A5003566796- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Parasitic Infections and Diagnostics
- Insect symbiosis and bacterial influences
- Synthesis and Biological Evaluation
- Parasitic Diseases Research and Treatment
- Mosquito-borne diseases and control
- Biochemical and Molecular Research
- Parasites and Host Interactions
Administración Nacional de Laboratorios e Institutos de Salud
2009-2022
Institute of Parasitology
2021
Ministerio de Salud
2017-2018
Population Health Research Institute
2017
McMaster University
2017
Chagas disease is one of the main public health issues in Latin America. Increasingly during past few decades, Trypanosoma cruzi infection has been detected North America, Europe, and Western Pacific, mainly as a result population movement. The limited availability rapid serological diagnostic tests hinders diagnosis early treatment areas endemicity nonendemicity. In collaboration with 11 national reference laboratories (NRLs) from different geographical areas, we evaluated performances...
According to the Chagas congenital transmission guides, diagnosis of infants, born Trypanosoma cruzi infected mothers, relies on detection parasites by INP micromethod, and/or persistence T. specific antibody titers at 10-12 months age.Parasitemia levels were quantified PCR in cruzi-infected children, grouped according results one-year follow-up diagnosis: A) Neonates that diagnosed first month after delivery microscopic blood examination (INP micromethod) (n = 19) had a median parasitemia...
Background. In contrast to adults, Trypanosoma cruzi-infected children have more broadly functional cruzi-specific T cells, and the total T-cell compartment exhibits fewer signs of immune exhaustion. However, not much is known about link between immunocompetence treatment efficacy for human Chagas disease. Methods. Using cytokine enzyme-linked immunosorbent spot (ELISPOT) polychromatic flow cytometry, cytometric bead assay, multiplex serological assays quantitative PCR, we evaluated T....
BackgroundCurrent algorithm for Congenital Chagas Disease (cCD) diagnosis is unsatisfactory due to low sensitivity of the parasitological methods. Moreover, loss follow-up precludes final serodiagnosis after nine months life in many cases. A duplex TaqMan qPCR kit Trypanosoma cruzi DNA amplification was prospectively evaluated umbilical cord (UCB) and peripheral venous blood (PVB) infants born CD mothers at endemic non-endemic sites Argentina.MethodsWe enrolled followed-up 370 infants;...
Infection by Trypanosoma cruzi (Chagas disease [ChD]) affects around 7 million people in the Americas, most of whom are unaware their status due to lack clinical manifestations and poor access diagnosis. Rapid diagnostic tests (RDTs) widely used for screening different infections (HIV, hepatitis B, syphilis), application ChD would facilitate diagnosis, especially remote areas where health services have scarce resources. We conducted a prospective intervention study 2018 evaluate field two...
Trypanosoma cruzi, the causing agent of Chagas disease, can be transmitted to offspring infected pregnant women, thus being an epidemiologically important way parasite transmission in humans. In addition, migration women from endemic areas nonendemic countries may export this infection. The diagnosis congenital disease relies on detection because maternal antibodies are passively transferred infants during pregnancy. infection also confirmed by infant-specific anti–T cruzi at 10 months after...
trans-Sialidase and cruzipain are important virulence factors from Trypanosoma cruzi, the etiological agent of Chagas disease, that have highly antigenic domains in their structure were reported as potential tools for diagnosis illness. The aim present study is to assess possibility using catalytic domain trans-sialidase a Surface Plasmon Resonance-based immunosensor chronic disease. Immunoassays carried out with canine sera verified allows detection anti-Trypanosoma cruzi antibodies whereas...
A C T Chagas disease (CD) is one of the main neglected tropical diseases, diagnosed mainly by serological tests performed in centralized laboratories, which severely limits clinical management communities with scarce resources.Herein, an electrochemical impedance biosensor for detection CD was developed first time using a cruzipain-based sensor surface.The protein, highly immunogenic and isolated from Trypanosoma cruzi, immobilized over surface gold disc electrodes modified...
Chagas disease is a chronic parasitological disease, which could cause cardiac manifestations in approximately one-third of affected individuals. Benznidazole and nifurtimox are used to treat this infection caused by Trypanosoma cruzi. Conventionally, the criterion for cure consistently negative serological tests after treatment. We report case patient who was treated when she 13 years old achieved T. cruzi seroconversion but developed cardiomyopathy as an adult.