A5038144016- CAR-T cell therapy research
- Cutaneous Melanoma Detection and Management
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Sarcoma Diagnosis and Treatment
- Nanoparticle-Based Drug Delivery
- Cellular Mechanics and Interactions
- Characterization and Applications of Magnetic Nanoparticles
- Force Microscopy Techniques and Applications
- Nanoparticles: synthesis and applications
- nanoparticles nucleation surface interactions
- Coagulation and Flocculation Studies
- Geomagnetism and Paleomagnetism Studies
- Melanoma and MAPK Pathways
- Single-cell and spatial transcriptomics
- Iron oxide chemistry and applications
- Particle Dynamics in Fluid Flows
- melanin and skin pigmentation
- Polymer composites and self-healing
- Thermography and Photoacoustic Techniques
- Thermal Radiation and Cooling Technologies
- Polydiacetylene-based materials and applications
- Hydrogels: synthesis, properties, applications
- Chemotherapy-related skin toxicity
- Cardiac tumors and thrombi
University of Fribourg
2016-2020
Adolphe Merkle Institute
2016-2020
Politecnico di Milano
2015
Fondazione IRCCS Istituto Nazionale dei Tumori
1996-2014
University of Milan
2014
Zimmer Biomet (United States)
2014
Genomics (United Kingdom)
2014
University of Milano-Bicocca
2014
Abstract As a highly interdisciplinary field, working with nanoparticles in biomedical context requires robust understanding of soft matter physics, colloidal behaviors, nano-characterization methods, biology, and bio-nano interactions. When reporting results, it can be easy to overlook simple, seemingly trivial experimental details. In this context, we set out understand how vitro technique, specifically the way administer particles 2D culture, influence outcomes. Gold coated...
Background: Surgical removal of axillary lymph node and histologic examination for metastases are used to determine whether adjuvant treatment is necessary patients with breast cancer. Axillary dissection (ALND) a costly procedure associated various side effects, 80% or more tumors 20 mm less negative might avoid ALND. In this study, we evaluated an alternative, noninvasive method—i.e., positron emission tomography (PET) 2-[18F]fluoro-2-deoxy-d-glucose (FDG)— could be status in Methods: One...
The purpose of the study was to evaluate excision margin necessary for desmoplastic melanoma (DM).DM consists 2 histologic subtypes, pure DM (PDM) and mixed (MDM), differing in extent fibrotic component. We investigated clinical therapeutic determinants prognosis these entities.We reviewed 118 PDM 124 MDM treated at our Institute over 25 years. Local relapse, distant metastasis, survival were studied.Most (91.7%) metastases developed after 1 or more local recurrences; whereas metastasis...
Sentinel lymph nodes set the stance of immune system to a localized tumor and are often first site be colonized by neoplastic cells that metastasize. To investigate how presence in sentinel may trigger pathways associated with metastatic progression, we analyzed transcriptional profiles archival node biopsy specimens obtained from melanoma patients. Biopsies positive were selected for comparable infiltration, or absence further regional metastases, relapse at 5-year follow-up. Unsupervised...
Magnetic hyperthermia has the potential to play an important role in cancer therapy and its efficacy relies on nanomaterials selected. Superparamagnetic iron oxide nanoparticles (SPIONs) are excellent candidates due ability of producing enough heat kill tumor cells by thermal ablation. However, their heating properties depend strongly crystalline structure size, which may not be controlled tuned during synthetic process; therefore, a postprocessing is needed. We show how annealing can...
We propose a new methodology based on lock-in thermography to study and quantify the heating power of magnetic nanoparticles. Superparamagnetic iron oxide nanoparticles exposed modulated alternating field were used as model materials demonstrate potency system. Both quantitative qualitative information their respective was extracted at high thermal resolutions under increasingly complex conditions, including in liquid, solid aggregated states. Compared conventional techniques, this approach...
The aim of this retrospective study was to analyze the incidence further nonsentinel node metastases at completion lymphadenectomy groin after a positive sentinel biopsy evaluate whether radical dissection remains treatment choice for these patients. Patients treated National Cancer Institute Milan between January 1999 and December 2006 were reviewed retrospectively. analysis included patients with diagnosis (clinically negative) who underwent groin, iliac, obturatory dissections. primary...
Magnetic nanoparticles and their ability to convert electromagnetic energy into heat are of explicit interest for various applications. However, precise quantification heating efficiency is not always upfront, several parameters render comparative studies challenging. This paper describes the theory behind lock-in thermography, a new technique quantifying properties magnetic nanoparticles. allows investigation some potential sources variability: key factors such as field inhomogeneity its...
Abstract: Nanoparticles (NPs) possess unique properties useful for designing specific functionalities biomedi- cal applications. A prerequisite of a safe-by-design and effective use in any biomedical application is to study NP–cell interactions gain better understanding cellular consequences upon exposure. Cellular uptake NPs results mainly the localization complex environment lysosomes, compartment which can be mimicked by artificial lysosomal fluid. In this work we showed applicability...
Users of dynamic light scattering (DLS) are challenged when a sample nanoparticles (NPs) contains dust. This is frequently inevitable scenario and major problem that critically affects the reproducibility accuracy DLS measurements. Current methods approach this via photon correlation spectroscopy, but remedy exists only for few special cases. We introduce here general criterion clearly defined measure to discriminate between NPs dust particles. The experimental results show that, in contrast...
Because of its high thickness, nodular melanoma often bears a poor prognosis. Thus, an earlier diagnosis this type lesion while it is still thin would be important step in secondary prevention. The principal aim the present study was to better define initial clinical features allow early diagnosis. A establish prognosis lesion.We retrospectively studied and illustrated 11 small (< or = 6 mm maximum diameter) cutaneous melanomas seen treated during 10-year period. Prognostic characteristics...
Magnetosomes are near-perfect intracellular magnetite nanocrystals found in magnetotactic bacteria. Their synthetic imitation, known as superparamagnetic iron oxide nanoparticles (SPIONs), have applications a variety of (nano)medicinal fields such magnetic resonance imaging contrast agents, multimodal and drug carriers. In order to perform these functions medicine, shape size control the SPIONs is vital. We sampled at ten-minutes intervals during high-temperature thermal decomposition...
Aims and backgroundBecause of its high thickness, nodular melanoma often bears a poor prognosis. Thus, an earlier diagnosis this type lesion while it is still thin would be important step ...
We perform a comparative study of dynamic force measurements using an Atomic Force Microscope (AFM) on the same soft polymer blend samples in both air and liquid environments. Our quantitative analysis starts with calibration cantilever Intermodulation AFM (ImAFM) is used to measure quadratures sample. validate accuracy reconstructed by numerical simulation realistic model liquid. In spite very low quality factor this resonance, we find excellent agreement between experiment simulation. A...
10593 Background: IM is active in DFSP by targeting PDGFRB that constitutively activated this rare low grade malignant tumor as a consequence of the chromosomal translocation t (17;22). Fibrosarcomatous transformation rarely occur DFSP, with an increased risk for metastasis. activity FS arising from less well defined. Methods: From June 2007, 2 patients metastatic high- (both men, age 45/75, PS 2) were treated 400 mg/day. Both cases have been investigated FISH analysis (17;22) and component....