Background Lung cancer is the deadliest form of malignancy and most common subtype non-small cell lung (NSCLC). Hypoxia a typical feature solid tumor microenvironment. In current study, we clarified effects hypoxia on stemness metastasis molecular mechanism.

Concerns were increasingly raised that several types of cancers overexpressed the nuclear factor erythroid 2-related 2 (Nrf2), which contributed strikingly to cancer biological capabilities and chemoresistance. However, role Nrf2 in tumor vascular biology had yet be mechanistically determined. Here, we investigated involvement glioblastoma (GB) angiogenesis hypoxia. First, detected overexpression correlated its protein level with microvessel density (MVD) human GB tissues. Then, established...

Transfer RNA-derived RNA fragments (tRFs) belong to non-coding RNAs (ncRNAs) discovered in most carcinomas. Although some articles have demonstrated the characteristics of tRFs gastric carcinoma (GC), underlying mechanisms still need be elucidated. Meanwhile, it was reported that MAPK pathway momentous GC progression. Thus we focused on investigating whether tRF-Glu-TTC-027 could act as a key role progression with regulation pathway. We collected data tRNA-derived expression profile from six...

tRNA derivatives have been identified as a new kind of potential biomarker for cancer. Previous studies that there were 30 differentially expressed tRNAs in breast cancer tissue with the high-throughput sequencing technique. This study aimed to investigate possible biological function and mechanism cells. One such tRF, 5’-tRF fragment tRF-17-79MP9PP (tRF-17) was screened this study, which is processed from mature tRNA-Val-AAC tRNA-Val-CAC. tRF-17 significantly low expression tissues serum....

Abstract Background As a new kind of non-coding RNAs (ncRNAs), tRNA derivatives play an important role in gastric carcinoma (GC). Nevertheless, the underlying mechanism were involved was rarely illustrated. Methods We screened out derivative, tRF-Val-CAC-016, based on tsRNA sequencing and demonstrated effect tRF-Val-CAC-016 exerted GC proliferation vitro vivo. applied Dual-luciferase reporter assay, RIP bioinformatic analysis to discover downstream target tRF-Val-CAC-016. Then CACNA1d...

The UHRF 1 protein is pivotal for DNA methylation and heterochromatin formation, leading to decreased expressions of tumor suppressor genes contributing tumorigenesis. However, the factors that modulate expression in colorectal cancer ( CRC ) remain unclear. Here we showed that, compared with corresponding normal tissues, was upregulated micro RNA ‐9 (miR‐9) downregulated tissues. inversely correlated overall survival rates patients . Overexpression miR‐9 cell lines significantly attenuated...

Background: DC-STAMP domain containing 1-antisense 1 (DCST1-AS1) is a long noncoding RNA (lncRNA) that up-regulated in triple-negative breast cancer (TNBC) tissues. Here, we attempt to investigate the oncogenic property of DCST1-AS1. Methods: LncRNA microarrays were used detect differentially expressed lncRNA cancerous Fluorescence situ hybridization assay was distribution DCST1-AS1 BT-549 and MDA-MB-231 cells. Lentiviral systems, inhibitors, siRNA overexpression plasmids for gain-...

Triple-negative breast cancer (TNBC) is a highly metastatic subtype, and the primary systemic treatment strategy involves conventional chemotherapy. DC-STAMP domain containing 1-antisense 1 (DCST1-AS1) long non-coding RNA that promotes TNBC migration invasion. Studying role of DCST1-AS1 in promoting epithelial-mesenchymal transition (EMT) chemoresistance will provide new for therapy. In present study, we found regulates expression or secretion EMT-related proteins E-cadherin, snail family...

BackgroundChemoresistance is a major obstacle for the effective treatment of lung adenocarcinoma (LAD). Forkhead box (FOX) proteins have been demonstrated to play critical roles in promoting epithelial-mesenchymal transition (EMT) and chemoresistance. However, whether FOX contribute acquisition EMT chemoresistance LAD remains largely unknown.MethodsFOX-A1 expression was measured cells tissues by qRT-PCR. The levels markers were detected western blotting immunofluorescence assay. interaction...

Metabolic reprogramming is an emerging hallmark and promising therapeutic target in cancer, fueling malignant cells regulating the tumor microenvironment as a metabolic checkpoint. Triple-negative breast cancer (TNBC), aggressive subtype with poor prognosis, marked by high recurrence rates chemotherapy resistance. However, heterogeneity cell-specific profiles TNBC remain underexplored. In this study, we analyzed expression prognostic impact of 87 pathways involving 1,668 genes, identifying...

Abstract Synthetic lethality (SL) has emerged as a highly promising therapeutic strategy, gaining significant attention in the field of cancer treatment recent years. The principle behind SL is that simultaneous loss or inhibition two genes results cell death, while only one gene does not. drugs exploit this mechanism by targeting partner an already inactivated mutated cells, inducing their death. This approach allows for precise genetic vulnerabilities specific to sparing normal tissues...

The aberrant expression of long noncoding RNAs (lncRNAs) has great impacts on cancer origination and progression.In the current study, a newly found lncRNA Z38, which was identified through combining experiments suppression subtractive hybridization (SSH) reverse dot-blotting, to have high in breast cancer.More importantly, inhibiting Z38 by gene silencing greatly suppressed cell proliferation tumorigenesis, treatment with siRNAs significantly induced apoptosis inhibited tumor growth.In...

Abstract Long non-coding RNAs (lncRNA) have been identified as key regulators of tumorigenesis and development. We aim to explore the biological functions molecular mechanisms lncRNA MIR200CHG in breast cancer. found that is highly expressed cancer tissues related tumor size histopathological grade. In vitro vivo experiments confirmed can promote proliferation, invasion, drug resistance. directly binds transcription factor Y-box binding protein-1 (YB-1), inhibits its ubiquitination...

Immune checkpoint inhibitors (ICIs) for non-small-cell lung cancer (NSCLC) are on the rise, but unfortunately, only a small percentage of patients benefit from them in long term. Thus, it is crucial to identify biomarkers that can forecast efficacy immunotherapy. We retrospectively studied 224 with NSCLC who underwent anti-PD-1 therapy. The role and clinical characteristics were assessed prognostic model. Only 14.3% had both programmed death ligand 1 (PD-L1) tumor mutational burden (TMB)...

Ubiquitin-like, containing PHD and RING finger domains 1 (UHRF1) has been reported to play an important role in breast carcinogenesis. This work investigated the correlation of UHRF1 DNA level plasma with clinical characteristics cancer its significance diagnosis. The expression primary tissue was examined by Western blot. levels mRNA tissues were determined accurate real-time quantitative PCR. associations variables evaluated using standard statistical methods. 229 patients showed higher...

Radiotherapy is the mainstay in treatment of prostate cancer. However, significant radioresistance castration-resistant cancer (CRPC) cells constitutes a main obstacle this disease. By using bioinformatic data mining methods, LOXL2 was found to be upregulated both androgen-independent cell lines and radioresistant tumor samples collected from patients with We speculate that may play an important role CRPC cells.The effect knockdown on radiosensitivity measured by clonogenic assay xenograft...

Obesity is known to affect cell-mediated immune responses. Recent studies have revealed that genetic polymorphisms in the fat mass and obesity associated (FTO) gene are related human obesity. We hypothesize this may also play a role risk of immune-related infectious diseases such as tuberculosis.This case-control study included 1625 pulmonary tuberculosis cases 1570 unaffected controls recruited from Jiangsu province China. Single nucleotide (SNPs), rs9939609 rs8050136, FTO were genotyped...