A5088120229- Blood properties and coagulation
- Advanced Proteomics Techniques and Applications
- Immune Cell Function and Interaction
- Erythrocyte Function and Pathophysiology
- PARP inhibition in cancer therapy
- CAR-T cell therapy research
- Cancer-related molecular mechanisms research
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Acute Myeloid Leukemia Research
- Mass Spectrometry Techniques and Applications
- Mesenchymal stem cell research
- Bone Metabolism and Diseases
- Gene expression and cancer classification
- vaccines and immunoinformatics approaches
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Extracellular vesicles in disease
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer, Hypoxia, and Metabolism
- Probiotics and Fermented Foods
- Cancer-related Molecular Pathways
- Free Radicals and Antioxidants
- Building materials and conservation
- Tuberous Sclerosis Complex Research
- Diagnosis and Treatment of Venous Diseases
Nottingham Trent University
2014-2024
Abstract Pentose phosphate pathway (PPP) is a major glucose metabolism pathway, which has fundamental role in cancer growth and metastasis. Even though PPP blockade been pointed out as very promising strategy against cancer, effective anti-PPP agents are not still available the clinical setting. Here we demonstrate that natural molecule polydatin inhibits glucose-6-phosphate dehydrogenase (G6PD), key enzyme of PPP. Polydatin blocks G6PD causing accumulation reactive oxygen species strong...
Ataxia-telangiectasia mutated (ATM), ataxia-telangiectasia and rad3 related (ATR) DNA-dependent protein kinase catalytic sub-unit (DNA-PKcs) play critical roles in DNA damage response (DDR) by linking sensing to DDR effectors that regulate cell cycle progression repair. Our objective was evaluate if ATM, ATR DNA-PKcs expressions could predict therapy clinical outcome epithelial ovarian cancers. We investigated ATR, cancers [protein expression (n = 194 patients), mRNA 156 patients)]...
Increased export of transglutaminase-2 (TG2) by tubular epithelial cells (TECs) into the surrounding interstitium modifies extracellular homeostatic balance, leading to fibrotic membrane expansion. Although silencing TG2 ameliorates progressive kidney scarring in animal models CKD, pathway through which is secreted from TECs and contributes disease progression has not been elucidated. In this study, we developed a global proteomic approach identify binding partners responsible for...
Introduction In the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed “pollensomes”, are released by pollen during germination. These vesicles (EVs) may play an important role in pollen-pistil interaction fertilization, stabilizing secreted bioactive molecules and allowing long-distance signaling. However, molecular composition biological of these EVs still unclear. The present study had two main aims: (I) to clarify whether germination is needed...
The rat sub-total nephrectomy (SNx) is a functional model of general chronic kidney disease (CKD) where the main pathological driver glomerular hypertension representative several subtypes CKD. Comprehensive transcriptomics and proteomics analyses on SNx rats were performed to identify biomarkers in plasma or urine that correlate with loss. Kidneys subjected collagen I III staining for fibrosis scoring, SWATH-MS bulk RNA-sequencing transcriptomics, also urine. Differential expression...
We have used proteomic fingerprinting to investigate diagnosis of Alzheimer's disease (AD). Samples lumbar cerebrospinal fluid (CSF) from clinically-diagnosed AD cases (n = 33), age-matched controls 20), and mild cognitive impairment (MCI) patients 10) were obtain profiles, followed by bioinformatic analysis that generated a set potential biomarkers in CSF samples could discriminate controls. The identity the biomarker ions was determined using mass spectroscopy. panel seven peptide able...
Metastasis is associated with poor prognosis in breast cancer. Although some studies suggest beta-blockers increase survival by delaying metastasis, others have been discordant. This study provides both insights into the anomalous findings and identifies potential biomarkers that may be treatment targets. Cell line models of basal-type oestrogen receptor-positive cancer were profiled for basal levels adrenoceptor gene/protein expression, β2-adrenoceptor mediated cell behaviour including...
Since the 'rediscovery' of Selden map China, an early seventeenth century Asia, in Bodleian Library Oxford, importance our understanding globalisation has been recognised. One unresolved questions is origin map. This paper addresses question through material evidence provided by a holistic approach using suite complementary analytical techniques. The was examined situ and non-invasively remote spectral imaging instrument (PRISMS) modified for close range imaging, which followed techniques...
The need for anticancer therapies that overcome metallodrug resistance while minimizing adverse toxicities is targeted, herein, using titanium coordination complexes. Octahedral titanium(IV)
The importance of parental diet in relation to eventual offspring health is increasing prominence due the increased frequency parents reproductive age consuming poor diets. Whilst maternal and outcome have been studied some detail, paternal impacts are not as well understood. A father's nutritional status has shown negative consequences on foetal growth development ultimately impact long-term adult offspring. In this study, we examined sperm- seminal vesicle fluid-mediated mechanisms...
