A5033967441- Cell Adhesion Molecules Research
- Cardiac Ischemia and Reperfusion
- Platelet Disorders and Treatments
- Helicobacter pylori-related gastroenterology studies
- Caveolin-1 and cellular processes
- Mesenchymal stem cell research
- Cancer Cells and Metastasis
- Gastric Cancer Management and Outcomes
- Organ Transplantation Techniques and Outcomes
- Gastroesophageal reflux and treatments
- Eosinophilic Esophagitis
- Cardiac and Coronary Surgery Techniques
- Hematopoietic Stem Cell Transplantation
- Galectins and Cancer Biology
- Acute Myocardial Infarction Research
- Atherosclerosis and Cardiovascular Diseases
- Proteoglycans and glycosaminoglycans research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Liver physiology and pathology
- Microfluidic and Bio-sensing Technologies
- Cerebrovascular and Carotid Artery Diseases
- Tissue Engineering and Regenerative Medicine
- Signaling Pathways in Disease
- Antiplatelet Therapy and Cardiovascular Diseases
- Nitric Oxide and Endothelin Effects
Indo Soviet Friendship College of Pharmacy
2025
University of Birmingham
2014-2024
University of Michigan
2024
University College Birmingham
2019
Myongji Hospital
2019
Hanyang University
2019
Azienda Ospedaliera Universitaria Senese
2019
NIHR Surgical Reconstruction and Microbiology Research Centre
2019
St James's University Hospital
2019
University of Nottingham
2018
The role of Rac family proteins in platelet spreading on matrix under static and flow conditions has been investigated by using Rac-deficient platelets. Murine platelets form filopodia undergo limited fibrinogen independent Rac1 Rac2. In the presence thrombin, marked lamellipodia formation is observed fibrinogen, which abrogated absence Rac1. However, not required for thrombin-induced aggregation or elevation F-actin levels. Formation collagen laminin also Rac1-dependent. Analysis adhesion...
IL-17 secreting CD4 (Th17) and CD8 (Tc17) T cells have been implicated in immune-mediated liver diseases, but the molecular basis for their recruitment positioning within is unknown.The phenotype migratory behaviour of human liver-derived Th17 Tc17 were investigated by flow cytometry chemotaxis flow-based adhesion assays. The murine to was studied vivo using intra-vital microscopy.IL-17(+) comprised 1-3% cell infiltrate inflammatory diseases included both cells. They expressed RORC IL-23...
Interactions between platelets, leukocytes and the vessel wall provide alternative pathological routes of thrombo-inflammatory leukocyte recruitment. We found that when platelets were activated by a range agonists in whole blood, they shed platelet-derived extracellular vesicles which rapidly preferentially bound to blood monocytes compared other leukocytes. Platelet-derived vesicle binding was initiated P-selectin-dependent adhesion stabilised phosphatidylserine. These interactions resulted...
Tetraspanin CD151 is associated with laminin-binding integrins and controls tumor cell migration invasion. By analyzing responses of breast cancer cells to various growth factors, we showed that depletion specifically attenuates transforming factor beta1 (TGFbeta1)-induced scattering proliferation in three-dimensional Matrigel. CD151-dependent requires its association either alpha3beta1 or alpha6 integrins, but it independent the recruitment tetraspanin-enriched microdomains. We also found...
Anti-myeloperoxidase (anti-MPO) antibodies have been implicated in the pathogenesis of small-vessel vasculitis, but molecular mechanisms by which these contribute to disease are unknown. For determination how anti-MPO affect inflammatory cell recruitment intravital microscopy was used monitor leukocyte behavior accessible cremasteric microvessels under various experimental conditions. After local pretreatment cremaster muscle with cytokines (TNF-alpha, IL-1beta, or keratinocyte-derived...
Human bone marrow mesenchymal stem cells (hMSCs) have shown benefit in clinical trials of patients with liver disease. Efficient delivery to target organs is critical improving their effectiveness. This requires an understanding the mechanisms governing cellular engraftment into liver. Binding hMSCs normal/injured tissue, purified extracellular matrices, and human hepatic sinusoidal endothelial (HSECs) were quantified static flow conditions. To define underpinning hMSC interactions,...
Background & AimsThere is growing interest in the use of bone marrow cells to treat liver fibrosis, however, little known about their antifibrotic efficacy or identity effector cell(s). Sphingosine-1-phosphate (S1P) mediates egress immune from lymphoid organs into lymphatic vessels; we investigated its role response hematopoietic stem (HSCs) fibrosis mice.MethodsPurified (c-kit+/sca1+/lin-) HSCs were infused repeatedly mice undergoing fibrotic injury. Chronic injury was induced BoyJ by...
