A5041984103- Stress Responses and Cortisol
- RNA Research and Splicing
- Tryptophan and brain disorders
- Genomics and Rare Diseases
- Diet and metabolism studies
- Neuroendocrine regulation and behavior
- MicroRNA in disease regulation
- Gut microbiota and health
- RNA modifications and cancer
- Genetic Associations and Epidemiology
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Genetics and Neurodevelopmental Disorders
- Cancer-related molecular mechanisms research
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Linguistics and Discourse Analysis
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Gene expression and cancer classification
- Atherosclerosis and Cardiovascular Diseases
- Health Systems, Economic Evaluations, Quality of Life
- Genomics and Chromatin Dynamics
- Dental Research and COVID-19
- Infection Control and Ventilation
University Medical Center of the Johannes Gutenberg University Mainz
2020-2024
Johannes Gutenberg University Mainz
2019-2024
ORCID
2020-2021
Fresenius (Germany)
2018-2020
Institute of Human Genetics
2020
Goethe University Frankfurt
2017
Sofia University "St. Kliment Ohridski"
1968
Atherosclerotic plaque development depends on chronic inflammation of the arterial wall. A dysbiotic gut microbiota can cause low-grade inflammation, and composition was linked to cardiovascular disease risk. However, role this environmental factor in atherothrombosis remains undefined. To analyze impact atherothrombosis, we rederived low-density lipoprotein receptor-deficient (Ldlr-/- ) mice as germfree (GF) kept these for 16 weeks an atherogenic high-fat Western diet (HFD) under GF...
Advances in computational power have enabled research to generate significant amounts of data related complex biological problems. Consequently, applying appropriate analysis techniques has become paramount tackle this complexity. However, theoretical understanding statistical methods is necessary ensure that the correct method used and sound inferences are made based on analysis. In article, we elaborate theory behind principal components (PCA), which a favoured multivariate tool field...
Although mortality after cardiac surgery has significantly decreased in the last decade, patients still experience clinically relevant postoperative complications. Among others, atrial fibrillation (AF) is a common consequence of surgery, which associated with prolonged hospitalization and increased mortality.We retrospectively analyzed data from who underwent coronary artery bypass grafting, valve or combination both at University Hospital Muenster between April 2014 July 2015. We evaluated...
α-Linolenic acid (ALA) is well-known for its anti-inflammatory activity. In contrast, the influence of an ALA-rich diet on intestinal microbiota composition and impact small intestine morphology are not fully understood. current study, we kept adult C57BL/6J mice 4 weeks or control diet. Characterization microbial revealed that ALA was associated with enrichment in Prevotella Parabacteroides. taxa belonging to Firmicutes phylum, including Lactobacillus, Clostridium cluster XIVa,...
The gut microbiota affects remote organ functions but its impact on organotypic endothelial cell (EC) transcriptomes remains unexplored. liver endothelium encounters microbiota-derived signals and metabolites via the portal circulation. To pinpoint how commensals affect hepatic sinusoidal endothelium, a magnetic sorting protocol, combined with fluorescence-activated sorting, was used to isolate ECs from germ-free (GF) conventionally raised (CONV-R) mice for transcriptome analysis by RNA...
Anandamide (AEA) is an endogenous ligand of the cannabinoid CB1 and CB2 receptors, being a component endocannabinoid signaling system, which supports maintenance or regaining neural homeostasis upon internal external challenges. AEA thought to play protective role against development pathological states after prolonged stress exposure, including depression generalized anxiety disorder. Here, we used chronic social defeat (CSD) as ethologically valid model in male mice. We characterized...
Alzheimer's disease (AD) is the most common form of dementia. Besides its cognitive phenotype, AD leads to crucial changes in gut microbiome composition model mice and patients, but reported data are still highly inconsistent. Therefore, we investigated chronic effects AD-characteristic neurotoxic amyloid-β (Aβ) peptides as provided by transgenic overexpression (5xFAD mouse model) acute due oral application Aβ on microbes. Astonishingly, one-time feeding wild type with Aβ42 provoked...
Abstract Background Due to exposure potentially infectious aerosols during treatments, the dental personnel is considered being at high risk for aerosol transmitted diseases like COVID-19. The aim of this study was evaluate different treatments as well efficacy suction reduce spreading. Methods Dental powder-jet (PJ; Air-Flow ® ), a water-cooled handpiece with diamond bur (HP) and ultrasonic scaling (US) were used in simulation head, mounted on unit various treatment settings. influence use...
Background Small sample sizes combined with multiple correlated endpoints pose a major challenge in the statistical analysis of preclinical neurotrauma studies. The standard approach applying univariate tests on individual response variables has advantage simplicity interpretation, but it fails to account for covariance/correlation data. In contrast, multivariate techniques might more adequately capture multi-dimensional pathophysiological pattern and therefore provide increased sensitivity...
The microbiota has been linked to the development of atherosclerosis, but functional impact these resident bacteria on lesion size and cellular composition atherosclerotic plaques in aorta never experimentally addressed with germ-free low-density lipoprotein receptor-deficient (Ldlr-/- ) mouse atherosclerosis model. Here, we report that 16 weeks high-fat diet (HFD) feeding hypercholesterolemic Ldlr-/- mice at (GF) housing conditions did not relative aortic root plaque size, macrophage...
Background and Purpose Post‐traumatic stress disorder ( PTSD ) is a heterogeneous induced by trauma, resulting in severe long‐term impairments of an individual's mental health. does not develop every individual and, thus, some individuals are more resilient. However, the underlying molecular mechanisms poorly understood. Here, we aimed to elucidate these processes. Experimental Approach We used single‐trauma model mice induce maladaptive behaviours profiled 4 weeks after trauma into...
