A5074855610- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Liver Disease Diagnosis and Treatment
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cancer, Lipids, and Metabolism
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- Dermatology and Skin Diseases
- Psoriasis: Treatment and Pathogenesis
- Mitochondrial Function and Pathology
- Nutritional Studies and Diet
- Amino Acid Enzymes and Metabolism
- Mass Spectrometry Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Advanced MRI Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Metabolism, Diabetes, and Cancer
- Urticaria and Related Conditions
- Wound Healing and Treatments
- Diet and metabolism studies
- Immune Cell Function and Interaction
- Sirtuins and Resveratrol in Medicine
- PI3K/AKT/mTOR signaling in cancer
Unité de Nutrition Humaine
2013-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2013-2025
Centre de Recherche en Nutrition Humaine d'Auvergne
2010-2024
Clermont Université
2007-2021
Université Clermont Auvergne
2010-2019
Inserm
1993-2018
Centre Jean Perrin
2002-2018
Université Paris Cité
2018
Centre de Recherche Épidémiologie et Statistique
2018
Centre National de la Recherche Scientifique
2018
More than 50% of patients with aggressive Blymphomas and the majority low grade lymphomas are not cured by current therapeutic strategies. The express B cell antigen CD20 on surface this serves as target for antibody-directed therapies. Clinical studies encouraging results have been underway use achimeric anti-CD20 antibody (IDEC-C2B8), consisting human IgGl-6 constant regions variable from murine monoclonal IDEC-2B8. This study investigated potential anti-tumor rapeutic value combination...
Human epidermal cells from a small skin specimen can be grown in culture into multilayered sheets suitable for the permanent coverage of large burn wounds when used as autografts. We report hare on long-term survival such cultured allografts (EAG) across major hisocomaptibility barrier three nonimmunosuppressed adult patients, suffering chronic grafted leg ulcers, where EAG have been placed to cover conventional split-thicknes autograt donor site. The absence rejection was based upon...
Combination of metabolomics and epidemiological approaches opens new perspectives for ground-breaking discoveries. The aim the present study was to investigate first time whether plasma untargeted metabolomic profiles, established from a simple blood draw healthy women, could contribute predict risk developing breast cancer within following decade better understand aetiology this complex disease. A prospective nested case–control set up in Supplémentation en Vitamines et Minéraux...
Abstract Metabolomics of tumors may allow discovery tumor biomarkers and metabolic therapeutic targets. by two-dimensional proton high-resolution magic angle spinning nuclear magnetic resonance spectroscopy was applied to investigate metabolite disorders following treatment chloroethylnitrosourea murine B16 melanoma (n = 33) 3LL pulmonary carcinoma 31) in vivo. Treated both types resumed growth after a delay. Nitrosoureas provoke DNA damage but the consequences genotoxic stress are little...
Human keratinocytes from small skin specimens were grown on mouse 3T3 cell feeder layers into epidermal sheets free Langerhans cells and MHC class II antigen. These found to be suitable for the permanent coverage of wounds when used as autografts or allografts. We report here ultrastructural differentiation this cultured epidermis after grafting onto autologous allogeneic recipients. The was a thin but multilayered Malpighian epithelium composed at different stages differentiation....
ABSTRACT Background and Aims Metabolic dysfunction‐associated steatotic liver disease (MASLD) is the most common cause of hepatocellular carcinoma (HCC). In this study, we combine metabolomic gene expression analysis to compare HCC tissues with non‐tumoural (NTT). Methods A non‐targeted strategy LC–MS was applied 52 pairs human MASLD‐HCC NTT separated into 2 groups according fibrosis severity F0F1‐F2 versus F3F4. The genes related de Novo lipogenesis (DNL) fatty acid oxidation (FAO) has been...
Breast cancer is a major cause of death in occidental women. The role metabolism breast etiology remains unclear. Metabolomics may help to elucidate novel biological pathways and identify new biomarkers predict long before symptoms appear. aim this study was investigate whether untargeted metabolomic signatures from blood draws healthy women could contribute better understand the long-term risk developing cancer.A nested case-control conducted within SU.VI.MAX prospective cohort (13 years...
