A5101415989- CRISPR and Genetic Engineering
- Genetic Mapping and Diversity in Plants and Animals
- GABA and Rice Research
- Rice Cultivation and Yield Improvement
- Environmental Impact and Sustainability
- Plant Stress Responses and Tolerance
- Energy, Environment, Economic Growth
- Photosynthetic Processes and Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Plant Molecular Biology Research
- Healthcare and Environmental Waste Management
- Genomics and Phylogenetic Studies
- Municipal Solid Waste Management
- Plant Virus Research Studies
- Biofuel production and bioconversion
- Rabbits: Nutrition, Reproduction, Health
- Plant Reproductive Biology
- Water resources management and optimization
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Recycling and Waste Management Techniques
- Autophagy in Disease and Therapy
- RNA Research and Splicing
- Sustainable Industrial Ecology
- Plant tissue culture and regeneration
Sichuan University
2022-2025
Jiangsu Academy of Agricultural Sciences
2013-2024
Yangzhou University
2016-2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2015-2024
Chongqing Medical University
2015-2024
Jiangsu University
2019-2023
Ministry of Agriculture and Rural Affairs
2023
Nantong University
2022
Tianjin Medical University
2022
First Affiliated Hospital of Chengdu Medical College
2022
The genetic diversity and phenotypic variability of crop agronomic traits is valued by breeders for their benefits in breeding but are limited most target traits. Genome editing has proved to be a powerful tool quick efficient creation continuous beneficial variation (Eshed Lippman, 2019). rice Waxy (Wx) gene (LOC_Os06g04200) encodes granule-bound starch synthase I (GBSSI), which determines the amylose content (AC) endosperm controlling synthesis. This one major contributors eating cooking...
Seed-setting rate is a critical determinant of grain yield in rice (Oryza sativa L.). Rapid and healthy pollen tube growth the style required for high seed-setting rate. The molecular mechanisms governing this process remain largely unknown. In study, we isolate dominant low mutant, sss1-D. Cellular examination results show that blocked about half mutant styles. Molecular cloning functional assays reveals SSS1-D encodes OsCNGC13, member cyclic nucleotide-gated channel family. OsCNGC13...
Weeds and weedy rice plague commercial fields in many countries. Developing herbicide-tolerance is the most efficient strategy to control weed proliferation. CRISPR/Cas9-mediated gene editing, which generates small InDels nucleotide substitutions at around target sites using error-prone non-homologous end joining DNA repairing, has been widely adopted for generation of novel crop germplasm with a wide range genetic variation important agronomic traits. We created allele by targeting...
Over 3 billion people live in rural, unincorporated areas globally, which are vital for habitation and production. The perceived safety of these landscapes significantly impacts health well-being. However, rural areas, as natural environments urban populations to connect with nature, have not been sufficiently addressed terms concerns. Negative factors often outweigh those promoting safety, limiting the restorative potential landscapes. This study collected audio–visual samples through...
<title>Abstract</title> Plants utilize calcium as a signaling molecule to regulate innate immunity, including PAMP-triggered immunity (PTI) and effector-triggered (ETI), in addition controlling growth development<sup>1,2</sup>. Recent extensive research has highlighted that the activation of ion (Ca<sup>2+</sup>) channels during PTI formation Ca<sup>2+</sup> ETI are crucial for plant immunity<sup>3</sup>. However, comprehension on how crops substantially augment through enhancement channel...
Abstract Soft rice with low amylose content ( AC ) ranging by 5–15% is a unique type special eating and appearance quality has become popular in the market. We resequenced W x‐mp , key allele from M ilky P rincess, J apanese variety, found that +473 mutation exon 4 both Wx‐mp its ancestor allele, Wx‐mq Q ueen. Based on this functional mutation, an allele‐specific PCR AS ‐ marker was developed proven breeding population derived cross between Chinese late variety N K eng 46 / early line ing...
