A5000817606- Neuroscience and Neuropharmacology Research
- Calcium signaling and nucleotide metabolism
- Photoreceptor and optogenetics research
- Adipokines, Inflammation, and Metabolic Diseases
- Cell Image Analysis Techniques
- Adenosine and Purinergic Signaling
- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- Memory and Neural Mechanisms
- Receptor Mechanisms and Signaling
- Curcumin's Biomedical Applications
- Calpain Protease Function and Regulation
- Alzheimer's disease research and treatments
- Nicotinic Acetylcholine Receptors Study
- Epilepsy research and treatment
- Cardiovascular Function and Risk Factors
- Cardiovascular and exercise physiology
- Biochemical Analysis and Sensing Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Renal function and acid-base balance
- Inhalation and Respiratory Drug Delivery
- Nanoparticle-Based Drug Delivery
- Ion Channels and Receptors
- Cell death mechanisms and regulation
- Neural dynamics and brain function
University of Milano-Bicocca
2018-2022
Fondazione IRCCS Istituto Neurologico Carlo Besta
2018
The neurotransmitter glutamate increases cerebral blood flow by activating postsynaptic neurons and presynaptic glial cells within the neurovascular unit. Glutamate does so causing an increase in intracellular Ca 2+ concentration ([Ca ] i ) target cells, which activates /Calmodulin‐dependent nitric oxide (NO) synthase to release NO. It is unclear whether brain endothelial also sense through elevation [Ca NO production. current study assessed how drives ‐dependent bEND5 established model of...
Abstract Basal forebrain neurons control cerebral blood flow (CBF) by releasing acetylcholine (Ach), which binds to endothelial muscarinic receptors induce nitric (NO) release and vasodilation in intraparenchymal arterioles. Nevertheless, the mechanism whereby Ach stimulates human brain microvascular cells produce NO is still unknown. Herein, we sought assess whether production a Ca 2+ ‐dependent manner hCMEC/D3 cells, widespread model of cells. induced dose‐dependent increase intracellular...
The neuromodulator histamine is able to vasorelax in human cerebral, meningeal and temporal arteries via endothelial 1 receptors (H1 Rs) which result the downstream production of nitric oxide (NO), most powerful vasodilator transmitter brain. Although Ca 2+ signals drive histamine-induced NO release throughout peripheral circulation, mechanism by evokes cerebrovascular cells still unknown. Herein, we exploited cerebral microvascular cell line, hCMEC/D3, assess role intracellular signaling...
Summary Objective The key factors that promote the termination of focal seizures have not been fully clarified. buildup neuronal synchronization during has proposed as one possible activity‐dependent, self‐limiting mechanisms. We investigate if increased thalamo‐cortical coupling contributes to enhance late phase seizure‐like events ( SLE s) generated in limbic regions. Methods Recordings were simultaneously performed nucleus reuniens thalamus, hippocampus and entorhinal cortex isolated...
We have uncovered a novel role for astrocytes-derived extracellular vesicles (EVs) in controlling intraneuronal Ca2+ concentration ([Ca2+]i) and identified transglutaminase-2 (TG2) as surface-cargo of EVs. Incubation hippocampal neurons with primed astrocyte-derived EVs led to an increase [Ca2+]i, unlike from TG2-knockout astrocytes. Exposure or brain slices TG2 promoted [Ca2+]i rise, which was reversible upon removal dependent on influx through the plasma membrane. Patch-clamp calcium...
Oxygen diffusion across the air-blood barrier in lung is commensurate with metabolic needs and ideally allows full equilibration between alveolar blood partial oxygen pressures. We estimated alveolo-capillary O2 18 healthy subjects at sea level rest after exposure to increased demand, including work on hypobaric hypoxia 3840 m (PA ~ 50 mmHg). For each subject we capacity (DO2), pulmonary capillary volume (Vc) cardiac output ([Formula: see text]). derived transit time [Formula: text] constant...
Abstract In light of previous results, we assessed whether liposomes functionalized with ApoE-derived peptide (mApoE) and phosphatidic acid (PA) (mApoE-PA-LIP) impacted on intracellular calcium (Ca 2+ ) dynamics in cultured human cerebral microvascular endothelial cells (hCMEC/D3), as an vitro blood-brain barrier (BBB) model, astrocytes. mApoE-PA-LIP pre-treatment actively increased both the duration area under curve (A.U.C) ATP-evoked Ca waves hCMEC/D3 well even 0 [Ca ] e conditions, thus...
Abstract We have uncovered a novel role for astrocytes-derived extracellular vesicles (EVs) in controlling intraneuronal Ca 2+ concentration ([Ca ] i ) and identified transglutaminase-2 (TG2) as surface-cargo of EVs. Incubation hippocampal neurons with primed astrocyte-derived EVs led to an increase [Ca , unlike from TG2-knockout astrocytes. Exposure or brain slices TG2 promoted rise, which was reversible upon removal dependent on influx through the plasma membrane. Patch-clamp calcium...