Chronic social defeat (CSD) can lead to impairments in interaction and other behaviors that are supposed model features of major depressive disorder (MDD). Not all animals subjected CSD, however, develop these impairments, maintained some is widely used as a for resilience stress-induced mental dysfunctions. So far, have mainly been studied shortly (24 hours 7 days) after CSD exposure longitudinal development behavioral phenotypes individual has mostly neglected. We analyzed novel object...
Abstract Motivation Gene expression and alternative splicing are strictly regulated processes that shape brain development determine the cellular identity of differentiated neural cell populations. Despite availability multiple valuable datasets, many functional implications, especially those related to splicing, remain poorly understood. Moreover, neuroscientists working primarily experimentally often lack bioinformatics expertise required process data produce meaningful interpretable...
Abstract microRNAs are crucial regulators of brain development, however, miRNA regulatory networks not sufficiently well characterized. By performing small RNA-seq the mouse embryonic cortex at E14, E17, and P0 as in neural progenitor cells neurons, here we detected clusters miRNAs that were co-regulated distinct developmental stages. such miR-92a/b acted hubs during early, miR-124 miR-137 late neurogenesis. Notably, validated targets hub enriched for downregulated genes related to stem cell...
Summary While random X-chromosome inactivation in female cells of placental mammalians silences one allele the majority X-chromosomal genes, a considerable fraction is only incompletely and variably inactivated resulting tissue-specific pattern mono- biallelic expression. Here we used clonal human induced pluripotent stem (iPSCs) allowing to trace (in)activation status two X-chromosomes individually along neural differentiation trajectories. We discovered X-chromosome-wide locus-...
Upon chronic stress, a fraction of individuals shows stress resilience, which can prevent long-term mental dysfunction. The underlying molecular mechanisms are complex and have not yet been fully understood. In this study, we performed data-driven behavioural stratification together with single-cell transcriptomics the hippocampus in mouse model social defeat stress. Our work revealed that sub-group exhibiting responses upon dorsal is particularly involved neuroimmune responses,...
Abstract The mammalian brain consists of several structurally and functionally distinct regions equipped with an equally complex cell-type system. Due to its relevance in uncovering disease mechanisms, the study cell-type-specific molecular signatures different has increased. rapid evolution newer cheaper sequencing techniques also boosted interest epigenetic studies. In fact, nucleus holds most cell’s information is quite resistant tissue dissociation processes as compared cells. As such,...
Abstract Background Next Generation Sequencing (NGS) is the fundament of various studies, providing insights into questions from biology and medicine. Nevertheless, integrating data different experimental backgrounds can introduce strong biases. In order to methodically investigate magnitude systematic errors in single nucleotide variant calls, we performed a cross-sectional observational study on genomic cohort 99 subjects each sequenced via (i) Illumina HiSeq X, (ii) HiSeq, (iii) Complete...
Physical activity is considered a promising preventive intervention to reduce the risk of developing Alzheimer’s disease (AD). However, positive effect therapeutic administration physical has not been proven conclusively yet, likely due confounding factors such as varying regimens and life or stages. To examine impact different routines in early stages, we subjected young 5xFAD wild-type mice 1-day (acute) 30-day (chronic) voluntary wheel running compared them with age-matched sedentary...
Abstract Summary Animal behavioral studies typically generate high-dimensional datasets consisting of multiple correlated outcome measures across distinct or related domains. Here, we introduce the BEhavioral Explorative analysis R shiny APP (beeRapp) that facilitates explorative and inferential data in a high-throughput fashion. By employing an intuitive user-friendly graphical user interface, beeRapp empowers scientists without programming science expertise to perform clustering,...
Trait anxiety is a major risk factor for stress-induced and disorders in humans. However, animal models accounting the interindividual variability stress vulnerability are largely lacking. Moreover, pervasive bias of using mostly male animals preclinical studies poorly reflects increased prevalence psychiatric women. Using threat imminence continuum theory, we designed validated an auditory aversive conditioning-based pipeline both female mice. We operationalised trait by harnessing...
Gene expression and alternative splicing are strictly regulated processes that shape brain development determine the cellular identity of differentiated neural cell populations. Despite availability multiple valuable datasets, many functional implications, especially those related to splicing, remain poorly understood. Moreover, neuroscientists working primarily experimentally often lack bioinformatics expertise required process data produce meaningful interpretable results. Notably,...
RBFOX2 is an RNA-binding protein crucial for alternative splicing regulation and implicated in several neurodevelopmental disorders. Here, we show that while present only at low levels neural progenitor cells (NPCs), it upregulated differentiating neurons of the mouse embryonic neocortex. In-utero-electroporation-induced overexpression resulted a cellular phenotype characterized by impaired neuronal migration differentiation. Genome-wide analysis E15.5 revealed numerous events associated...
<title>Abstract</title> Susceptibility to chronic social stressors often results in the development of mental health disorders including major depressive and anxiety disorders. In contrast, some individuals remain resilient even after repeated stress exposure. Understanding molecular drivers behind these divergent phenotypic outcomes is crucial. However, previous studies using defeat (CSD) model have been limited by use bulk tissues investigating single omics domains. To overcome...
Abstract Chronic social defeat (CSD) can lead to impairments in interaction and other behaviors that are supposed model features of major depressive disorder (MDD). Not all animals subjected CSD, however, develop these impairments, maintained some is widely used as a for resilience stress-induced mental dysfunctions. So far, have mainly been studied shortly (24 hours 7 days) after CSD exposure longitudinal development behavioral phenotypes individual has mostly neglected. We analyzed novel...