A5008734896- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Galectins and Cancer Biology
- Parasites and Host Interactions
- Parasitic Infections and Diagnostics
- Multiple Sclerosis Research Studies
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Signaling Pathways in Disease
- Macrophage Migration Inhibitory Factor
Experimental Medicine and Biology Institute
2015-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2022
Academia Nacional de Medicina
2022
Instituto de Biomedicina y Genética Molecular de Valladolid
2021
This study aimed to assess analytical parameters of a prototype LAMP kit that was designed for detection Trypanosoma cruzi DNA in human blood. The is based on the amplification highly repetitive satellite sequence T.cruzi microtubes containing dried reagents inside caps. reaction carried out at 65°C during 40 minutes. Calcein allows direct amplified products with naked eye. Inclusivity and selectivity were tested purified from stocks belonging six discrete typing units (DTUs), other...
Background Chronic Chagas cardiomyopathy caused by Trypanosoma cruzi is the result of a pathologic process starting during acute phase parasite infection. Among different factors, specific recognition glycan structures glycan-binding proteins from or mammalian host cells may play critical role in evolution Methodology and Principal Findings Here we investigated contribution galectin–1 (Gal–1), an endogenous protein abundantly expressed human mouse heart, to pathophysiology T. infection,...
A Trypanosoma cruzi Loopamp kit was recently developed as a ready-to-use diagnostic method requiring minimal laboratory facilities. We evaluated its accuracy for detection of acute Chagas disease (CD) in different epidemiological and clinical scenarios. In this retrospective study, convenience series samples (venous blood treated with EDTA or stabilizer agents, heel-prick filter paper cerebrospinal fluid (CSF)) from 30 infants born to seropositive mothers (13 congenital CD 17 noninfected),...
BackgroundCurrent algorithm for Congenital Chagas Disease (cCD) diagnosis is unsatisfactory due to low sensitivity of the parasitological methods. Moreover, loss follow-up precludes final serodiagnosis after nine months life in many cases. A duplex TaqMan qPCR kit Trypanosoma cruzi DNA amplification was prospectively evaluated umbilical cord (UCB) and peripheral venous blood (PVB) infants born CD mothers at endemic non-endemic sites Argentina.MethodsWe enrolled followed-up 370 infants;...
Congenital infection of Trypanosoma cruzi allows transmission this parasite through generations. Despite the problematic that entails, little is known about placenta environment genetic response produced against infection. We performed functional genomics by microarray analysis in C57Bl/6J mice comparing placentas from uninfected animals and infected with two different T. strains: K98, a clone non-lethal myotropic CA-I strain (TcI), VD (TcVI), isolated human case congenital Analysis networks...
Background Trypanosoma cruzi , the hemoparasite that causes Chagas disease, is divided into six Discrete Typing Units or DTUs: TcI-TcVI plus Tcbat. This genetic diversity based on ecobiological and clinical characteristics associated with particular populations of parasite. The main objective this study was identification DTUs in patients chronic chagasic infections from a mountainous rural community eastern region Panama. Methods A total 106 were tested for disease three serological tests...
Abstract This study evaluated the effectiveness of low doses benznidazole (BNZ) on continuous administration (BNZc), combined with allopurinol (ALO), in C57BL/6J and C3H/HeN mice infected Trypanosoma cruzi Nicaragua strain T. Sylvio-X10/4 clone. Tc N-C57BL/6J was also treated intermittent BNZ (BNZit). The drug therapy started 3 months post infection (pi) chronic phase heart disease progression, followed-up at 6 pi. BNZc monitored up to 12 pi by serology electrocardiogram. These showed severe...
Introduction There are no reports in LATAM related to longitudinal humoral and cellular response adenovirus based COVID-19 vaccines people with Multiple Sclerosis (pwMS) under different disease modifying therapies (DMTs) neutralization of the Omicron Wuhan variants SARS-COV-2. Methods IgG anti- SARS-COV-2 spike titer were measured a cohort 101 pwMS fingolimod, dimethyl fumarate, cladribine antiCD20, as well 28 healthy controls (HC) 6 weeks after vaccination 2 nd dose (Sputnik V or AZD1222) 3...
dimensional reconstruction seen from the underside of human epidermis, generated immunohistochemistry whole skin biopsy samples obtained at 7 days after wounding.Cyan represents pilosebaceous units; magenta, eccrine sweat glands; yellow, new epidermis; gray, mesh for contours.