A5088970798- Alzheimer's disease research and treatments
- Amyotrophic Lateral Sclerosis Research
- SARS-CoV-2 and COVID-19 Research
- Parkinson's Disease Mechanisms and Treatments
- Dementia and Cognitive Impairment Research
- Prion Diseases and Protein Misfolding
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Tryptophan and brain disorders
- SARS-CoV-2 detection and testing
- Reproductive tract infections research
- Genetic Neurodegenerative Diseases
- Infection Control and Ventilation
- Malaria Research and Control
- Cerebrospinal fluid and hydrocephalus
- Mosquito-borne diseases and control
- Sex work and related issues
- Neurogenetic and Muscular Disorders Research
- Advanced Neuroimaging Techniques and Applications
- COVID-19 and Mental Health
- COVID-19 epidemiological studies
- Nuclear Receptors and Signaling
- CNS Lymphoma Diagnosis and Treatment
- Advanced Proteomics Techniques and Applications
- Cervical Cancer and HPV Research
KTH Royal Institute of Technology
2018-2025
Science for Life Laboratory
2018-2025
Abstract SARS-CoV-2 may pose an occupational health risk to healthcare workers. Here, we report the seroprevalence of antibodies, self-reported symptoms and exposure among workers at a large acute care hospital in Sweden. The IgG antibodies against was 19.1% 2149 recruited between April 14th May 8th 2020, which higher than reported regional during same time period. Symptoms associated with were anosmia (odds ratio (OR) 28.4, 95% CI 20.6–39.5) ageusia (OR 19.2, 14.3–26.1). Seroprevalence also...
Abstract Background Amyloid and tau aggregates are considered to cause neurodegeneration consequently cognitive decline in individuals with Alzheimer’s disease (AD). Here, we explore the potential of cerebrospinal fluid (CSF) proteins reflect AD pathology decline, aiming identify biomarkers for monitoring outcomes disease-modifying therapies targeting these aggregates. Method We used a multiplex antibody-based suspension bead array measure levels 49 CSF from Swedish GEDOC memory clinic...
Abstract Accurate diagnosis and monitoring of neurodegenerative diseases require reliable biomarkers. Cerebrospinal fluid (CSF) proteins are promising candidates for reflecting brain pathology; however, their diagnostic utility may be compromised by natural variability between individuals, weakening association with disease. Here, we measured the levels 69 pre-selected in cerebrospinal using antibody-based suspension bead array technology a multi-disease cohort 499 individuals disorders...
Abstract Background The clinical presentations of frontotemporal dementia (FTD) are diverse and overlap with other neurological disorders. There are, as today, no biomarkers in practice for diagnosing the Here, we aimed to find protein markers cerebrospinal fluid (CSF) from patients FTD, presymptomatic mutation carriers non-carriers. Methods Antibody suspension bead arrays were used analyse 328 proteins CSF behavioural variant FTD (bvFTD, n = 16) progressive primary aphasia (PPA, 13), well...
Abstract Objective Decreased amyloid beta (Aβ) 42 together with increased tau and phospho‐tau in cerebrospinal fluid (CSF) is indicative of Alzheimer’s disease (AD). However, the molecular pathophysiology underlying slowly progressive cognitive decline observed AD not fully understood it known what other CSF biomarkers may be altered early stages. Methods We utilized an antibody‐based suspension bead array to analyze levels 216 proteins from patients, patients mild impairment (MCI), controls...
The COVID-19 pandemic poses an immense need for accurate, sensitive and high-throughput clinical tests, serological assays are needed both overarching epidemiological studies evaluating vaccines. Here, we present the development validation of a multiplex bead-based assay.More than 100 representations SARS-CoV-2 proteins were included initial evaluation, including antigens produced in bacterial mammalian hosts as well synthetic peptides. five best-performing antigens, three representing spike...
Depot medroxyprogesterone acetate (DMPA) is an injectable hormonal contraceptive used by millions of women worldwide. However, experimental studies have associated DMPA use with genital epithelial barrier disruption and mucosal influx human immunodeficiency virus (HIV) target cells. We explored the underlying molecular mechanisms these findings. Ectocervical biopsies cervicovaginal lavage (CVL) specimens were collected from HIV-seronegative Kenyan sex workers using ( n = 32) or regularly...
Abstract Background SARS-CoV-2 may pose an occupational health risk to care workers, but the prevalence of infections in this population is unknown. We examined seroprevalence antibodies among workers at a large acute hospital Stockholm, Sweden. determined correlations between seroprevalence, self-reported symptoms and exposure SARS-CoV-2. Methods findings All employees Danderyd Hospital (n=4375) were invited participate cross-sectional study. 2149 from all departments enrolled study April...
A detailed understanding of the pathological processes involved in genetic frontotemporal dementia is critical order to provide patients with an optimal future treatment. Protein levels CSF have potential reflect different pathophysiological brain. We aimed identify and evaluate panels proteins separate symptomatic individuals from without clinical symptoms (unaffected), as well presymptomatic mutation non-carriers.A multiplexed antibody-based suspension bead array was used analyse 111...