Protein phosphatase 2A (PP2A), an Akt pathway inhibitor, is considered to be activated by methylation of its catalytic subunit. Also PP2A downregulation was proposed take part in carcinogenesis. Recently, activation shown response DNA damage. To obtain further information on the role tumors and damage, we investigated relationship between activity, cell proliferation, activation, c-Myc expression PTEN activity B16 melanoma cells untreated after chloroethylnitrosourea (CENU) treatment. In...
Abstract In animal models, methionine (MET) restriction in association with chloroethylnitrosoureas led to a substantial improvement. On this basis, we initiated Phase I clinical trial of dietary MET chloroethylnitrosourea (cystemustine) treatment for patients recurrent glioma or metastatic melanoma. Our purpose was 1) determine the optimal MET-free diet duration maximum depletion plasma and 2) evaluate feasibility association. A total 10 received 4 cycles 2 wk an 1, 2, 3, consecutive days...
Abstract NMR spectroscopy‐based metabolomics still needs development in quantification procedures. A method was designed for quantitative two‐dimensional high resolution magic angle spinning (HRMAS) proton‐NMR metabolite profiling of intact cells. It uses referencing metabolite‐related signals to protein‐related and yields straightforward automatable profiling. The enables exploitation only two‐dimensionally visible metabolites combination one‐ spectra, thus providing an appreciable number...
Abstract Recent NMR spectroscopy developments, such as high‐resolution magic angle spinning (HRMAS) probes and correlation‐enhanced 2D sequences, now allow improved investigations of phospholipid (Plp) metabolism. Using these modalities we previously demonstrated that a mouse‐bearing melanoma tumor responded to chloroethyl nitrosourea (CENU) treatment in vivo by altering its Plp The aims the present study were investigate whether HRMAS proton total correlation (TOCSY) could be used...
Non-Alcoholic Fatty Liver Disease (NAFLD) is considered as the forthcoming predominant cause for hepatocellular carcinoma (HCC). NAFLD-HCC may rise in non-cirrhotic livers 40 to 50% of patients. The aim this study was identify different metabolic pathways HCC according fibrosis level (F0F1 vs. F3F4). A non-targeted metabolomics strategy applied. We analyzed 52 pairs human and adjacent non-tumoral tissues which included 26 developed severe or cirrhosis (F3F4) no mild (F0F1). Tissue extracts...
There is a rising incidence of non-alcoholic fatty liver disease (NAFLD) as well the frequency Hepato-Cellular Carcinoma (HCC) associated with NAFLD. To seek for putative metabolic pathways specific NAFLD etiology, we performed comparative metabolomics between HCC and cirrhosis. The study included 28 pairs tissue versus distant Non-Tumoral Tissue (NTT) collected from patients undergoing hepatectomy. was cirrhosis (n = 9), normal 6) 13). Metabolomics using 1H-NMR Spectroscopy on extracts...
Diet has been recognized as a modifiable risk factor for breast cancer. Highlighting predictive diet-related biomarkers would be of great public health relevance to identify at-risk subjects. The aim this exploratory study was select metabolites discriminating women at higher cancer using untargeted metabolomics.
Background: The prevention and early screening of PCa is highly dependent on the identification new biomarkers. In this study, we investigated whether plasma metabolic profiles from healthy males provide novel biomarkers associated with future risk PCa. Methods: Using Supplémentation en Vitamines et Minéraux Antioxydants (SU.VI.MAX) cohort, identified samples collected 146 cases up to 13 years prior diagnosis 272 matched controls. Plasma were characterized using ultra-high-performance liquid...
In a previous study, it was reported that secondary untreated melanoma tumors implanted several weeks after and at distance from primary chloroethylnitrosourea (CENU)-treated underwent differentiation growth inhibition. To see whether the treated tumor released soluble factors mediated response, serum transfer experiments were performed in vivo. Administration of CENU-treated tumor-bearing donors arrested proliferation, decreased vessel formation induced metabolite alterations encompassing...
Abstract Cancer cells mainly rely on glycolysis for energetic needs, and mitochondrial ATP production is almost inactive. However, cancer require the integrity of functions their survival, such as maintenance internal membrane potential gradient (ΔΨm). It thus may be predicted that ΔΨm regeneration should depend cellular capability to produce sufficient by upregulating or recruiting oxidative phosphorylation (OXPHOS). To investigate this hypothesis, we compared response an anticancer agent...