We have uncovered a novel role for astrocytes-derived extracellular vesicles (EVs) in controlling intraneuronal Ca2+ concentration ([Ca2+]i) and identified transglutaminase-2 (TG2) as surface-cargo of EVs. Incubation hippocampal neurons with primed astrocyte-derived EVs led to an increase [Ca2+]i, unlike from TG2-knockout astrocytes. Exposure or brain slices TG2 promoted [Ca2+]i rise, which was reversible upon removal dependent on influx through the plasma membrane. Patch-clamp calcium...
Lymphangioleiomyomatosis (LAM) is a rare disease of women. Decline in lung function variable, making appropriate targeting therapy difficult. We used unbiased serum proteomics to identify markers associated with outcome LAM.101 women LAM and 22 healthy controls were recruited from the National Centre for UK. 152 DNA samples linked data obtained patients Heart, Lung Blood Institute Registry USA. Proteomic analysis was performed on discovery cohort 50 20 control using SCIEX SWATH mass...
Amyloid-beta (Aβ) deposition in the brain is closely linked with development of Alzheimer's disease (AD). Unfortunately, therapies specifically targeting Aβ have failed to reach their primary clinical endpoints, emphasizing need broaden search strategy for alternative targets/mechanisms. Transglutaminase-2 (TG2) catalyzes post-translational modifications, present AD lesions and interacts AD-associated proteins. However, an unbiased overview TG2 interactors lacking both control brain. Here we...
Abstract Background Natural killer (NK) cells originate from bone marrow precursors and mediate effective anti-tumor responses. Clinical trials of cytokine-primed memory-like (ML) NK in acute myeloid leukemia (AML) have demonstrated activity without major toxicity, including graft-versus-host disease or cytokine release syndrome. However, broad application non-expanded, non-engineered ML has been hindered by limited availability a single donor, thereby precluding aggressive dose escalation...
Background Homocitrullination is the post-translational modification of lysine that recognized by T cells. Methods This study identified homocitrullinated peptides from aldolase, enolase, cytokeratin and binding immunoglobulin protein used human leukocyte antigen (HLA) transgenic mice to assess immunogenicity enzyme-linked immunosorbent spot assay. Vaccine efficacy was assessed in tumor therapy studies using HLA-matched B16 melanoma expressing constitutive or interferon γ (IFNγ)-inducible...
Raw spectral data from matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) with MS profiling techniques usually contains complex information not readily providing biological insight into disease. The association of identified features within raw to a known peptide is extremely difficult. Data preprocessing remove uncertainty characteristics in the normally required before performing any further analysis. This study proposes an alternative yet simple solution preprocess...
Treatment of gestational trophoblastic diseases (GTD) involves surgery, radiotherapy and chemotherapy. Although, these therapeutic approaches are highly successful, drug resistance toxicity remain a concern for high risk patients. This Chemoresistance has also been observed in the presence cancer stem cells that thought to be responsible cases recurrence. In this study, we report previously unknown populations stem-like (SLCs) resistant chemotherapeutic doxorubicin. We demonstrate express...
The p53 protein is mutated in more than 50% of human cancers. Mutated proteins not only lose their normal function but often acquire novel oncogenic functions, a phenomenon termed mutant gain-of-function. Mutant has been shown to affect the transcription range genes, as well protein-protein interactions with factors and other effectors; however, no one intensively investigated identified these proteins, or MHC presented epitopes, from viewpoint ability act targets for immunotherapeutic...
To advance our understanding of mechanisms involved in tumor progression/regression, a CT26 colorectal mouse model treated intra-tumorally with DISC-herpes simplex virus as immunotherapy was used the discovery and validation phases to investigate ultimately identify biomarkers correlating failure respond immunotherapy.For phase, serum protein/peptide profiles retrospective sample collection (total n=70) were analyzed using MALDI-TOF-MS combined artificial neural networks. Following...
Abstract Exercise is a non-pharmacological intervention that can enhance bone regeneration and improve the management of conditions like osteoporosis or metastatic cancer. Therefore, it gaining increasing importance in an emerging area regenerative medicine—regenerative rehabilitation (RR). Osteocytes are mechanosensitive secretory cells orchestrate anabolism hence postulated to be attractive target exercise interventions. However, human osteocyte signalling pathways processes evoked upon...
The present study was conducted to investigate the stimbiotic mechanism of xylo-oligosaccharide (XOS) in degrading complex polysaccharides by caecal bacteria chicken, applying a proteomic approach. A total 800 as-hatched Ross 308 broiler chicks were equally divided into 4 experimental pens (200 per pen) at commercial poultry barn, allocating 2 treatment. Birds fed ad libitum with dietary treatments; CON (without XOS) and XOS (with 0.1g XOS/kg diet) from d 0 35. From each pen, 60 Individual...
The expected five-year survival rate from a stage III ovarian cancer diagnosis is mere 22%; this applies to the 7000 new cases diagnosed yearly in UK. Stratification of patients with heterogeneous disease, based on active molecular pathways, would aid targeted treatment improving prognosis for many cases. While hundreds genes have been associated cancer, few yet verified by peer research clinical significance. Here, meta-analysis approach was applied two carefully selected gene expression...