A5050051435- Genomics, phytochemicals, and oxidative stress
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Glutathione Transferases and Polymorphisms
- Renal and related cancers
- Cytokine Signaling Pathways and Interactions
- Fibroblast Growth Factor Research
- Dermatologic Treatments and Research
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Multiple Sclerosis Research Studies
- Skin Protection and Aging
- Liver physiology and pathology
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Chromatin Dynamics
- Polyomavirus and related diseases
- Immune cells in cancer
- Tryptophan and brain disorders
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
- TGF-β signaling in diseases
- Hippo pathway signaling and YAP/TAZ
- Liver Disease Diagnosis and Treatment
- Macrophage Migration Inhibitory Factor
Klinikum rechts der Isar
2020-2024
Technical University of Munich
2021-2024
Broad Institute
2020-2021
Munich Cluster for Systems Neurology
2020-2021
Universitätsklinikum Erlangen
2020-2021
Brigham and Women's Hospital
2020-2021
Harvard University
2020-2021
Massachusetts General Hospital
2021
École Polytechnique Fédérale de Lausanne
2020
Friedrich-Alexander-Universität Erlangen-Nürnberg
2020
The liver is frequently exposed to insults, including toxic chemicals and alcohol, viral infection or metabolic overload. Although it can fully regenerate after acute injury, chronic damage causes fibrosis cirrhosis, which result in complete failure. In this study, we demonstrate that the NF-E2-related factor 2 (Nrf2) transcription protects from toxin-mediated damage. Repair of injury occurs a single treatment with hepatotoxin carbon tetrachloride (CCl(4)) was severely delayed Nrf2-deficient...
RNA sequencing (RNA-seq) has become a standard procedure to investigate transcriptional changes between conditions and is routinely used in research clinics. While differential expression (DE) analysis two been extensively studied, improved over the past decades, RNA-seq time course (TC) DE algorithms are still their early stages. In this study, we compare, for first time, existing TC tools on an extensive simulation data set validated best performing published data. Surprisingly, were...
Central nervous system (CNS)-resident cells such as microglia, oligodendrocytes and astrocytes are gaining increasing attention in respect to their contribution CNS pathologies including multiple sclerosis (MS). Several studies have demonstrated the involvement of pro-inflammatory glial subsets pathogenesis propagation inflammatory events MS its animal models. However, it has only recently become clear that underlying heterogeneity microglia can not drive inflammation, but also lead...
The Nrf2 transcription factor is a key player in the cellular stress response through its regulation of cytoprotective genes. In this study we determined role Nrf2-mediated gene expression keratinocytes for skin development, wound repair, and carcinogenesis. To overcome compensation by related Nrf1 Nrf3 proteins, expressed dominant-negative mutant (dnNrf2) epidermis transgenic mice. functionality transgene product was verified vivo using mice doubly dnNrf2 an Nrf2-responsive reporter gene....
Abstract Multiple Sclerosis (MS) is a chronic autoimmune inflammatory disorder of the central nervous system (CNS). Current therapies mainly target processes during acute stages, but effective treatments for progressive MS are limited. In this context, astrocytes have gained increasing attention as they capacity to drive, also suppress tissue-degeneration. Here we show that upregulate immunomodulatory checkpoint molecule PD-L1 CNS inflammation in response aryl hydrocarbon receptor and...
Addiction to UV light and opioids is increased by VitD deficiency, suggesting that a feedback loop promotes its synthesis.
Summary Loss of keratinocyte differentiation is a leading cause in several skin diseases and needs to be controlled adult homeostasis by for instance growth factors proteases. Among them, we studied the role isoform-rich dermokine – wound- tumour-related matrix metalloproteinase 10 substrate via functional multi-omics. We generated isoform-dependent knockouts three dimensional (3D) organotypic cultures analyzed changes their proteome phosphoproteome quantitative mass spectrometry. Through...
Chinese hamster ovary (CHO) cells are the commonly used mammalian host system to manufacture recombinant proteins including monoclonal antibodies. However unfavorable non-human glycoprofile displayed on CHO-produced antibodies have negative impacts product quality, pharmacokinetics, and therapeutic efficiency. Glycoengineering such as genetic elimination of genes involved in glycosylation pathway CHO is a viable solution but constrained due longer timeline laborious workflow. Here, this...
<h3>Objective</h3> The relationship between serum aryl hydrocarbon receptor (AHR) agonistic activity levels with disease severity, its modulation over the course of relapsing-remitting MS (RRMS), and regulation in progressive (PMS) are unknown. Here, we report analysis AHR cross-sectional longitudinal samples patients RRMS PMS. <h3>Methods</h3> In a investigation, total 36 control diagnosed noninflammatory diseases, 84 RRMS, 35 secondary (SPMS), 41 primary (PPMS) were included this study....
Multiple sclerosis (MS) is an autoimmune inflammatory disease of the CNS that characterized by demyelination and axonal degeneration. Although several established treatments reduce relapse burden, effective to halt chronic progression are scarce. Single-cell transcriptomic studies in MS its animal models have described astrocytes their spatial functional heterogeneity as important cellular determinants disease. We combined single-cell transcriptome data small-molecule screens primary mouse...
To evaluate the aryl hydrocarbon receptor (AHR)-dependent transforming growth factor alpha (TGF-α)/vascular endothelial B (VEGF-B) ratio, which regulates effects of metabolic, dietary, and microbial factors on acute chronic CNS inflammation, as a potential marker in multiple sclerosis (MS).TGF-α, VEGF-B, AHR agonistic activity were determined serum 252 patients with relapsing-remitting (RR) MS, primary secondary progressive well during active disease (clinically isolated syndrome [CIS] RRMS...
The fibroblast growth factor (FGF) and the transforming factor-β (TGF-β) pathways are both involved in maintenance of human embryonic stem cells (hESCs) regulate onset their differentiation. Their converging functions have suggested that these might share a wide range overlapping targets. Published studies focused on long-term effects (24–48 h) FGF TGF-β inhibition hESCs, identifying direct indirect target genes. In this study, we earliest transcriptome changes occurring between 3 9 h after...
The programmed cell death protein 1/programmed ligand 1 axis plays an important role in the adaptive immune system and has influence on neoplastic inflammatory diseases, while its multiple sclerosis is unclear. Here, we aimed to analyse expression patterns of peripheral blood mononuclear cells their soluble variants patients controls, determine correlation with clinical disability disease activity. In a cross-sectional study, performed in-depth flow cytometric immunophenotyping analysed...