Cells of the monocyte lineage are most abundant inflammatory cells found in atherosclerotic lesions. Dominance infiltrate by monocytes indicates that there is a disease-driven mechanism supporting their selective recruitment. Previous studies have demonstrated interactions between endothelial (ECs) and platelets may promote In this study, we sought to expand on knowledge using complex coculture model diseased vessel wall. Using primary human an vitro flow-based adhesion assay, secretory...
Following myocardial infarction (MI), elderly patients have a poorer prognosis than younger patients, which may be linked to increased coronary microvessel susceptibility injury. Interleukin-36 (IL-36), newly discovered proinflammatory member of the IL-1 superfamily, mediate this injury, but its role in injured heart is currently not known. We first demonstrated presence IL-36(α/β) and receptor (IL-36R) ischemia/reperfusion-injured (IR-injured) mouse hearts and, interestingly, noted that...
Mesenchymal stem cells (MSCs) have shown therapeutic promise in many experimental and clinical models of inflammation. However, a commonly reported feature MSC transplantation is poor homing to injured tissues. Previously, we that pretreatment with cytokines/chemical factors enhances hematopoietic SC adhesion within intestinal microvasculature following ischemia-reperfusion (IR) injury. Using intravital microscopy, the ability similar strategies enhance recruitment murine MSCs IR injury was...
Syndecans are heparan sulphate proteoglycans expressed by endothelial cells. Syndecan-3 is synovial cells of rheumatoid arthritis (RA) patients where it binds chemokines, suggesting a role in leukocyte trafficking. The objective the current study was to examine function syndecan-3 joint inflammation genetic deletion mice and compare with other tissues.Chemokine C-X-C ligand 1 (CXCL1) injected joints syndecan-3-/-and wild-type antigen-induced performed. For comparison chemokine administered...
Aspirin and platelet P2Y12 inhibitors, such as ticagrelor, suboptimally inhibit microvascular thrombosis during ST-elevation myocardial infarction. Glycoprotein (GP) IIb/IIIa inhibitors may further this but cause excessive bleeding.We investigated whether combination of glenzocimab, a GPVI inhibitor, with aspirin ticagrelor provides additional antithrombotic effects, has critical role in atherothrombosis minimal involvement hemostasis.We the effects glenzocimab (monoclonal antibody Fab...
Preliminary results of electrical impedance measurements in squamous and columnar epithelia rat human tissues are presented. The aim this work is to show the possibility differentiating these two types terms their characteristics. For measurements, we employed a 1.95-m-long, 3.2-mm-diameter, four-electrode probe designed be used transendoscopically diagnosis Barrett's esophagus (BE). BE condition which normal epithelium replaced by intestinal type. This metaplasia considered as premalignant...
ABSTRACT Objective : The role of collagen receptor complex GPVI‐FcR γ‐chain, PLCγ2 and LAT in laser‐induced thrombosis is unclear. Controversy surrounds whether exposed this model or dependent on thrombin. This study hypothesized that exposure plays a critical thrombus formation model, which was tested by investigating contributions FcR LAT, Methods Thrombi were monitored using intravital microscopy anesthetized wild‐type knockout mice. Hirudin (thrombin inhibitor) administered to γ‐chain...
Although the expression of PECAM-1 (CD31) on vascular and haematopoietic cells within bone marrow microenvironment has been recognized for some time, its physiological role this niche remains unexplored. In study we show that influences steady state hematopoietic stem cell (HSC) progenitor numbers in peripheral blood but not compartment. PECAM-1−/− mice have higher levels HSC progenitors compared to their littermate controls. We is required both order efficient transition between occur....
Evidence suggests haematopoietic stem cells (HSCs) can migrate to injured liver and influence tissue repair. However, mechanisms governing HSC recruitment hepatic microcirculation are poorly understood. These were investigated in vivo following ischaemia-reperfusion (IR) injury vitro using flow-based adhesion assays.Partial IR was induced anaesthetised WT or PECAM-1(-/-) mice for 90 min. Recruitment of systemically administered HSCs monitored effects function blocking antibodies against...
We investigated the adhesive behavior of mesenchymal stem cells (MSC) in blood, which might influence their fate when infused as therapy. Isolated human bone marrow MSC (BMMSC) or umbilical cord (UCMSC) adhered efficiently from flow to matrix proteins, collagen, fibronectin, but did not adhere endothelial selectins. However, suspended BMMSC no longer while UCMSC along with many aggregated platelets. Neither fibronectin flowing although surface become coated a platelet monolayer. induced...
Adequate microcirculatory perfusion, and not just opening of occluded arteries, is critical to salvage heart tissue following myocardial infarction. However, the degree microvascular perfusion taking place known, limited primarily by an inability directly image coronary microcirculation in a beating vivo. Haematopoietic stem/progenitor cells (HSPCs) offer potential therapy but little known about their homing dynamics at cellular level whether they protect microvessels. This study used...