A5042730876- Hematopoietic Stem Cell Transplantation
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Blood groups and transfusion
- Immune Response and Inflammation
- Multiple Myeloma Research and Treatments
- Biomedical Ethics and Regulation
- Acute Myeloid Leukemia Research
- Platelet Disorders and Treatments
- Blood transfusion and management
- Organ Donation and Transplantation
- Complement system in diseases
- Cytomegalovirus and herpesvirus research
- Chronic Myeloid Leukemia Treatments
- Monoclonal and Polyclonal Antibodies Research
- Central Venous Catheters and Hemodialysis
- Hemoglobinopathies and Related Disorders
- Fatty Acid Research and Health
- Neonatal Respiratory Health Research
- Telomeres, Telomerase, and Senescence
- Birth, Development, and Health
- Prenatal Substance Exposure Effects
- Renal Diseases and Glomerulopathies
- Lymphoma Diagnosis and Treatment
University of Iowa
2016-2025
University of Iowa Health Care
2024
University of Iowa Hospitals and Clinics
1998-2021
Emory University
2013
Children's Healthcare of Atlanta
2013
University of Nebraska Medical Center
2007
Luther University
2002
Martin Luther University Halle-Wittenberg
2002
University of Illinois Urbana-Champaign
1989-1994
Illinois College
1994
Background: The well‐known immune deficiency of the chronic alcoholic dictates need for a long‐term rodent ethanol administration model to evaluate baseline immunologic effects abuse, and investigate genetic determinants those effects. Much published work with rodents has shown clearly that acute short‐term ethanol‐containing liquid diets both cause elevated corticosterone can significant thymocyte, pre‐B cell peripheral lymphocyte losses. Such losses may mask more subtle alterations in...
The risk of surgical site infection (SSI) after craniotomies or craniectomies in patients whom contaminated bone flaps have been reimplanted has not determined. objectives this study were to identify the prevalence with positive cultures--especially those Propionibacterium acnes--to assess SSI reimplanting (either during initial operation subsequently) cultures, and factors for following craniectomies.The authors conducted a retrospective review cases which underwent craniotomy/craniectomy...
Abstract Alcohol use by pregnant women is a significant public health issue despite well-described risks to the fetus including physical and intellectual growth retardation malformations. Although clinical studies are limited, they suggest that in utero alcohol exposure also results immune deficiencies naive neonates. However, little known about fetal (FAE) effects on adult infections. Therefore, determine long-term of FAE disease susceptibility system, we infected mice with influenza virus....
BACKGROUND ABO compatibility can affect platelet transfusion safety and efficacy, ABO‐incompatible (ABOi) platelets likely increases the risks of reactions though magnitude this risk is unclear. STUDY DESIGN AND METHODS Data collected on all transfusions administered over 36+ months were classified based patient product blood group type merged with a data set that included reported during period. The reaction rates among various subsets was calculated. RESULTS In patients greater than 1 year...
ABSTRACT Immune thrombotic thrombocytopenic purpura (iTTP) is characterized by microangiopathic hemolytic anemia, thrombocytopenia, and microvascular occlusion secondary to acquired ADAMTS13 deficiency. Contemporary data regarding iTTP treatment practices in the US, including use of caplacizumab, are lacking. We aimed characterize demographics therapies, medications apheresis practices, patients with US. retrospectively analyzed cases at 15 sites US that provide comprehensive care for iTTP....
Prior studies have suggested that immune thrombotic thrombocytopenic purpura (iTTP) may display seasonal variation; however, methodologic limitations and sample sizes diminished the ability to perform a rigorous assessment. This 5-year retrospective study assessed epidemiology of iTTP determined whether it displays pattern. Patients with both initial relapsed (defined as disintegrin metalloprotease thrombospondin type motifs 13 activity <10%) from 24 tertiary centers in Australia, Canada,...
Abstract Background The Ly‐6 family has many members, including Ly‐6C and Ly‐6G. A previous study suggested that the anti‐Ly‐6G antibody, RB6‐8C5, may react with hi murine bone marrow (BM) cells. This finding been interpreted as cross‐reactivity of RB6‐8C5 antigen, generalized to hematopoietic cell types, using terminology Ly‐6G/C. present was undertaken determine whether antibodies truly cross‐react antigen on multiple types. Methods Splenocytes, thymocytes, BM cells obtained from Ly‐6.1...
Background: Chronic alcoholics experience increased incidence and severity of infections, the mechanism which is incompletely understood. Dendritic cells (DC) migrate from peripheral locations to lymph nodes (LN) initiate adaptive immunity against infection. Little known about how chronic alcohol exposure affects skin DC numbers or migration. Methods: Mice received 20% EtOH in drinking water for up 35 weeks. Baseline Langerhans cell (LC) dermal (dDC) were enumerated by immunofluorescence...
Background: As initiators of immune responses, dendritic cells (DCs) are required for antigen (Ag)-specific activation naïve T in the defense against infectious agents. The increased susceptibility to and severity infection seen chronic alcoholics could be because impaired DCs initiation T-cell responses. Specifically, these may not provide adequate Signals 1 (Ag presentation), 2 (costimulation), or 3 (cytokine production) cells. Methods: Using Meadows-Cook murine model alcohol abuse,...
