A5027067638- COVID-19 Clinical Research Studies
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Chemokine receptors and signaling
- Cancer Cells and Metastasis
- Diabetes and associated disorders
- CAR-T cell therapy research
- Liver Disease Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Food Allergy and Anaphylaxis Research
- Monoclonal and Polyclonal Antibodies Research
- Chemical Reactions and Isotopes
- Bat Biology and Ecology Studies
- Berberine and alkaloids research
- Glycosylation and Glycoproteins Research
- Cancer, Lipids, and Metabolism
- Mast cells and histamine
- Systemic Lupus Erythematosus Research
- Cervical Cancer and HPV Research
- Animal testing and alternatives
- Endoplasmic Reticulum Stress and Disease
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
Agency for Science, Technology and Research
2017-2023
Institute of Molecular and Cell Biology
2017-2023
Peter Doherty Institute
2023
The University of Melbourne
2023
Duke-NUS Medical School
2023
Genome Institute of Singapore
2018-2021
Non-alcoholic fatty liver disease (NAFLD) has been on a global rise. While animal models have rendered valuable insights to the pathogenesis of NAFLD, discrepancy with patient data still exists. Since non-alcoholic steatohepatitis (NASH) involves chronic inflammation, and CD4+ T cell infiltration is characteristic NASH patients, we established characterized humanized mouse model identify human-specific immune response(s) associated NAFLD progression. Immunodeficient mice engrafted human...
Cell state transitions control the functional behavior of cancer cells. Epithelial-to-mesenchymal transition (EMT) confers stem cell-like properties, enhanced tumorigenicity and drug resistance to tumor cells, while mesenchymal-epithelial (MET) reverses these phenotypes. Using high-throughput chemical library screens, retinoids are found be potent promoters MET that inhibit in basal-like breast cancer. defined by reprogramming lipid metabolism. Retinoids bind cognate nuclear receptors, which...
Recent development of multiple treatments for human hepatocellular carcinoma (HCC) has allowed the selection combination therapy to enhance effectiveness monotherapy. Optimal therapies is based on both HCC and its microenvironment. Therefore, it critical develop validate preclinical animal models testing clinical therapeutic solutions.We established cell line-based or patient-derived xenograft-based humanized-immune-system mouse with subcutaneous orthotopic HCC. Mice were injected...
In recent decades, chimeric antigen receptor (CAR)-engineered immune effector cells have demonstrated promising antileukemic activity. Nevertheless, their efficacy remains unsatisfactory on solid cancers, plausibly due to the influence of tumor microenvironments (TME). a novel mouse cancer model with humanized system, tumor-infiltrating immunosuppressive leukocytes and exhausted programmed death protein-1 (PD-1)high T were found, which better mimic patient TME, allowing screening assessment...
Mouse models have contributed to the bulk of knowledge on Systemic Lupus Erythematosus (SLE). Nevertheless, substantial differences exist between human and mouse immune system. We aimed establish characterise a SLE model mediated by Injection pristane into immunodeficient mice reconstituted with system (humanised mice) recapitulated key features, including: production anti-nuclear autoantibodies, lupus nephritis, pulmonary serositis. There was reduction in number lymphocytes peripheral...
Immune checkpoint blockade (ICB) monotherapy shows early promise for the treatment of nasopharyngeal carcinoma (NPC) in patients. Nevertheless, limited representative NPC models hamper preclinical studies to evaluate efficacy novel ICB and combination regimens. In present study, we engrafted biopsies non-obese diabetic-severe combined immunodeficiency interleukin-2 receptor gamma chain-null (NSG) mice established humanized mouse NPC-patient-derived xenograft (NPC-PDX) model successfully....
Recently a G-protein-coupled receptor, MAS Related GPR Family Member X2 (MRGPRX2), was identified as specific receptor on human mast cells responsible for IgE independent adverse drug reactions (ADR). Although murine homologue, Mrgprb2, has been this its affinity many ADR-causing drugs is poor making it difficult to undertake in vivo studies examine mechanisms of ADR and develop therapeutic strategies. Here, we have created humanized mice capable generating MRGPRX2-expressing MCs allowing...
Xenotransplantation of patient-derived AML (acute myeloid leukemia) cells in NOD-scid Il2rγ null (NSG) mice is the method choice for evaluating this human hematologic malignancy. However, existing models constructed using intravenous injection adult or newborn NSG have inferior engraftment efficiency, poor peripheral blood engraftment, are difficult to construct.Here, we describe an improved xenograft model where primary were injected into pups intrahepatically.Introduction from patients...
Abstract Bats are an important animal model with long lifespans, low incidences of tumorigenesis and ability to asymptomatically harbour pathogens. Currently, in vivo studies bats hampered due their reproduction rates. To overcome this, we transplanted bat cells from bone marrow (BM) spleen into immunodeficient mouse strain NOD-scid IL-2R −/− (NSG), have successfully established stable, long-term reconstitution immune mice (bat-mice). Immune functionality our bat-mouse was demonstrated...
Advancements in science enable researchers to constantly innovate and create novel biologics. However, the use of non-human animal models during development biologics impedes identification precise vivo interactions between human immune system treatments. Due lack this understanding, adverse effects are frequently observed healthy volunteers patients exposed potential clinical trials. In study, we evaluated compared known immunotoxic biologics, Proleukin®/IL-2 OKT3 humanized mice...
The COVID-19 pandemic has sickened millions, cost lives and devastated the global economy. Various animal models for experimental infection with SARS-CoV-2 have played a key role in many aspects of research. Here, we describe humanized hACE2 (adenovirus expressing hACE2) NOD-SCID IL2Rγ
Abstract The COVID-19 pandemic has sickened millions, cost lives and devastated the global economy. Various animal models for experimental infection with SARS-CoV-2 have played a key role in many aspects of research. Here, we describe humanized AdV-hACE2 NOD-SCID IL2Rγ-/- (NIKO) mouse model compared ancestral mutant (SARS-CoV-2-∆382) strains SARS-CoV-2. Immune cell infiltration, inflammation, lung damage pro-inflammatory cytokines chemokines was observed NIKO mice. Humanized mice infected WT...
Abstract Triple-negative breast cancer (TNBC), as immunohistochemically defined by its estrogen receptor (ER)-negative, progesterone (PR)-negative and human epidermal growth factor receptor-2 (HER2)-negative status, is an important subtype due to biologically aggressive behavior limited treatment options available. TNBC associated with overall poorer prognosis, higher risk of disease recurrence/progression shorter duration response, i.e., resistance. Treatment resistance may be largely...
Abstract Epstein-Barr virus (EBV)-associated nasopharyngeal carcinoma (NPC) is common in Southern China and South-East Asia. Immune checkpoint blockade (ICB) being studied NPC initial monotherapy ICB studies show early promise. Currently, lack of representative models limit the preclinical to evaluate efficacy safety novel combination regimens. Hence, there a need develop characterize mouse model with human immune system for testing immunotherapy. Here, we engrafted NPC-PDX immunodeficient...