated auto-antibody production serum nanomaterials-treated mice. Citrullination-associated phenomena PAD levels were found be elevated -treated cell...
Abstract Purpose The association between pathological complete response (pCR) in patients receiving neoadjuvant chemotherapy (NAC) for breast cancer and Circulating Tumour Cells (CTCs) is not clear. aim of this study was to assess whether CTC enumeration could be used predict NAC as measured by the Miller–Payne grading system. Methods Twenty-six were recruited, blood samples taken pre- post-NAC. CTCs isolated using ScreenCell device stained a modified Giemsa stain. enumerated 2 pathologists...
Abstract Immunotherapies, including immune checkpoint inhibitors, have limitations in their effective treatment of malignancies. The immunosuppressive environment associated with the tumor microenvironment may prevent achievement optimal outcomes for inhibitors alone, and nanotechnology-based platforms delivery immunotherapeutic agents are increasingly being investigated potential to improve efficacy blockade therapy. In this manuscript, nanoparticles were designed appropriate size surface...
Introduction: High-grade serous ovarian cancer (HGSOC) is the most prevalent and deadliest subtype of epithelial (EOC), killing over 140,000 people annually. Morbidity mortality are compounded by a lack screening methods, recurrence common. Plasminogen-activator-inhibitor 1 (PAI-1, protein product SERPIN E1) involved in hemostasis, extracellular matrix (ECM) remodeling, tumor cell migration invasion. Overexpression associated with poor prognosis EOC. Platelets significantly increase PAI-1...
Introduction: There is no consensus regarding the postoperative radiology imaging protocol after pelvic fracture surgery. Some institutes routinely scan all patients their surgery, others do not. The aim of this study was to assess value routine use computed tomography (CT) scans surgery and determine sensitivity conventional plain radiographs intraoperative fluoroscopy in detecting metalwork malposition. Patients Methods: clinical notes undergoing period between January 2010 December 2015...