A5091036060- Animal testing and alternatives
- Effects and risks of endocrine disrupting chemicals
- Zebrafish Biomedical Research Applications
- Computational Drug Discovery Methods
- Fungal and yeast genetics research
- Immune Cell Function and Interaction
- Carcinogens and Genotoxicity Assessment
- Environmental Toxicology and Ecotoxicology
- Immunotoxicology and immune responses
- Pharmaceutical and Antibiotic Environmental Impacts
- Pesticide Exposure and Toxicity
- Health Systems, Economic Evaluations, Quality of Life
- T-cell and B-cell Immunology
- Aquaculture disease management and microbiota
- Plant-Microbe Interactions and Immunity
- Drug-Induced Hepatotoxicity and Protection
- Toxic Organic Pollutants Impact
- Plant Disease Resistance and Genetics
- Drug Transport and Resistance Mechanisms
- Anesthesia and Neurotoxicity Research
- Thyroid Disorders and Treatments
- Molecular Biology Techniques and Applications
- 3D Printing in Biomedical Research
- Chemistry and Chemical Engineering
- Pharmacological Effects and Toxicity Studies
Humane Society of the United States
2013-2023
Dongguk University
2021
Campden BRI (United Kingdom)
2021
Park University
2021
Universidade Federal de Goiás
2021
People for the Ethical Treatment of Animals
2008-2012
Center for Science in the Public Interest (United States)
2008
Phylonix (United States)
2003-2008
National Institute of Environmental Health Sciences
2008
National Institutes of Health
2008
In zebrafish, the transparent and rapidly developing embryo potential for genetic screening offer a unique opportunity to investigate early development of vertebrate immune system. Here we describe initial appearance various blood lineages nature accumulating hematopoietic tissue in thymus kidney, main lymphoid organs adult teleosts. The ultrastructure first site hematopoiesis, intermediate cell mass (ICM), is described from 5-somite stage, about 11.5 hours post-fertilization (hpf) until 24...
We cloned and characterized three genes from Aspergillus nidulans, designated brlA, abaA, wetA, whose activities are required to complete different stages of conidiophore development. Inactivation these causes major abnormalities in morphology prevents expression many unrelated, developmentally regulated genes, without affecting the nonregulated genes. The code for poly(A)+ RNAs that begin accumulate at times during conidiation. brlA- abaA-encoded specifically cells conidiophore....
Abstract BACKGROUND: To establish zebrafish as a developmental toxicity model, we used 7 well‐characterized compounds to examine several parameters of neurotoxicity during development. METHODS: Embryos were exposed by semistatic immersion from 6 hrs postfertilization (hpf). Teratogenicity was assessed using modified method previously developed Phylonix. Dying cells in the brain acridine orange staining (these are likely be apoptotic). Motor neurons antiacetylated tubulin and...
Adverse outcome pathways (AOPs) have been recently introduced in human risk assessment as pragmatic tools with multiple applications. As such, AOPs intend to provide a clear-cut mechanistic representation of pertinent toxicological effects. are typically composed molecular initiating event, series intermediate steps and key events, an adverse outcome. In this study, AOP framework is proposed for cholestasis triggered by drug-mediated inhibition the bile salt export pump transporter protein....
Next generation risk assessment (NGRA) is an exposure-led, hypothesis-driven approach that has the potential to support animal-free safety decision-making. However, significant effort needed develop and test in vitro silico (computational) approaches underpin NGRA enable confident application a regulatory context. A workshop was held Montreal 2019 discuss where needs be focussed agree on steps ensure decisions made cosmetic ingredients are robust protective. Workshop participants explored...
Our ability to conduct whole-organism toxicity tests understand chemical safety has been outpaced by the synthesis of new chemicals for a wide variety commercial applications. As result, scientists and risk assessors are turning mechanistically based studies increase efficiencies in assessment making greater use vitro silico methods evaluate potential environmental human health hazards. In this context, adverse outcome pathway (AOP) framework gained traction regulatory science because it...
Abstract The Ikaros gene encodes a transcription factor that, in mice, has been shown to be essential for the correct differentiation of B and T lymphocytes is expressed all cells lymphoid lineage, including pluripotent hematopoietic stem cells. During development zebrafish, expression begins lateral mesoderm, continues intermediate cell mass (ICM), which derived from mesoderm generate primitive precursors. Cells expressing were then seen on ventral side dorsal aorta, known location...
Binding sites for three distinct proteins were mapped within the upstream activation (UAS) of yeast enolase genes ENO1 and ENO2. Sequences that overlapped UAS1 elements both bound a protein which was identified as product RAP1 regulatory gene. UAS2 element ENO2 gene second corresponded to ABFI (autonomously replicating sequence-binding factor) protein. A designated EBF1 (enolase-binding sequences in ENO1. There good correlation among all factor-binding location required UAS activity by...
Biomedical developments in the 21st century provide an unprecedented opportunity to gain a dynamic systems-level and human-specific understanding of causes pathophysiologies disease. This is vital need, view continuing failures health research, drug discovery, clinical translation. The full potential advanced approaches may not be achieved within 20th-century conceptual framework dominated by animal models. Novel technologies are being integrated into environmental research also applicable...
Abstract Zebrafish ( Danio rerio ) has been extensively studied and well described for environmental toxicity studies. Molecular biology genetics have recently used to elucidate the underlying mechanisms of in zebrafish predict effects mammals. The versatile is now incorporated many areas toxicological programs assessing human risk preclinical drug discovery screening.
We cloned and characterized three genes from Aspergillus nidulans, designated brlA, abaA, wetA, whose activities are required to complete different stages of conidiophore development. Inactivation these causes major abnormalities in morphology prevents expression many unrelated, developmentally regulated genes, without affecting the nonregulated genes. The code for poly(A)+ RNAs that begin accumulate at times during conidiation. brlA- abaA-encoded specifically cells conidiophore....
A major challenge in regulatory developmental neurotoxicity (DNT) assessment is lack of toxicological information for many compounds. Therefore, the Test Guidelines programme Organisation Economic Cooperation and Development (OECD) took initiative to coordinate an international collaboration between diverse stakeholders consider integration alternative approaches towards improving current chemical DNT testing. During past few years, a series workshops was organized during which consensus...
The development of more efficient, ethical, and effective means assessing the effects chemicals on human health environment was a lifetime goal Gilman Veith. His work has provided foundation for use chemical structure informing toxicological assessment by regulatory agencies world over. Veith's scientific influenced early SAR models in today at US Environmental Protection Agency. He driving force behind Organisation Economic Co-operation Development QSAR Toolbox. Veith one few pioneers whose...
Adverse Outcome Pathways (AOP) provide structured frameworks for the systematic organization of research data and knowledge. The AOP framework follows a set key principles that allow broad application across diverse disciplines related to human health, including toxicology, pharmacology, virology medical research. COVID-19 pandemic engages great number scientists world-wide is increasing with exponential speed. Diligent management strategies are employed but approaches systematically...
New approaches to generating and evaluating toxicity data for chemicals are needed cope with the ever-increasing demands of new programs. One such approach involves use an integrated testing evaluation strategy based on specific properties activities a chemical. Such strategy, whether applied existing or future programs, can promote efficient resources save animals. We demonstrate utility by applying it current U.S. Environmental Protection Agency Endocrine Disruptor Screening Program...