Abstract Membranoproliferative glomerulonephritis type II (MPGN II) is a rare kidney disease identified microscopically by electron‐dense deposits surrounded complement component C3 in glomerular basement membranes. MPGN usually leads to renal failure, and patients with experience high rate of recurrence following transplantation. No treatment modalities have been proven successful if does occur. The sera most contain nephritic factor (C3NF), an IgG autoantibody directed against convertase...
Pediatric emergency RBC transfusions are often infused rapidly through 22-gauge (ga) or smaller needles catheters using hand-held syringes. Data relating needle size, unit age, and infusion rate needed to assess the risk of hemolysis hyperkalemia in this setting.Multiple simulated were performed during storage units. Aliquots from five units passed (18, 20, 22-25 ga) a syringe. Resulting plasma Hb K+ concentrations measured. Free levels used as measure needle-associated (NAH).Passage 18-ga...
Chronic alcoholics have increased susceptibility to and severity of infection, which are likely be a result impaired immune defense mechanisms. The contribution dendritic cells (DC) these changes is not well understood. Alterations in DC numbers, dendropoiesis, lifespan been specifically studied vivo chronic ethanol (EtOH) exposure models. As play an essential role initiating responses, alterations characteristics would help explain observed adaptive responses.Mice received 20% EtOH (w/v)...
Background Chronic alcoholism is associated with increased incidence and severity of skin infection. Cutaneous dendritic cells (CDCs) play a pivotal role in immunity, chronic ethanol (EtOH) feeding mice has been shown to inhibit CDC migration skin-draining lymph nodes (dLNs) following epicutaneous sensitization. Because subsets differentially initiate T-cell responses, it important determine how EtOH affects each subset identify mechanisms responsible for observed defects. Methods Mice...
Background Chronic alcoholism is associated with increased incidence and severity of cutaneous infection. Skin‐resident T cells orchestrate numerous immunological functions that are critically involved in both tissue homeostasis immunity. The impact chronic ethanol (Et OH ) exposure on skin has not previously been examined; given their important role maintaining the immune barrier function further study warranted. Methods Mice were administered Et drinking water for 12 to 16 weeks. Flow...
Objective Sustained hemodynamic stress mediated by high blood flow promotes arteriogenesis, the outward remodeling of existing arteries. Here, we examined whether Ca2+/calmodulin-dependent kinase II (CaMKII) regulates arteriogenesis. Methods and Results Ligation left common carotid led to an increase in vessel diameter perimeter internal external elastic lamina contralateral, right carotid. Deletion CaMKIIδ (CaMKIIδ−/−) abolished this remodeling. Carotid ligation increased CaMKII expression...
Certain patients who receive granulocyte colony‐stimulating factor (GCSF) for autologous hematopoietic stem cell (AHSC) collection fail to mobilize well enough proceed with transplant. When plerixafor is used GCSF, the likelihood of achieving CD34 + target in fewer collections higher; use all unlikely be cost‐effective. This study retrospectively evaluated effectiveness utilizing a peripheral blood count (PBCD34) ≤8/µL on day 4 GCSF‐based AHSC mobilization as threshold administration, and...
Abstract Alcoholics are at increased risk of Staphylococcus aureus skin infection and serious sequelae, such as bacteremia death. Despite the association between alcoholism severe S. infection, impact EtOH on anti-S. cutaneous immunity has not been investigated in a model chronic exposure. To test hypothesis that enhances severity mice were fed for ≥12 weeks via Meadows-Cook inoculated with following epidermal abrasion. Evidence exacerbated staphylococcal disease EtOH-fed included: lesions...
Despite improvements in human leukocyte antigen matching and pharmacologic prophylaxis, acute graft-versus-host disease (GVHD) is often a fatal complication following hematopoietic stem cell transplant (HSCT). Older HSCT recipients experience significantly increased morbidity mortality compared to young recipients. Prophylaxis with syngeneic regulatory dendritic cells (DCreg) bone marrow transplanted (BMT) mice has been shown decrease GVHD-associated mortality. To evaluate this approach...
Hematopoietic progenitor cell (HPC) counts from Sysmex hematology analyzers have been shown to correlate with peripheral blood (PB) CD34+ by flow cytometry. Algorithms utilizing HPC guide stem collections proposed but rarely tested. This study describes the development and validation of algorithms PB CD34+cell predict adequate (PBSC) for chemomobilized cytokine-mobilized individuals.Utilizing a test set 83 samples or PBSC collection patients, were correlated receiver operating characteristic...
In patients with advanced colorectal cancer (CRC) refractory to systemic chemotherapy including 5-fluorouracil (5-FU) / folinic acid (FA), oxaliplatin and irinotecan we assessed the feasibility, toxicity response hepatic transcatheter arterial chemoembolization (TACE). At time of treatment, had exclusively or dominantly liver metastasis CRC.The following protocol was applied via a selective transfemoral approach: mitomycin C 5 mg/m(2), interferon-alpha2b 4.5 Mio IU, dexamethasone 20 mg mixed...
Abstract The expression patterns of the Ly-6C Ag were examined on splenic and thymic lymphocyte subsets Ly-6.1 Ly-6.2 strains mice using rat mAb 15.1. is expressed CD4+ CD8+ splenocytes, a portion NK cells. Within lymph node T cell compartment, restricted to mice, present subset naive majority peripheral cells in both strains, found primarily Ag-experienced subset. In thymus, subpopulations CD4- CD8+, CD8-, CD8- Ly-6C+ thymocytes show mature phenotype, while appear be part recently described...