A5083946726- Acute Myeloid Leukemia Research
- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Hematopoietic Stem Cell Transplantation
- Lymphoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Ethics in Clinical Research
- Acute Lymphoblastic Leukemia research
- Viral-associated cancers and disorders
- Immune Cell Function and Interaction
- Folate and B Vitamins Research
- Child Nutrition and Water Access
- Chronic Lymphocytic Leukemia Research
- Blood disorders and treatments
- Chronic Myeloid Leukemia Treatments
- Biomedical Ethics and Regulation
- Immunodeficiency and Autoimmune Disorders
- Pluripotent Stem Cells Research
- Polyomavirus and related diseases
- Genomic variations and chromosomal abnormalities
- Reproductive Health and Technologies
- Hematological disorders and diagnostics
- CAR-T cell therapy research
- Ethics and Legal Issues in Pediatric Healthcare
- Urticaria and Related Conditions
British Columbia Children's Hospital
2016-2025
University of British Columbia
2016-2025
Children's & Women's Health Centre of British Columbia
2011-2023
Child and Family Research Institute
2014-2016
Helen Keller International
2014
BC Cancer Agency
2003-2011
Canada's Michael Smith Genome Sciences Centre
2011
Vancouver General Hospital
2007-2010
St. Paul's Hospital
2005
Hôpital Edouard Herriot
2004
Human embryonic stem cells (hESCs) can be maintained in vitro as immortal pluripotent but remain responsive to many differentiation-inducing signals. Investigation of the initial critical events involved differentiation induction would greatly facilitated if a specific, robust, and quantitative assay for hESCs with self-renewal potential were available. Here we describe results series experiments determine whether formation adherent alkaline phosphatase-positive (AP(+)) colonies under...
Pediatric acute myeloid leukemia (pAML) is characterized by heterogeneous cellular composition, driver alterations and prognosis. Characterization of this heterogeneity how it affects treatment response remains understudied in pediatric patients. We used single-cell RNA sequencing ATAC to profile 28 patients representing different pAML subtypes at diagnosis, remission relapse. At composition differed between genetic subgroups. Upon relapse, hierarchies transitioned toward a more primitive...
Glycogen storage disease (GSD) type 1 is a rare autosomal recessive inherited condition. The 1b subtype comprises the minority of cases, with an estimated prevalence in 500,000 children. Patients glycogen are often treated granulocyte colony stimulating factor (G-CSF) for prolonged periods to improve symptoms inflammatory bowel (IBD) and face severe neutropenia decrease risk infection. Long-term G-CSF treatment may result increased myelodysplastic syndrome (MDS) or acute myeloid leukemia...
Traumatic brain injury (TBI) is a major public health concern worldwide, contributing to high rates of injury-related death and disability. Severe traumatic (sTBI), although it accounts for only 10% all TBI cases, results in mortality rate 30–40% significant burden disability those that survive. This study explored the potential metabolomics diagnosis sTBI examine probable primary secondary sTBI. Serum samples from 59 adult patients with 35 age- sex-matched orthopedic controls were subjected...
Pathology and Laboratory Medicine discipline occupies a special place in the medical school healthcare ecosystem as it bridges basic science clinical practice. In era of rapid knowledge technology evolution, new ways communication, training requirements, demand personalized precision diagnostics, this specialty is facing unprecedented challenges opportunities. Some these are institution-specific, while many shared worldwide at different magnitudes. This review shares our team efforts...
There is a need for noninvasive immunological biomarkers that can identify stable kidney allograft immune quiescence to inform individualized immunosuppression. We conducted cross-sectional, pilot cohort study evaluating the relative abundance of regulatory T cells (Tregs) effector T-cell (Teff) populations as surrogate marker long-term graft tolerance. obtained fresh peripheral blood mononuclear cell samples from pediatric transplant recipients, most with recent surveillance biopsies...
Improving patient recruitment and consent to participate in clinical studies is an important issue. The process of involves three steps: referral for contact, the preliminary interview determine interest, informed discussion. We hypothesized that putting first step into a 'Permission Contact' (PTC) platform would improve engagement, efficiency other steps process, be acceptable diverse groups. Methods: To test this hypothesis, four PTC platforms were established types outpatient health...
Iron is a natural element found in food, water and soil essential for human health. Our aim was to determine the levels of iron 25 other metals trace elements groundwater from 22 households Prey Veng, Cambodia. Water analyses were conducted using inductively coupled plasma–mass spectrometry optical emission spectrometry. Compared 2011 World Health Organization guidelines drinking quality, aluminum, manganese exceeded maximum (in 4.5, 72.7 40.9% samples, respectively). 2004 Cambodian quality...
Primary atopic disorders are a group of inborn errors immunity that skew the immune system toward severe allergic disease. Defining biology underlying these extreme monogenic phenotypes reveals shared mechanisms common polygenic disease and identifies potential drug targets. Germline gain-of-function (GOF) variants in JAK1 cause atopy eosinophilia. Modeling JAK1GOF (p.A634D) variant both zebrafish human induced pluripotent stem cells (iPSCs) revealed enhanced myelopoiesis. RNA-Seq whole...
The increased use of human biological material for cell-based research and clinical interventions poses risks to the privacy patients donors, including possibility re-identification individuals from anonymized cell lines associated genetic data. These will increase as technologies databases used become affordable more sophisticated. Policies that require ongoing linkage donors' information regulatory purposes, existing practices limit participants' ability control what is done with their...
Global standardization of ferritin assays is lacking, which could have direct implications on the accurate measurement and comparability concentration iron deficiency (ID) prevalence rates in at-risk populations.We measured serum concentrations using four immunoassays: s-ELISA AxSYM™ analyzer were compared among 420 non-pregnant Cambodian women; Centaur® XP analyzer, s-ELISA, a subset 100 Elecsys® 2010 226 Congolese children aged 6-59 months.Median (adjusted for inflammation) ranged between...
B-lymphoblastic leukemia (B-ALL) is the most common childhood malignancy, and its diagnosis requires immunophenotypically demonstrating blast B cell lineage differentiation. Expression of myeloperoxidase (MPO) in B-ALL well-described it has been recognized that a mixed phenotype acute should be made cautiously if MPO expression sole myeloid feature these cases. We sought to determine whether pediatric was associated with differences laboratory, immunophenotypic, or clinical features.We...