Abstract Background Cognitive impairment is common in patients with PD. Core markers of Alzheimer's dementia have been related also to PD dementia, but no disease‐specific signature predict exists date. Objectives The aim this study was investigate CSF associated cognition early Methods A high‐throughput suspension bead array examined 216 proteins 74 the AETIONOMY project. function assessed Repeatable Battery for Assessment Neuropsychological Status, Montreal Assessment, and Mini‐Mental...
The majority of studies characterizing female genital tract microbiota have focused on luminal organisms, while the presence and impact tissue-adherent ectocervical remain incompletely understood. Studies tissue-associated bacteria in gastrointestinal suggest that these communities may distinct roles health disease. Here, we performed a multi-omics characterization paired tissue samples collected from cohort Kenyan sex workers.We identified bacterial microbiome, with higher alpha diversity...
The intimate interaction between the pathophysiology of human host and biology Plasmodium falciparum parasite results in a wide spectrum disease outcomes malaria. Development severe is associated with progressively augmented imbalance pro- anti-inflammatory responses to high loads sequestration parasitized erythrocytes. Although these phenomena collectively constitute common denominators for variety discrete malaria manifestations, mechanistic rationales behind discrepancies outcome are...
The clinical outcomes of primary immunodeficiencies (PIDs) are greatly improved by accurate diagnosis early in life. However, it is not common to consider PIDs before the manifestation severe symptoms. Including nation-wide newborn screening programs will potentially improve survival and provide better disease management preventive care PID patients. This calls for detection biomarkers blood use dried spot samples, which a part routine worldwide. Here, we developed method based on multiplex...
Increased knowledge of the evolution molecular changes in neurodegenerative disorders such as Alzheimer's disease (AD) is important for understanding pathophysiology and also crucial to be able identify validate biomarkers. While several biological that occur early development have already been recognized, need further characterization pathophysiological mechanisms behind AD still remains.In this study, we investigated cerebrospinal fluid (CSF) levels 104 proteins 307 asymptomatic...
Background The cervicovaginal epithelial barrier is crucial for defending the female reproductive tract against sexually transmitted infections. Hormones, specifically estradiol and progesterone, along with their respective receptor expressions, play an important role in modulating this barrier. However, influence of progesterone on gene protein expression ectocervical mucosa naturally cycling women not well understood. Methods Mucosal blood samples were collected from Kenyan sex workers at...
To evaluate the prognostic potential of neurofilament medium chain (NfM) in CSF from patients with ALS and explore its relationship extensively studied light (NfL) phosphorylated heavy (pNfH).
Abstract The extent that antibodies to SARS-CoV-2 may protect against future virus-associated disease is unknown. We invited all employees (n = 15,300) at work the Karolinska University Hospital, Stockholm, Sweden participate in a study examining SARS-Cov-2 relation registered sick leave. For consenting 12,928 healthy hospital could be determined and compared participant leave records. Subjects with viral serum were not excess risk for (adjusted odds ratio (OR) controlling age sex: 0.85 [95%...
Abstract Background Whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positivity among asymptomatic subjects reflects past or future disease may be difficult to ascertain. Methods We tested 9449 employees at Karolinska University Hospital, Stockholm, Sweden for SARS-CoV-2 RNA and antibodies, linked the results sick leave records, determined associations with using multinomial logistic regression. Results Subjects high amounts of virus, indicated by polymerase chain...
Abstract Background Plasma biomarkers reflecting the pathology of frontotemporal dementia would add significant value to clinical practice, design and implementation treatment trials as well our understanding disease mechanisms. The aim this study was explore levels multiple plasma proteins in individuals from families with genetic dementia. Methods Blood samples 693 participants GENetic Frontotemporal Dementia Initiative were analysed using a multiplexed antibody array targeting 158...
Immunological correlates of natural resistance to HIV have been identified in HIV-exposed seronegative (HESN) individuals and include a low-inflammatory genital mucosal status. The cervicovaginal epithelium has not studied for such despite constituting an important barrier against sexual transmission. To fill this gap knowledge, we collected samples blood, cervical mononuclear cells, lavage, ectocervical tissue from Kenyan HESN sex workers (n = 29) controls 33). were analyzed by flow...
Abstract Background Accurate diagnosis and monitoring of neurodegenerative diseases requires reliable biomarkers. Cerebrospinal fluid (CSF) proteins hold promise for reflecting brain pathology, yet their utility may be compromised by natural variability between individuals, weakening association with disease impacting diagnostic accuracy. Method We measured the levels 69 in CSF from 499 individuals using an antibody-based suspension bead array technology. In this multi-disease cohort...
ABSTRACT Importance Aquaporin-4 (AQP4) plays a critical role in the glymphatic system, responsible for clearing brain solutes like Aβ peptides. Exploring AQP4 as an Alzheimer’s disease (AD) biomarker might aid understanding of AD neuropathology and monitor effects novel drug candidates on system. Objective To determine potential CSF early stage using newly established immunoassay. Design A discovery cohort (n = 157) (2010-2022), composed by patients, other neurodegenerative conditions...