A novel QTL cluster for appearance quality on Chr07 was identified using reciprocal introgression populations in different locations China. Two secondary F 2 validated with significant effect quality. Appearance (AQ) is the main determinants of market value rice. Identification affecting AQ prerequisite efficient improvement through marker-assisted selection (MAS). sets lines derived from indica Minghui 63 and japonica 02428 were used to dissect stability five traits, including grain length,...
The waxy gene (Wx) in rice, which encodes the granule bound starch synthase enzyme, is responsible for amylose synthesis. Glutinous (sticky) rice has little or no that can be used various applications, such as brewing. In this study, knockout of Wx with CRISPR/Cas9 technology was conducted two elite japonica lines, Huaidao 5 (HD5) and Suken 118 (SK118), aiming to develop sticky varieties. We achieved six homozygous T0 plants more than 200 bp deletion gene, well 36 wx-HD5 18 wx-SK118...
The GRAS gene family is one of the most important plant-specific families, which encodes transcriptional regulators and plays an essential role in plant development physiological processes. has been well characterized many higher plants such as Arabidopsis, rice, Chinese cabbage, tomato tobacco. In this study, we identified 38 genes sacred lotus (Nelumbo nucifera), analyzed their physical chemical characteristics performed phylogenetic analysis using from eight representative species to show...
The purpose of this study was to investigate the effect knockdown yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) on migration invasion rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) preliminarily elucidate mechanisms between YAP/TAZ autophagy in RA-FLS. RA-FLS stable YAP or TAZ successfully established by using lentiviral-mediated gene techniques. Wound healing assay Transwell were used evaluate Reverse transcription quantitative...
Background Isatis indigotica, the source of traditional Chinese medicine Radix isatidis (Ban-Lan-Gen), is an extremely important economical crop in China. To facilitate biological, biochemical and molecular research on medicinal chemicals I. here we report first indigotica transcriptome generated by RNA sequencing (RNA-seq). Results RNA-seq library was created using extracted from a mixed sample including leaf root. A total 33,238 unigenes were assembled more than 28 million high quality...
Rice black-streaked dwarf virus (RBSDV) belongs to the genus Fijivirus in family of Reoviridae and causes severe yield loss rice-producing areas Asia. RNA silencing, as a natural defence mechanism against plant viruses, has been successfully exploited for engineering resistance plants, including rice. In this study, we generated transgenic rice lines harbouring hairpin (hpRNA) construct targeting four RBSDV genes, S1, S2, S6 S10, encoding RNA-dependent polymerase, putative core protein,...
Dense vesicles (DVs) are Golgi-derived plant-specific carriers that mediate post-Golgi transport of seed storage proteins in angiosperms. How this process is regulated remains elusive. Here, we report a rice (Oryza sativa) mutant, named glutelin precursor accumulation8 (gpa8) abnormally accumulates 57-kDa proglutelins the mature endosperm. Cytological analyses gpa8 mutant revealed proglutelin-containing DVs were mistargeted to apoplast forming electron-dense aggregates and paramural bodies...
Platelet‑derived extracellular vesicles (PEVs), which are generated from the plasma membrane during platelet activation, may be involved in inflammatory processes of rheumatoid arthritis (RA). The motility RA fibroblast‑like synoviocytes (RA‑FLS) plays a key role development synovial inflammation and joint erosion. However, effects PEVs on RA‑FLS remain unclear. Thus, present study aimed to investigate active contents potential molecular mechanisms underlying regulating migration invasion...
Chronic kidney disease (CKD) and phenylketonuria (PKU) patients need to eat rice with low glutelin content. Therefore, breeding content varieties high yield delicious taste is one of the major goals breeders due demand for product. In this study, we designed three sgRNAs targeting nine genes generated T-DNA-free homozygous editing lines reduced compared wild-type simultaneous mutation(s) in 5-7 genes. The two even significantly lower than that cultivar, LGC-1. Compared wild-type, these...