A5100608980- Metabolism and Genetic Disorders
- Alcoholism and Thiamine Deficiency
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- Aluminum toxicity and tolerance in plants and animals
- Biochemical Acid Research Studies
- Trace Elements in Health
- Cholinesterase and Neurodegenerative Diseases
- Diet and metabolism studies
- Eicosanoids and Hypertension Pharmacology
- Infectious Encephalopathies and Encephalitis
- Neurological and metabolic disorders
- Adipose Tissue and Metabolism
- Bone Metabolism and Diseases
- GABA and Rice Research
- Biochemical effects in animals
- Medicinal Plants and Neuroprotection
- 14-3-3 protein interactions
- Peroxisome Proliferator-Activated Receptors
- Carcinogens and Genotoxicity Assessment
- Cancer, Stress, Anesthesia, and Immune Response
- Sirtuins and Resveratrol in Medicine
- Neurological Disorders and Treatments
- Tryptophan and brain disorders
Gdańsk Medical University
2010-2023
Institute of Labour and Social Studies
2017
Laboratoire de Biochimie
2005
Stellenbosch University
2005
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2005
Poznan University of Medical Sciences
1997-2004
Chronic hyperglycemia contributes to vascular complications in diabetes. Resveratrol exerts anti-diabetic and anti-platelet action. This study aimed evaluate the effects of resveratrol on metabolism function blood platelets under static vitro flow conditions patients with type 2 Blood obtained from 8 healthy volunteers 10 diabetes was incubated perfused over collagen-coated capillaries. Isolated were activated by collagen assess platelet function, metabolism, ATP release, TXA2 production,...
Most inflammatory bowel diseases (IBDs) are classic complex disorders represented by common alleles. Here we aimed to define the genetic architecture of pediatric and adult-onset IBDs for Polish population. A total 1495 patients were recruited, including 761 with Crohn's disease (CD; 424 pediatric), 734 ulcerative colitis (UC; 390 934 healthy controls. Allelotyping employed a pooled-DNA genome-wide association study (GWAS) was validated individual genotyping. Whole exome sequencing (WES)...
Increased activity of blood platelets contributes to vascular complications in patients with diabetes. The aim this work was investigate whether persisting hyperglycemia diabetic generates excessive accumulation ATP/ADP, which may underlie platelet hyperactivity. Platelet ATP and ADP levels, thiobarbituric acid–reactive species synthesis, aggregation from diabetes were 18–82% higher than healthy participants. In diabetes, stimulation thrombin caused about two times greater release the group...
J. Neurochem. (2010) 115 , 333–342. Abstract The decrease of pyruvate and ketoglutarate dehydrogenase complex activities is the main cause energy acetyl‐CoA deficits in thiamine deficiency‐evoked cholinergic encephalopathies. However, disturbances pathways metabolism leading to appearance remain unknown. Therefore, aim this work was investigate alterations concentration distribution acetylcholine brain nerve terminals, caused by deficits. They were induced pyrithiamine, a potent inhibitor...
Abstract Neopterin is one of biochemical markers immune activity, which seems to be useful in monitoring inflammatory diseases. Increased serum concentration neopterin observed conditions with cell-mediated response involvement. In clinical practice, evaluation applied medical such as: infectious diseases, oncology, rheumatology, transplantology, transfusiology, cardiology, neurology and many others. can detected serum, urine, cerebrospinal fluid, synovial saliva, ascitic bile, pancreatic...
Mechanisms of preferential loss cholinergic neurons in the course neurodegenerative diseases are unknown. Therefore, we investigated whether differentiation-evoked changes acetyl-CoA and acetylcholine metabolism contribute to susceptibility neuroblastoma cytotoxic effects Al. In SN56 cells differentiated with retinoic acid dibutyryl cAMP (DC), pyruvate utilization content were lower level higher than nondifferentiated (NC), respectively. DC Al Ca accumulations 50% 100%, respectively NC....
The rate of acetylcholine (ACh) synthesis was found to depend on the activity choline acetyltransferase (ChAT) and concentrations two substrates this enzyme, acetyl-CoA. In SN56 cells treated for 3 days with 1 mM dbcAMP activities ChAT acetylcholinesterase (AChE) were elevated. It accompanied by an increased ATP-citrate lyase (ACL)—an enzyme responsible provision part acetyl-CoA ACh in cholinergic neurons. contrast lactate dehydrogenase (LDH) pyruvate (PDH) reduced dbcAMP. Treatment 0.001...
Al complexes are known to accumulate in extra- and intracellular compartments of the brain course different encephalopathies. In this study possible effects accumulation cytoplasmic compartment on mitochondrial metabolism were investigated. Al, like Ca, inhibited pyruvate utilization as well citrate oxoglutarate by whole mitochondria. Potencies Ca2+(total) 10-20 times stronger than those Al. decreased acetyl-CoA content a concentration-dependent manner, along with an equivalent rise free CoA...
Excessive blood platelet activity contributes to vascular complications in diabetic persons. Increased acetyl-CoA platelets from persons has been suggested be a cause of this hyperactivity. We therefore investigated whether L-carnitine, which up-regulates metabolism muscles and brain, may affect function healthy individuals.We obtained measured concentrations, malonyl dialdehyde (MDA) synthesis, aggregation the absence presence L-carnitine. Activities selected enzymes involved glucose were...
The pyruvate-derived acetyl-CoA is a principal direct precursor substrate for bulk energy synthesis in the brain. Deficits of pyruvate dehydrogenase neocortex are common features Alzheimer's disease and other age-related encephalopathies humans. Therefore, amyloid-β overload brains diverse transgenic model animals was investigated as one neurotoxic compounds responsible inhibition yielding deficits cholinergic neurotransmission cognitive functions. Brains aged, 14–16-month-old Tg2576 mice...
The aim of this study was to evaluate the effects immunising farm animals with Histophilus somni recombinant heat shock protein 60kDa (H. rHsp60) in field conditions. Fifty piglets, 10 calves and 30 hens were immunised twice, same number each species used as control. humoral immune response evaluated using ELISA piglets (IgG, IgA IgM) (IgG1, IgG2 sera hen egg yolks (IgY). Cell-mediated responses skin test. Concentrations serum haptoglobin plasma fibrinogen calves, daily weight gain well...
Mild thiamine deficiency aggravates Zn accumulation in cholinergic neurons. It leads to the augmentation of toxicity by its interaction with enzymes energy metabolism. Within this study, we tested effect on microglial cells cultivated a thiamine-deficient medium, containing 0.003 mmol/L vs. 0.009 control medium. In such conditions, subtoxic 0.10 concentration caused non-significant alterations survival and metabolism N9 cells. Both activities tricarboxylic acid cycle acetyl-CoA level were...
Acetylcholine and acetyl-CoA metabolism in nerve terminals isolated from rat brain were found to be affected by several neurotoxic neuroprotective agents, such as aluminium, nitric oxide, β-hydroxybutyrate, verapamil thiamine deficiency. The changes evoked these factors Ca<sup>2+</sup>-dependent acetylcholine release highly significantly correlated (r = 0.98) with concentration of synaptoplasmic acetyl-CoA. On the other hand, same experimental conditions, no correlation was...
STREPROZOTOCIN diabetes and extracerebral insulin affect acetyl-CoA acetylcholine metabolism in the brain. In present study we have shown that pyruvate utilization, content ACh synthesis nerve terminals from diabetic rats were 45, 30 50%, respectively, higher than healthy animals. Treatment with normalized utilization but did not decrease level. 3-Hydroxybutyrate control rats. However, animals, 3-hydroxybutyrate significantly increased supply of for synthesis. These data provide evidence...