A5009656341- Bacillus and Francisella bacterial research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
- Bacterial Infections and Vaccines
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- COVID-19 epidemiological studies
- Pneumonia and Respiratory Infections
- Viral Infections and Vectors
- Hepatitis B Virus Studies
- Respiratory viral infections research
- Mosquito-borne diseases and control
- Monoclonal and Polyclonal Antibodies Research
- Biosensors and Analytical Detection
- Kawasaki Disease and Coronary Complications
- Microbial infections and disease research
- Molecular Biology Techniques and Applications
- Infective Endocarditis Diagnosis and Management
- Animal Disease Management and Epidemiology
- Microbial Inactivation Methods
- Virology and Viral Diseases
- Bacterial Genetics and Biotechnology
- Poxvirus research and outbreaks
- Congenital Ear and Nasal Anomalies
Centers for Disease Control and Prevention
2015-2024
Office of Readiness and Response
2024
The Centers
2024
National Center for Immunization and Respiratory Diseases
2010-2023
Response Biomedical (Canada)
2020
National Center for Environmental Health
2009
Johns Hopkins Medicine
2006
Johns Hopkins University
2006
University of Bern
1999
University of California, San Diego
1994
A general strategy is described for the de novo design of proteins. In this sequence locations hydrophobic and hydrophilic residues were specified explicitly, but precise identities side chains not constrained varied extensively. This was tested by constructing a large collection synthetic genes whose protein products designed to fold into four-helix bundle Each gene encoded different amino acid sequence, all sequences shared same pattern polar nonpolar residues. Characterization expressed...
Reported cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection likely underestimate the prevalence in affected communities. Large-scale seroprevalence studies provide better estimates proportion population previously infected.
Health care personnel (HCP) caring for patients with coronavirus disease 2019 (COVID-19) might be at high risk contracting SARS-CoV-2, the virus that causes COVID-19. Understanding prevalence of and factors associated SARS-CoV-2 infection among frontline HCP who COVID-19 are important protecting both their patients. During April 3-June 19, 2020, serum specimens were collected from a convenience sample worked 13 geographically diverse academic medical centers in United States, tested...
Compared with the volume of data on coronavirus disease 2019 (COVID-19) outbreaks among older adults, relatively few are available concerning COVID-19 in younger, healthy persons United States (1,2). In late March 2020, aircraft carrier USS Theodore Roosevelt arrived at port Guam after numerous U.S. service members onboard developed COVID-19. April, Navy and CDC investigated this outbreak, demographic, epidemiologic, laboratory findings a convenience sample 382 serving aboard reported study....
Most persons infected with SARS-CoV-2, the virus that causes coronavirus disease 2019 (COVID-19), develop virus-specific antibodies within several weeks, but antibody titers might decline over time. Understanding timeline of is important for interpreting SARS-CoV-2 serology results. Serum specimens were collected from a convenience sample frontline health care personnel at 13 hospitals and tested to during April 3-June 19, 2020, again approximately 60 days later assess this timeline. The...
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes disease 2019 (COVID-19), was first identified in Wuhan, China, December 2019, with subsequent worldwide spread. The US cases were January 2020. Methods To determine if SARS-CoV-2–reactive antibodies present sera prior to case United States on 19 2020, residual archived samples from 7389 routine blood donations collected by American Red Cross 13 17 2020 donors resident 9 states (California,...
These recommendations from the Advisory Committee on Immunization Practices (ACIP) update previous for anthrax vaccine adsorbed (AVA) (CDC. Use of in United States: Recommendations [ACIP]. MMWR 2000;49:1-20; CDC. response to terrorism: supplemental 2002;51:1024-6) and reflect status supplies States. This statement 1) provides updated information epidemiology; 2) summarizes evidence regarding effectiveness efficacy, immunogenicity, safety AVA; 3) pre-event preexposure use 4) postexposure AVA....
Abstract Importance Reported cases of SARS-CoV-2 infection likely underestimate the prevalence in affected communities. Large-scale seroprevalence studies provide better estimates proportion population previously infected. Objective To estimate antibodies convenience samples from several geographic sites United States. Design Serologic testing using residual sera obtained for routine clinical by two commercial laboratory companies. Setting Connecticut (CT), south Florida (FL), Missouri (MO),...
ABSTRACT Swine origin 2009 H1N1 influenza virus has spread globally to cause the first pandemic of 21st century. Serological studies can improve our understanding extent human infection and risk factors associated with transmission this virus. The “gold standard” for serodiagnosis is detection seroconversion between acute- convalescent-stage samples. However, timing seroepidemiological investigations often precludes collection truly acute-phase sera, requiring development serological...
