Caffeic acid phenethyl ester, an active component of propolis, has been implicated in the regulation cell growth and apoptosis, although exact mechanism this activity not elucidated. In study, we explored effects caffeic ester on growth, cycle, apoptosis β-catenin/T-cell factor signaling human colon cancer cells. Using two sporadic lines (HCT116 SW480), assayed for inhibition, cycle induction. We also β-catenin downstream target genes (cyclin D1 c-myc) mRNA protein expression by reverse...

Cancer stem cells (CSCs) can invade and metastasize by epithelial-to-mesenchymal transition (EMT). However, how they escape immune surveillance is unclear. B7H1 crucial negative co-stimulatory molecule but little information about whether it works in CSCs. Therefore, we determined the expression of EMT-associated markers colorectal cancer stem-like to investigate a possible immunoevasion way We enriched CD133+ which manifested CSCs-like properties such as higher levels other cell Oct-4...

Urban soil samples were collected from the major heavy industrial city of Baotou in Inner Mongolia, China, to investigate concentration, spatial distribution, and pollution levels metals. The study employed geoaccumulation index (Igeo), ecological risk index, interpolation methods comprehensively assess urban pollution. results indicated that apart arsenic (As) nickel (Ni), concentrations metals such as cadmium (Cd), chromium (Cr), manganese (Mn), mercury (Hg), copper (Cu), zinc (Zn), lead...

Patients with cancer frequently develop autoantibodies. The identification of tumor autoantigens may have utility in early diagnosis and immunotherapy. In this study, we used serological proteomics analysis (SERPA) to identify proteins that elicit humoral response colorectal (CRC). CRC cell line HCT116 was as a source for 2-DE subsequent Western blot which individual serum from patients analyzed An autoantibody against HSP60 identified by MS detected 13 out 25 1 15 healthy subjects....

Many factors, including duration and intensity of the unfolded protein response (UPR), dictate whether cells will adapt to endoplasmic reticulum stress or undergo apoptosis. In tuberous sclerosis (TSC), elevation mammalian target rapamycin complex 1 (mTORC1) activity has been proposed compound induction UPR transcription factors ATF4 CHOP, suggesting that could be targeted eradicate TSC1/2-null during patient therapy. Here we report control c-MYC translation by mTORC1 plays a key role in...

To study the anti-tumor effect of caffeic acid phenethyl ester (CAPE) and influence CAPE on beta-catenin associated signaling pathway in SW480 colorectal cancer (CRC) cells.SW480 cells were treated with at serial concentrations. The proliferative status was measured by methabenzthiazuron (MTT) assay. Cell cycle cell apoptosis analyzed using flow cytometry (FCM). Western blotting assay used to evaluate protein level beta-catenin, c-myc cyclinD1. Beta-catenin localization determined indirect...

Malignant melanoma predominantly occurs in the skin and mucous membranes, thus, malignant of breast is particularly rare. In current study, a case 26-year-old female with presented. On diagnosis patient, extensive metastasis had occurred. The patient refused any treatment succumbed two months after initial diagnosis. prognosis for patients this rare tumour somewhat poor. Early diagnosis, correct surgical resection comprehensive adjuvant therapy are key procedures that may improve survival...

Emerging evidence has suggested that dysregulation of miR-874-3p may be involved in tumor development and progression various types cancers. However, its expression biological function esophageal squamous cell carcinoma (ESCC) remains unclear. The aim this study was to explore the roles ESCC tumorigenesis development.Quantitative Real Time-PCR used detect related mRNAs miRNA both tissues cells. Then, statistical analysis performed determine associations with clinical features prognosis ESCC....

DNA double-strand breaks (DSB) are a major form of damage and key mechanism through which radiotherapy some chemotherapeutic agents kill cancer cells. Despite its importance, measuring DSBs is still tedious task that normally carried out by gel electrophoresis or immunofluorescence staining. Here, we report novel approach to image quantify in live mammalian cells bifragment luciferase reconstitution. N- C-terminal fragments firefly genes were fused with H2AX MDC1 genes, respectively. Our...

AIM:To investigate the molecular mechanisms of anti-cancer activity caffeic acid phenethyl ester (CAPE). METHODS:Protein profiles human colorectal cancer SW480 cells treated with or without CAPE were analysed using a two-dimensional (2D) electrophoresis gelbased proteomics approach.After electrophoresis, gels stained Coomassie brilliant blue R-250.Digital images taken GS-800 Calibrated Densitometer, and image analysis was performed PDQuest 2-D Analysis software.The altered proteins following...

Previous studies indicated that the RECQL5 gene polymorphism was associated with human cancers. However, association of breast cancer remains unclear. In present study, we investigated between polymorphisms RECQL and in a Chinese population. We selected four (rs820186, rs820196, rs820200, rs4789223) for study. The genotyping performed using TaqMan method 510 patients age- sex-matched non-cancer controls. found rs820196 rs828200 were cancer. For CC genotype (16.7 vs 9.4 %, P < 0.001) C allele...

Herein, we report our work exploring the essential requirements for fluorophore selection during development of various fluorescence applications. We assembled a library chromone-derived fluorophores with diverse structure–fluorescence properties, which allowed us to choose pairs similar structures but differing properties and compared performance selected in three types commonly used found that standard suitable is variable depending on application. (1) In imaging, strong constant under...

Interstitial cells of Cajal (ICC) are distributed throughout the gastrointestinal (GI) tract and have important functions in control GI motility. Loss ICC is associated with several motility disorders; yet, mechanisms modulating survival proliferation not fully understood. Hydrogen sulfide (H2S) has been reported to be a gaseous transmitter that regulates cellular proliferation. This study aims establish whether H2S participates regulation The effect was studied primary cultures ICC,...

Circular RNAs (circRNAs) exert a significant regulatory function on tumor progression. This work intends to probe into the biological and mechanism of circRNA_0103552 (circ_0103552) in breast cancer carcinogenesis. In this study, circ_0103552, microRNA-515-5p (miR-515-5p), cysteine-rich angiogenic inducer 61 (CYR61) mRNA expressions cells tissues were determined by quantitative real-time polymerase chain reaction, followed cell counting kit 8 Transwell experiments examine multiplication,...

The receptor tyrosine kinase EPHB2 has recently been identified as a TCF4 transcriptional target that controls the intestinal epithelial architecture through repulsive interactions with Ephrin-B ligands. Many reports have demonstrated most human colorectal cancers lose expression despite constitutive Wnt activation. Therefore, we investigated mechanisms cause down-regulation in cancer. In this study, demonstrate DNA hypermethylation was not responsible for frequent loss of Cloning and...

The 5-bromo-2'-deoxyuridine (BrdU) incorporation cell proliferation assay is the most commonly used method for assessing DNA replication. current detection of BrdU in cells relies on antibody immunostaining, but has various limitations including low specificity and poor tissue penetration. In this study, we utilised a Suzuki-Miyaura reaction to develop chemical label cellular with fluorescent boronic acid probes. coupling conditions were optimised complex environments, key observation was...

Chromenone-derived natural products include chromones (flavone, isoflavone) and coumarins. Chromenone compounds not only exhibit impressive biological activities, but also are an important resource of experimentally used fluorophores, such as, 7-amino-4-methylcoumarin (AMC). Various chromenone have reported to weak fluorescence, this has the potential interfere with measurements during AMC fluorogenic assays result in non-robust assay readouts. Several flavones isoflavones were found as...