MicroRNAs (miRNAs) are emerging as critical regulators in the pathological process of cerebral ischemia/reperfusion injury. miRNAs play an important role regulating neuronal survival. miR-135b-5p has been reported miRNA cell apoptosis. However, survival remains poorly understood. Here, we aimed to investigate ischemia/ reperfusion using vitro model oxygen-glucose deprivation and reoxygenation-(OGD/R) induced neuron injury.miRNA, mRNA protein expression was detected by real-time quantitative...

The aim of the research was to investigate anti-inflammatory mechanism Pulsatillae Decoction (PD), levels interleukin (IL)-6, IL-8, E-selectin, and thromboxane B2 (TXB2) secreted by cultured rat intestinal microvascular endothelial cells (RIMECs) were determined after treatment with its active ingredients, namely anemoside B4, anemonin, berberine, jatrorrhizine, palmatine, aesculin, esculetin. RIMECs challenged 1 μg/mL lipopolysaccharide (LPS) for 3 h, then treated each seven ingredients at...

Abstract To investigate the pharmacological mechanism of traditional Chinese medicine, Pulsatilla decoction (PD), levels nitric oxide (NO), endothelin‐1 (ET‐1), tumor necrosis factor‐ α (TNF‐ ), and interleukin‐1 (IL‐1 ) secreted by cultured rat intestinal microvascular endothelial cells (RIMECs) were determined after treatment with PD its seven active ingredients, namely anemoside B 4 , anemonin, berberine, jatrorrhizine, palmatine, aesculin, esculetin. RIMECs challenged lipopolysaccharide...

Mastitis gives rise to big financial burden farm industry (mainly dairy production) and public health. Its incidence is currently high therefore, highly effective treatments for therapy, especially with natural products are required. Taraxacum officinale has been reported use anti-inflammation. However, its effect on endothelium during mastitis not reported.We firstly established inflammation experimental model of rat mammary microvascular endothelial cells (RMMVECs). We evaluated the...

Our previous studies showed that the anti-inflammatory effects of Paeonia lactiflora roots extract may be mediated, at least in part, through its gallic acid content, and this effect regulated part by an inhibition on cAMP-phosphodiesterase (PDE). To explore mechanism, influence neutrophils PDE4 activity expression, TNF-α IL-6 content rat arthritis model were further studied. gene express calculated respectively substrate cAMP change examined with HPLC real-time RT-PCR. The concentration...

Enterotoxigenic Escherichia coli (ETEC)-induced diarrhea is a devastating disease and one of the third leading causes infectious disease-associated mortalities worldwide. Despite recent advances in identification association between gut microbiota diarrhea, lack understanding exists on status rats treated with ETEC. In present study, rat model (E.) O101-induced was established. The incidence index, as well histological changes, were assessed. addition, Illumina MiSeq sequencing V3-V4...

In the framework of thermo-electro-elasticity, present paper investigates singular behaviors interface corners, cracks, composite wedges and spaces for one-dimensional hexagonal quasicrystal. The stress function temperature variation can be described as exponential form with a view to heat flux singularities. Based on Stroh formalism, analytical expressions orders are easily established by simple multiplication crucial matrix. Numerical examples given some general cases including single,...

Quasicrystals have aroused great interest and argument among researchers due to their unique atomic configurations. In this paper, based on the Stroh formalism Barnett-Lothe matrices, we investigate problems of two-dimensional piezoelectric quasicrystal composite wedges spaces subjected different loadings, such as line force dislocation. The special cases semi-infinite spaces, infinite bi-material are also taken into consideration. We derive analytical expressions displacements stresses by...

The aim of this study was to investigate the traditional meridian theory using speckle laser blood flow scanning technology observe microcirculation Hegu acupoint area after acupuncture stimulation on distant points. An observational conducted microvascular perfusion (LI4) and control points acupuncturing Quchi (LI11). Thirty healthy volunteers (mean age<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...

IFIT1 (also known as ISG56) is a member of the interferon-inducible protein with tetratricopeptide repeats (IFITs) family. IFITs are strongly induced by type I interferon (IFN), double-stranded RNA and virus infection. Here, we investigated expression in human umbilical vein endothelial cells (HUVECs) bronchus epithelial (BEAS-2Bs) H9N2 inactivated viral particle at different time points. We also effect infection on IFN-α/β production, assessed whether hemagglutinin or neuraminidase...

Gut microbiota is critical as either the maker of risk or contributor therapy on bacterial infection. Both traditional Chinese medicine compound, Pulsatilla Decoction (PD) and antibiotic, Levofloxacin Hydrochloride (LVX) have antibacterial activity. However, how changes gut after PD LVX treatment hitherto not explicated. Herein, we aim to compare Escherichia coli induced Results showed that exhibited a valid therapeutic approach for E. infection, which also an intestinal protection had less...

Most studies on spinal cord neuronal injury have focused tissue histology and the expression of nerve cell damage repair-related genes. The importance microcirculation is often ignored in repair research. Therefore, this study, we established a rat model intervertebral disc extrusion by inserting silica gel pad into left ventral surface T13. Electroacupuncture was used to stimulate bilateral Zusanli point (ST36) Neiting (ST44) for 14 days. Compared with control animals, blood flow first...

To observe capillary blood flow at acupoints during acupuncture treatment of primary dysmenorrhea and gain new insights into its analgesic mechanism.Patients with were enrolled randomly assigned to a or control group. Subjects' symptoms differentiated various Traditional Chinese Medicine (TCM) syndromes treated for 10 sessions puncturing self-pressing right-hand Hegu (LI 4), adding other based on syndrome. Laser speckle was used compare the change in vasomotor amplitude perfusion capillaries...

Streptococcus pneumoniae is a clinically important pathogen responsible for significant morbidity and mortality worldwide. Disruption of the host gut microbiota by antibiotics reduces pulmonary resistance to S. pneumoniae. The aim our study was determine potential role TLR4 in reduced following disruption. Wild-type TLR4-deficient mice were given broad-spectrum 3 weeks oral gavage disrupt microbiota, subsequently inoculated intra-nasally with extent decline both animal groups evaluated terms...