.Streptococcus pneumoniae is a highly impactful bacterial pathogen on global scale. The principal pneumococcal virulence factor and target of effective vaccines its polysaccharide capsule, which there are many structurally distinct forms. Here we describe four strains three Mitis group commensal species (Streptococcus infantis, Streptococcus mitis, oralis) recovered from upper respiratory tract specimens adults in Kenya the United States that were PCR-positive for serotype 5 specific gene,...
Lethal toxin–neutralizing activity in serum after vaccination can provide cross-species predictions of survival against inhalation anthrax.
ABSTRACT A 3-dose (0, 1, and 6 months) intramuscular (3-IM) priming series of a human dose (HuAVA) dilutions up to 1:10 anthrax vaccine adsorbed (AVA) provided statistically significant levels protection (60 100%) against inhalation for 4 years in rhesus macaques. Serum anti-protective antigen (anti-PA) IgG lethal toxin neutralization activity (TNA) were detectable following single injection HuAVA or 1:5 AVA two injections diluted (1:10, 1:20, 1:40 AVA). Anti-PA TNA highly correlated...
Because of health concerns surrounding in utero and neonatal exposure to perchlorate, we developed a method for analyzing perchlorate the dried blood spots (DBS) newborns. Ion chromatography was interfaced with electrospray ionization tandem mass spectrometry measure levels DBS samples as low 0.10 ng/mL. Measurement indicated good precision (relative standard deviations ranging from 5.8% 16.2%) accuracy (% difference values -11.3% -12.1%). Perchlorate detectable 100% collected 100 These had...
Natural infection-induced humoral immunity to matrix protein 2 (M2) of influenza A viruses in humans is not fully understood. Evidence suggests that anti-M2 antibody responses following virus infection are weak and/or transient. We show the seroprevalence antibodies increased with age 317 serum samples from healthy individuals United States 2007-2008. Infection 2009 pandemic H1N1 (A[H1N1]pdm09) elicited a recall response M2 A(H1N1)pdm09 47% affected 118 tested. Anti-M2 were more robust among...
Cutaneous anthrax outbreaks occurred in Bangladesh from August to October 2009. As part of the epidemiological response and confirm diagnoses, serum samples were collected suspected case patients with observed cutaneous lesions. Anthrax lethal factor (LF), anti-protective antigen (anti-PA) immunoglobulin G (IgG), toxin neutralization activity (TNA) levels determined acute convalescent 26 first largest these outbreaks. LF (0.005–1.264 ng/mL) was detected 18 individuals. Anti-PA IgG TNA sera...
ABSTRACT Humoral and cell-mediated immune correlates of protection (COP) for inhalation anthrax in a rhesus macaque ( Macaca mulatta ) model were determined. The immunological survival data from 114 vaccinated 23 control animals exposed to Bacillus anthracis spores at 12, 30, or 52 months after the first vaccination. received 3-dose intramuscular priming series (3-i.m.) vaccine adsorbed (AVA) (BioThrax) 0, 1, 6 months. responses modulated by administering range dilutions. Together with...
• ZIKV IgM kits from InBios, NovaTec and Euroimmun were compared in 3 separate labs. InBios kit was most sensitive; Novatec specific. Concordance across the labs high for both + IgG kits. The NS1-based may be useful confirmation of infection a portion patients to reduce number PRNT's required.
The diagnosis of dengue disease, caused by the virus (DENV) (a flavivirus), often requires serologic testing during acute and early convalescent phases disease. Some symptoms DENV infection, such as nonspecific fever, are similar to those infection with SARS-CoV-2, that causes COVID-19. In studies few COVID-19 cases, positive immunoglobulin M (IgM) results were reported various tests, indicating possible cross-reactivity in these tests for SARS-CoV-2 infections (1,2). antibodies can...
Although COVID-19 vaccine efficacy (VE) studies have estimated antibody concentrations that correlate with protection from COVID-19, how these estimates compare to those generated in response SARS-CoV-2 infection is unclear. We assessed quantitative neutralizing and binding using standardized assays on serum specimens collected COVID-19-unvaccinated persons detectable antibodies.
Severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) is a novel human that was identified in 2019. SARS-CoV-2 infection results an acute, severe disease called 2019 (COVID-19). The emergence and rapid spread of has led to global public health crisis, which continues affect populations across the globe. Real time reverse transcription polymerase chain reaction (rRT-PCR) reference standard test for COVID-19 diagnosis. Serological tests are valuable tools serosurveillance...